Numbers matter: Quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry

Journal of Immunological Methods

Volumn 347, Issue 1-2, 2009, Pages 79-86

  Ahmed, Fariyal ^a   Friend, Sherree ^b   George, Thaddeus C ^b   Barteneva, Natasha ^a   Lieberman, Judy ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b Amnis Corporation   (United States)

Author keywords

Flow cytometry; Fluorescence microscopy; Imaging flow cytometry; Immunological synapse

Indexed keywords

CD3 ANTIGEN; PROTEIN KINASE LCK;

ANTIGEN PRESENTING CELL; ARTICLE; CD3+ T LYMPHOCYTE; CELL JUNCTION; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; IMMUNOLOGICAL SYNAPSE; MACROPHAGE; MOLECULAR DYNAMICS; MOLECULAR IMAGING; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; T LYMPHOCYTE ACTIVATION;

ANTIGEN-PRESENTING CELLS; ANTIGENS, CD3; CD8-POSITIVE T-LYMPHOCYTES; CELLS, CULTURED; COCULTURE TECHNIQUES; FLOW CYTOMETRY; HUMANS; IMAGE CYTOMETRY; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOLOGICAL SYNAPSES; KINETICS; LYMPHOCYTE ACTIVATION; LYMPHOCYTE SPECIFIC PROTEIN TYROSINE KINASE P56(LCK); MACROPHAGES; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION;

EID: 67650495486     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.05.014     Document Type: Article

References (37)

