Kinetic characterisation of recombinant Corynebacterium glutamicum NAD +-dependent LDH over-expressed in E. coli and its rescue of an lldD - phenotype in C. glutamicum: The issue of reversibility re-examined

Archives of Microbiology

Volumn 193, Issue 10, 2011, Pages 731-740

  Sharkey, Michael A ^a   Maher, Marcus A ^a   Guyonvarch, Armel ^b,c   Engel, Paul C ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b UNIVERSITÉ PARIS SACLAY   (France)

^c CNRS   (France)

Author keywords

Corynebacterium glutamicum; Enzyme kinetics; Genetic complementation; Lactate dehydrogenase; Unidirectional reaction

Indexed keywords

CGLDH PROTEIN; FRUCTOSE 1,6 BISPHOSPHATE; ISOPROPYL THIOGALACTOSIDE; LACTATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL KINETICS; BACTERIAL STRAIN; CARBON SOURCE; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; ESCHERICHIA COLI; GENE EXPRESSION; ION EXCHANGE CHROMATOGRAPHY; LDH GENE; MICHAELIS MENTEN KINETICS; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PRIORITY JOURNAL;

BACTERIAL PROTEINS; CLONING, MOLECULAR; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVATION; ESCHERICHIA COLI; FRUCTOSEDIPHOSPHATES; GENE EXPRESSION REGULATION, BACTERIAL; HYDROGEN-ION CONCENTRATION; KINETICS; L-LACTATE DEHYDROGENASE; LACTIC ACID; MUTATION; NAD; OPEN READING FRAMES; OXIDATION-REDUCTION; PYRUVIC ACID; RECOMBINANT PROTEINS;

CORYNEBACTERIUM GLUTAMICUM; CORYNEBACTERIUM GLUTAMICUM ATCC 13032;

EID: 82455168005     PISSN: 03028933     EISSN: 1432072X     Source Type: Journal    
DOI: 10.1007/s00203-011-0711-z     Document Type: Article

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