New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR

Biological Procedures Online

Volumn 7, Issue 1, 2005, Pages 70-92

  Gallup, Jack M ^a   Kawashima, Kenji ^b   Lucero, Ginger ^c   Ackermann, Mark R ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^c INVITROGEN CORPORATION   (United States)

Author keywords

Gene expression; Immunohistochemistry; Polymerase chain reaction; Sheep

Indexed keywords

RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CALIBRATION; DNA MICROARRAY; FROZEN SECTION; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; LASER CAPTURE MICRODISSECTION; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; VALIDATION PROCESS;

ARCTURUS; OVIS ARIES;

EID: 25444476683     PISSN: 14809222     EISSN: None     Source Type: Journal    
DOI: 10.1251/bpo107     Document Type: Article

References (24)

1. Webb T. Laser Capture Microdissection Comes Into Mainstream Use. J Natl Cancer Inst 2000; 92(21):1710-1711.
2. Invitrogen Life Technologies Instruction Manual. SuperScript III CellsDirect cDNA Synthesis System (for Catalog Nos. 18080-200 and 18080-300) Version A, 25-0731 14 May 2004; Version A; 1-5.
3. Use of glycogen or tRNA for reducing non-specific adherence of templates to Plastic. Applied Biosystems Incorporated 5700 User Manual 2000. Section 5, p.14.
4. Lee J, Duncan A, Warrick S. Techniques for Optimizing RT-PCR Reactions. PharmaGenomics 2003: pp. 54-55.
5. Dunphy J, Horvath A, Barcham G, Balic A, Bischof R, Meeusen E. Isolation, characterisation and expression of mRNAs encoding the ovine CC chemokines, monocyte chemoattractant protein (MCP)-1α and -2. Vet Immunol Immunopathol 2001; 82(3-4):153-164.
6. Grubor B, Gallup JM, Meyerholz DK, Crouch EC, Evans RB, Brogden KA, Lehmkuhl HD, Ackermann MR. Enhanced surfactant protein and defensin mRNA levels and reduced viral replication during parainfluenza virus type 3 pneumonia in neonatal lambs. Clin Diagn Lab Immunol 2004; 11(3):599-607.
7. Applied Biosystems User Bulletin #2, ABI PRISM 7700 Sequence Detection System. SUBJECT: Relative Quantitation of Gene Expression: "Calculating the Input Amount." 2001. p. 8.
8. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29(9):e45.
9. Bustin SA. A-Z of Quantitative PCR. Bustin SA, editor. (IUL Biotechnology, No. 5), 1st ed. International University Line; August 2004. 882 pages.
10. Mocellin S, Rossi CR, Pilati P, Nitti D, Marincola FM. Quantitative real-time PCR: a powerful ally in cancer research. Trends Mol Med 2003; 9(5):189-195.
11. Wang E, Miller LD, Ohnmacht GA, Liu ET, Marincola FM. High-fidelity mRNA amplification for gene profiling. Nat Biotechnol 2000; 18(4):457-459.
12. Gallup JM, Grubor B, Barua AB, Mohammadi G, Brogden KA, Olson JA, Mark R, Ackermann MR. Repeated intravenous doses of all-trans-retinoyl β-D-glucuronide is not effective in the treatment of bacterial bronchopneumonia in lambs but is devoid of gross and acute toxicity. Med Sci Monit 2002; 8(9):BR345-BR353.
13. Caverly JM, Diamond G, Gallup JM, Brogden KA, Dixon RAF, Ackermann MR. Coordinated Expression of Tracheal Antimicrobial Peptide and Inflammatory Response Elements in the Lungs of Neonatal Calves and with Acute Bacterial Pneumonia. Inf Immun 2003; 71(5):2950-2955.
14. Opriessnig T, Yu S, Gallup JM, Evans RB, Fenaux M, Pallares FJ, Thacker EL, Brockus CW, Ackermann MR, Thomas P, Meng XJ, Halbur PG. Effect of Vaccination with selective Bacterins on conventional Pigs infected with Type 2 Porcine Circovirus. Vet Pathol 2003; 40(6):521-529.
15. Opriessnig T, Fenaux M, Yu S, Evans RB, Cavanaugh D, Gallup JM, Ackermann MR, Pallares FJ, Thacker EL, Lager KM, Meng XJ, Halbur PG. Effect of Porcine Parvovirus Vaccination on the Development of PMWS in Segregated Early Weaned Pigs Coinfected with Type 2 Porcine Circovirus and Porcine Parvovirus. Veterinary Microbiology 2004; 98(3-4):209-220.
16. Meyerholz DK, Gallup JM, Grubor BM, Evans RB, Tack BF, McCray Jr. PB, Ackermann MR. Developmental expression and distribution of sheep b-defensin-2. Dev Comp Immun 2004; 28:171-178.
17. Grubor BM, Gallup JM, Meyerholz DK, Crouch EC, Evans RB, Brogden KA, Lehmkuhl HD, Ackermann MR. Enhanced Surfactant Protein and Defensin mRNA Levels and Reduced Viral Replication during Parainfluenza Virus Type 3 Pneumonia in Neonatal Lambs. CDLI 2004; 11(3):599-607.
18. Ackermann MR, Gallup JM, Zabner J, Evans RB, Brockus CW, Meyerholz DK, Grubor B, Brogden KA. Differential expression of sheep beta-defensin-1 and -2 and interleukin 8 during acute Mannheimia haemolytica pneumonia. Microbial Pathogenesis 2004; 37:21-27.
19. Grubor B, Gallup JM, Ramírez-Romero R, Bailey TB, Crouch EC, Brogden KA, Ackermann MR. Surfactant Protein D expression in normal and pneumonic ovine lung. Vet Immunology & Immunopathology 2004; 101:235-242.
20. Meyerholz DK, Grubor B, Fach SJ, Sacco RE, Lehmkuhl HD, Gallup JM, Ackermann MR. Reduced Clearance of Respiratory Syncytial Virus in a Preterm Lamb Model. Microbes & Infection 2004; 6(14):1312-1319.
21. Meyerholz DK, Grubor B, Gallup JM, Lehmkuhl HD, Anderson RD, Lazic T, Ackermann MR. Adenovirus-Mediated Gene Therapy Enhances Parainfluenza Virus 3 Infection in Neonatal Lambs. J Clin Micro 2004; 42(10):4780-4787.
22. Broackes-Carter F, Mouchel N, Gill D, Hyde S, Bassett J, Harris A. Quantitative CFTR transcript analysis by TaqMan. Temporal regulation of CFTR expression during ovine lung development: implications for CF gene therapy. Hum Mol Genet 2002; 11:125-131.
23. Swillens S, Goffard J-C, Marechal Y, de Kerchove d'Exaerde A, Housni EH. Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve. Nuc Acids Res 2004; 32(6):e53.
24. Peirson SN, Butler JN, Foster RG. Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nuc Acids Res 2003; 31(14):e73.