Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry

BMC Research Notes

Volumn 4, Issue , 2011, Pages

  Ponomarev, Eugene D ^a   Veremeyko, Tatiana ^a   Barteneva, Natasha S ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

CDK6; Imaging cytometry; MicroRNA; MiR 124; Neuroblastoma; Target gene

Indexed keywords

EID: 82155187070     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-4-517     Document Type: Article

References (19)

1. MicroRNAs: synthesis, mechanism, function, and recent clinical trials. Wahid F, Shehzad A, Khan T, Kim YY, Biochim Biophys Acta 2010 1803 1231 1243 10.1016/j.bbamcr.2010.06.013 20619301
2. MicroRNA regulation of neural stem cells and neurogenesis. Shi Y, Zhao X, Hsieh J, Wichterle H, Impey S, Banerjee S, Neveu P, Kosik KS, J Neurosci 2010 30 14931 14936 10.1523/JNEUROSCI.4280-10.2010 21068294
3. MicroRNAs as regulators of differentiation and cell fate decisions. Ivey KN, Srivastava D, Cell Stem Cell 2010 7 36 41 10.1016/j.stem.2010.06.012 20621048
4. The microRNA pathway and cancer. Kwak PB, Iwasaki S, Tomari Y, Cancer Sci 2010 101 2309 2315 10.1111/j.1349-7006.2010.01683.x 20726859
5. MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-alpha-PU.1 pathway. Ponomarev ED, Veremeyko T, Barteneva N, Krichevsky AM, Weiner HL, Nat Med 2011 17 64 70 10.1038/nm.2266 21131957
6. MicroRNAs and their therapeutic potential for human diseases: aberrant microRNA expression in Alzheimer's disease brains. Satoh J, J Pharmacol Sci 2010 114 269 275 10.1254/jphs.10R11FM 20953120
7. Quantitation of microRNAs by real-time RT-qPCR. Chen C, Tan R, Wong L, Fekete R, Halsey J, Methods Mol Biol 2011 687 113 134 10.1007/978-1-60761-944-4- 8 20967604
8. In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and cell preparations. Nuovo GJ, Methods 2008 44 39 46 10.1016/j.ymeth.2007.10.008 18158131 (Pubitemid 350297988)
9. Expanding applications of protein analysis using proximity ligation and qPCR. Swartzman E, Shannon M, Lieu P, Chen SM, Mooney C, Wei E, Kuykendall J, Tan R, Settineri T, Egry L, Ruff D, Methods 2010 50 23 S26 10.1016/j.ymeth.2010. 01.024 20215017
10. Cellular context in epigenetics: quantitative multicolor imaging and automated per-cell analysis of miRNAs and their putative targets. Mansfield JR, Methods 2010 52 271 280 10.1016/j.ymeth.2010.10.001 20933088
11. Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology. Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA, PLoS One 2010 5 9625 10.1371/journal.pone.0009625 20224781
12. Chemogenomics and parasitology: small molecules and cell-based assays to study infectious processes. Muskavitch MA, Barteneva N, Gubbels MJ, Comb Chem High Throughput Screen 2008 11 624 646 10.2174/138620708785739989 18795882
13. Number matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry. Ahmed F, Friend S, George TC, Barteneva N, Lieberman J, J Immunol Methods 2009 347 79 86 10.1016/j.jim.2009.05.014 19524586
14. The ImageStream System: a key step to a new era in imaging. Zuba-Surma EK, Kucia M, Abdel-latif A, Lillard JW Jr, Ratajczak MZ, Folia Histochem Cytobiol 2007 45 279 290 18165167 (Pubitemid 351193602)
15. Context-dependent functions of specific microRNAs in neuronal development. Gao FB, Neural Dev 2010 5 25 10.1186/1749-8104-5-25 20920300
16. Regulation of cyclin dependent kinase 6 by microRNA 124 in medulloblastoma. Pierson J, Hostager B, Fan R, Vibhakar R, J Neurooncol 2008 90 1 7 10.1007/s11060-008-9624-3 18607543
17. Cycling at the interface between neurodevelopment and neurodegeneration. Nguyen MD, Mushynski WE, Julien JP, Cell Death Differ 2002 9 1294 1306 10.1038/sj.cdd.4401108 12478466 (Pubitemid 36097699)
18. A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets. Nuovo GJ, Elton TS, Nana-Sinkam P, Volinia S, Croce CM, Schmittgen TD, Nat Protoc 2009 4 107 115 10.1038/nprot.2008.215 19131963
19. Pitfalls in diagnostic immunohistology. Leong AS, Adv Anat Pathol 2004 11 86 93 10.1097/00125480-200403000-00002 15090844