A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets

Nature Protocols

Volumn 4, Issue 1, 2009, Pages 107-115

  Nuovo, Gerard J ^a   Elton, Terry S ^a   Nana Sinkam, Patrick ^a   Volinia, Stefano ^a,b   Croce, Carlo M ^a,b   Schmittgen, Thomas D ^a  

^a THE OHIO STATE UNIVERSITY WEXNER MEDICAL CENTER   (United States)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE; MICRORNA; PROTEINASE;

ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; DNA PROBE; EMBRYO; ENZYME METABOLISM; GENE AMPLIFICATION; GENE EXPRESSION; GENE IDENTIFICATION; GENE TARGETING; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA HYBRIDIZATION; SIGNAL TRANSDUCTION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY;

IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MICRORNAS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 61449254438     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.215     Document Type: Article

References (19)

1. Nuovo, G.J. In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and cell preparations. Methods 44, 39-46 (2008).
2. Lee, E.J. et al. Classification of microRNA processing patterns in tissues, cell lines, and tumors. RNA 14, 35-42 (2008).
3. Calin, G.A. & Croce, C.M. MicroRNA signatures in human cancers. Nat. Rev. Cancer 6, 857-866 (2006).
4. Garzon, R., Fabbri, M., Cimmino, A., Calin, G.A. & Croce, C.M. MicroRNA expression and function in cancer. Trends Mol. Med. 12, 580-587 (2006).
5. Yanaihara, N. et al. Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell 9, 189-198 (2006).
6. Gramantieri, L. et al. Cyclin G1 is a target of miR-122a, a microRNA frequently down-regulated in human hepatocellular carcinoma. Cancer Res. 67, 6092-6099 (2007).
7. Cimmino, A. et al. miR-15 and miR-16 induce apoptosis by targeting bcl-2. Proc. Natl. Acad. Sci. USA 102, 13944-13949 (2005).
8. Kuhn, D.E. et al. Experimental validation of miR targets. Methods 44, 47-54 (2008).
9. Nuovo, G.J. Co labeling using RT in situ PCR: A review. J. Histochem. Cytochem. 49, 1329-1339 (2001).
10. Nuovo, G.J. PCR In Situ Hybridization: Protocols and Applications 3rd edn. (Lippincott, Williams and Wilkins, Raven Press, New York, 1996).
11. Politz, J.C., Zhang, F. & Pederson, T. MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cells. Proc. Natl. Acad. Sci. USA 103, 18957-18962 (2006).
12. Obernosterer, G., Martinez, J. & Alenius, M. Locked nucleic acid based in situ detection of microRNAs in mouse tissue sections. Nat. Protoc. 2, 1508-1514 (2007).
13. Sempere, L.F. et al. Altered MicroRNA expression confined to specific epithelial cell subpopulations in breast cancer. Cancer Res. 67, 11612-11620 (2007).
14. Schetter, A.J. et al. MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA 299, 425-436 (2008).
15. Silahtaroglu, A.N. et al. Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification. Nat. Protoc. 2, 2520-2528 (2007).
16. Bak, M. et al. MicroRNA expression in the adult mouse nervous system. RNA 14, 432-444 (2008).
17. Jiang, J., Lee, E.J., Gusev, Y. & Schmittgen, T.D. Real-time expression profiling of microRNA precursors in human cancer cell lines. Nucleic Acids Res. 33, 5394-5403 (2005).
18. Schmittgen, T.D., Jiang, J., Liu, Q. & Yang, L. A high-throughput method to monitor the expression of microRNA precursors. Nucleic Acids Res. 32, 43-47 (2004).
19. Liang, Y., Ridzon, D., Wong, L. & Chen, C. Characterization of microRNA expression profiles in normal human tissues. BMC Genomics 8, 166 (2007).