1. Anikeeva N., Somersalo K., Sims T.N., Thomas V.K., Dustin M.L., and Sykulev Y. Distinct role of lymphocyte function-associated antigen-1 in mediating effective cytolytic activity by cytotoxic T lymphocytes. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 6437
2. Beal A.M., Anikeeva N., Varma R., Cameron T.O., Norris P.J., Dustin M.L., and Sykulev Y. Protein Kinase Cθ regulates stability of the peripheral adhesion ring junction and contributes to the sensitivity of target cell lysis by CTL. J. Immunol. 181 (2008) 4815
3. Bossi G., and Griffiths G.M. CTL secretory lysosomes: biogenesis and secretion of a harmful organelle. Semin. Immunol. 17 (2005) 87
4. Brossard C., Feuillet V., Schmitt A., Randriamampita C., Romao M., Raposo G., and Trautmann A. Multifocal structure of the T cell-dendritic cell synapse. Eur. J. Immunol. 35 (2005) 1741
5. Bunnell S.C., Hong D.I., Kardon J.R., Yamazaki T., McGlade C.J., Barr V.A., and Samelson L.E. T cell receptor ligation induces the formation of dynamically regulated signaling assemblies. J. Cell Biol. 158 (2002) 1263
6. Burbach B.J., Srivastava R., Medeiros R.B., O'Gorman W.E., Peterson E.J., and Shimizu Y. Distinct regulation of integrin-dependent T cell conjugate formation and NF-kB activation by the adaptor protein ADAP. J. Immunol. 181 (2008) 4840
7. Cemerski S., and Shaw A. Immune synapses in T-cell activation. Curr. Opin. Immunol. 18 (2006) 298
8. Crocenzi T.S., Tretter C.P.G., Schwaab T., Schned A.R., Heaney J.A., Cole B.F., Fisher J.L., and Ernstoff M.S. Impaired cytolytic activity in peripheral blood T cells from renal cell carcinoma patients. Clin. Immunol. 117 (2005) 6
9. Dustin M.L. The cellular context of T cell signaling. Immunity 30 (2009) 482
10. Ehrlich L.I.R., Ebert P.J.R., Krummel M.F., Weiss A., and Davis M.M. Dynamics of p56Lck translocation to the T cell immunological synapse following agonist and antagonist stimulation. Immunity 17 (2002) 809
11. Gati A., Guerra N., Giron-Michel J., Azzarone B., Angevin E., Moretta A., Chouaib S., and Caignard A. Tumor cells regulate the lytic activity of tumor-specific cytotoxic T lymphocytes by modulating the inhibitory natural killer receptor function. Cancer Res. 61 (2001) 3240
12. George T.C., Fanning S.L., Fitzgeral-Bocarsly P., Medeiros R.B., Highfill S., Shimizu Y., Hall B.E., Frost K., Basiji D., Ortyn W.E., Morrissey P.J., and Lynch D.H. Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow. J. Immunol. Methods 311 (2006) 117
13. George T.C., Morrissey P.J., Cui C., Singh S., and Bocarsly P.F. Measurement of cytoplasmic to nuclear translocation. In: Robinson J.P. (Ed). Current Protocols in Cytometry, Vol. Supplement 47 (2009), John Wiley and Sons, Inc., New York 9.28.1
14. Grakoui A., Bromley S.K., Sumen C., Davis M.M., Shaw A.S., Allen P.M., and Dustin M.L. The immunological synapse: a molecular machine controlling T cell activation. Science 285 (1999) 221
15. Groves J.T., and Dustin M.L. Supported planar bilayers in studies on immune cell adhesion and communication. J. Immunol. Methods 278 (2003) 19
16. Holdorf A.D., Lee K.-H., Burack R., Allen P.M., and Shaw A.S. Regulation of Lck activity by CD4 and CD28 in the immunological synapse. Nat. Immunol. 3 (2002) 259
17. Huse M., Lillemeier B.F., Kuhns M.S., Chen D.S., and Davis M.M. T cells use two directionally distinct pathways for cytokine secretion. Nat. Immunol. 7 (2006) 247
18. Iwashima M., Irving B.A., van Oers N.S., and Weiss A. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science 263 (1994) 1136
19. Kane L.P., Lin J., and Weiss A. Signal transduction by the TCR for antigen. Curr. Opin. Immunol. 12 (2000) 242
20. Makedonas G., Banerjee P.P., Pandey R., Hersperger A.R., Sanborn K.B., Gareth A.D.H., Orange J.S., and Betts M.R. Rapid up-regulation and granule-independent transport of perforin to the immunological synapse define a novel mechanism of antigen-specific CD8+ T cell cytotoxic activity. J. Immunol. 182 (2009) 5560
21. Maldonado R.A., Irvine D.J., Schreiber R., and Glimcher L.H. A role for the immunological synapse in lineage commitment of CD4 lymphocytes. Nature 431 (2004) 527
22. Maxwell S.E., Kelley K., and Rausch J.R. Sample size planning for statistical power and accuracy in parameter estimation. Ann. Rev. Psychol. 59 (2008) 537
23. Medeiros R.B., Burbach B.J., Mueller K.L., Srivastava R., Moon J.J., Highfill S., Peterson E.J., and Shimizu Y. Regulation of NF-kB activation in T cells via association of the adaptor proteins ADAP and CARMA1. Science 316 (2007) 754
24. Monks C.R., Freiberg H., Sciaky N., and Kupfer A. Three-dimensional segregation of supramolecular activation clusters in T cells. Nature 395 (1998) 82
25. Mossman K.D., Campi G., Groves J.T., and Dustin M.L. Altered TCR signaling from geometrically repatterned immunological synapses. Science 310 (2005) 1191
26. Potter T.A., Grebe K., Freiberg B., and Kupfer A. Formation of supramolecular activation clusters on fresh ex vivo CD8+ T cells after engagement of the T cell antigen receptor and CD8 by antigen-presenting cells. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 12624
27. Saito T., and Yokosuka T. Immunological synapse and microclusters: the site for recognition and activation of T cells. Curr. Opin. Immunol. 18 (2006) 305
28. Saverino D., Tenca C., Zarcone D., Merlo A., Megiovanni A.M., Valle M.T., Manca F., Grossi C.E., and Ciccone E. CTLA-4 (CD152) inhibits the specific lysis mediated by human cytolytic T lymphocytes in a clonally distributed fashion. J. Immunol. 162 (1999) 651
29. Seed B., and Xavier R. Spinophilin and the immune synapse. J. Cell Biol. 181 (2008) 181
30. Serrador J.M., Cabrero J.R., Sancho D., Mittelbrunn M., Urzainqui A., and Sanchez-Madrid F. HDAC6 deacetylase activity links the tubulin cytoskeleton with immune synapse organization. Immunity 20 (2004) 417
31. Sims T.N., Soos T.J., Xenias H.S., Thaler -D. B., Hofman J.M., Waite J.C., Cameron T.O., Thomas V.K., Varma R., Wiggins C.H., Sheetz M.P., Littman D.R., and Michael L.D. Opposing effects of PKCθ and WASp on symmetry breaking and relocation of the immunological synapse. Cell 129 (2007) 773
32. Somersalo K., Anikeeva N., Sims T.N., Thomas V.K., Strong R.K., Spies T., Lebedeva T., Sykulev Y., and Dustin M.L. Cytotoxic T lymphocytes form an antigen-independent ring junction. J. Clin. Invest. 113 (2004) 49
33. Stinchcombe J.C., Bossi G., Booth S., and Griffiths G.M. The immunological synapse of CTL contains a secretory domain and membrane bridges. Immunity 15 (2001) 751
34. Stinchcombe J.C., Majorovits E., Bossi G., Fuller S., and Griffiths G.M. Centrosome polarization delivers secretory granules to the immunological synapse. Nature 443 (2006) 462
35. Wulfing C., Purtic B., Klem J., and Schatzle J.D. Stepwise cytoskeletal polarization as a series of checkpoints in innate but not adaptive cytolytic killing. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 7767
36. Yeh J.-H., Sidhu S.S., and Chan A.C. Regulation of a late phase of T cell polarity and effector functions by Crtam. Cell 132 (2008) 846
37. Yokosuka T., Sakata-Sogawa K., Kobayashi W., Hiroshima M., Hashimoto-T. A., Tokunaga M., Dustin M.L., and Saito T. Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of ZAP70 and SLP-76. Nat. Immunol. 6 (2005) 1253