Evaluation of novel high-throughput embryonic stem cell tests with new molecular markers for screening embryotoxic chemicals in vitro

Toxicological Sciences

Volumn 124, Issue 2, 2011, Pages 460-471

  Suzuki, Noriyuki ^a   Ando, Satoshi ^a   Yamashita, Norihisa ^a   Horie, Nobuyuki ^a   Saito, Koichi ^a  

^a SUMITOMO CHEMICAL CO LTD   (Japan)

Author keywords

Differentiation; Embryonic stem cell test; Embryonic stem cells; Embryotoxicity; In vitro; Reporter gene assay

Indexed keywords

CARDIOMYOPATHY ASSOCIATED 1; HEART AND NEURAL CREST DERIVATIVE EXPRESSED TRANSCRIPT 1; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; UPSTREAM STIMULATORY FACTOR;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; EMBRYONIC STEM CELL; EMBRYOTOXICITY; GENE EXPRESSION; HEART DEVELOPMENT; HEART MUSCLE CELL; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MORPHOGENESIS; MOUSE; NEURAL CREST; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTIVE VALUE; REPORTER GENE;

3T3 CELLS; ANIMAL TESTING ALTERNATIVES; ANIMALS; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; CELL SURVIVAL; DNA-BINDING PROTEINS; EMBRYONIC STEM CELLS; GENE EXPRESSION; HIGH-THROUGHPUT SCREENING ASSAYS; LUCIFERASES; MICE; MYOCYTES, CARDIAC; NUCLEAR PROTEINS; PLASMIDS; PROMOTER REGIONS, GENETIC; TERATOGENS; TOXICITY TESTS; TRANSGENES;

EID: 81855211066     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfr250     Document Type: Article

References (35)

1. Bremer, S., and Hartung, T. (2004). The use of embryonic stem cells for regulatory developmental toxicity testing in vitro-the current status of test development. Curr. Pharm. Des. 10, 2733-2747.
2. Bremer, S., Worth, A. P., Paparella, M., Bigot, K., Kolossov, E., Fleischmann, B. K., Hescheler, J., and Balls, M. (2001). Establishment of an in vitro reporter gene assay for developmental cardiac toxicity. Toxicol. In Vitro 15, 215-223.
3. Brown, N. A. (2002). Selection of the test chemicals for the ECVAM international validation study on in vitro embryotoxicity tests. Altern. Lab. Anim. 30, 177-198.
4. Buesen, R., Genschow, E., Slawik, B., Visan, A., Spielmann, H., Luch, A., and Seiler, A. (2009). Embryonic stem cell test remastered: Comparison between the validated EST and the new molecular FACS-EST for assessing developmental toxicity in vitro. Toxicol. Sci. 108, 389-400.
5. Chen, R., Chen, J., Cheng, S., Qin, J., Li, W., Zhang, L., Jiao, H., Yu, X., Zhang, X., Lahn, B. T., et al. (2010). Assessment of embryotoxicity of compounds in cosmetics by the embryonic stem cell test. Toxicol. Mech. Methods 20, 112-118.
6. Cross, J. C., Flannery, M. L., Blanar, M. A., Steingrimsson, E., Jenkins, N. A., Copeland, N. G., Rutter, W. J., and Werb, Z. (1995). Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development. Development 121, 2513-2523.
7. de Jong, E., Louisse, J., Verwei, M., Blaauboer, B. J., van de Sandt, J. J. M., Woutersen, R. A., Rietjens, I. M. C. M., and Piersma, A. H. (2009). Relative developmental toxicity of glycol ether alkoxy acid metabolites in the embryonic stem cell test as compared with the in vivo potency of their parent compounds. Toxicol. Sci. 110, 117-124.
8. di Guglielmo, C., Lopez, D. R., de Lapuente, J., Mallafre, J. M. L., and Suarez, M. B. (2010). Embryotoxicity of cobalt ferrite and gold nanoparticles: A first in vitro approach. Reprod. Toxicol. 30, 271-276.
9. Eckardt, K., and Stahlmann, R. (2010). Use of two validated in vitro tests to assess the embryotoxic potential of mycophenolic acid. Arch. Toxicol. 84, 37-43.
10. Festag, M., Vierte, I. B., Steinberg, P., and Sehner, C. (2007). An in vitro embryotoxicity assay based on the disturbance of the differentiation of murine embryonic stem cells into endothelial cells. II. Testing of compounds. Toxicol. In Vitro 21, 1631-1640.
11. Genschow, E., Spielmann, H., Scholz, G., Pohl, I., Seiler, A., Clemann, N., Bremer, S., and Becker, K. (2004). Validation of the embryonic stem cell test (EST) in the ECVAM international validation study on in vitro embryotoxicity. Altern. Lab. Anim. 32, 209-244.
12. Gustafson-Wagner, E. A., Sinn, H. W., Chen, Y. L., Wang, D. Z., Reiter, R. S., Lin, J. L., Yang, B., Williamson, R. A., Chen, J., Lin, C. I., et al. (2007). Loss of mXin alpha, an intercalated disk protein, results in cardiac hypertrophy and cardiomyopathy with conduction defects. Am. J. Physiol. Heart Circ. Physiol. 293, 2680-2692.
13. Hartung, T. (2009). Chemical regulators have overreached. Nature 460, 1080-1081.
14. OECD. (2009). OECD Guidelines for the Testing of Chemicals. No. 455: The Stably Transfected Human Estrogen Receptor-Alpha Transcriptional Activation Assay for Detection of Estrogenic Agonist-Activity of Chemicals. Organisation for Economic Cooperation and Development, Paris, France.
15. Ooe, N., Kobayashi, K., Motonaga, K., Saito, K., and Kaneko, H. (2009). Characterization of functional heterodimer partners in brain for a bHLH-PAS factor NXF. Biochem. Biophys. Res. Commun. 378, 761-765.
16. Paquette, J. A., Kumpf, S. W., Streck, R. D., Thomson, J. J., Chapin, R. E., and Stedman, D. B. (2008). Assessment of the embryonic stem cell test and application and use in the pharmaceutical industry. Birth Defects Res. B Dev. Reprod. Toxicol. 283, 104-111.
17. Pellizzer, C., Adler, S., Corvi, R., Hartung, T., and Bremer, S. (2004). Monitoring of teratogenic effects in vitro by analysing a selected gene expression pattern. Toxicol. In Vitro 18, 325-335.
18. Peters, A. K., Steemans, M., Mesens, N., Hansen, E., Verheyen, G. R., and Vanparys, P. (2008). Evaluation of the embryotoxic potency of compounds in a newly revised embryonic stem cell test. Toxicol. Sci. 105, 342-350.
19. Saito, K., Tomigahara, Y., Ohe, N., Isobe, N., Nakatsuka, I., and Kaneko, H. (2000). Lack of significant estrogenic or antiestrogenic activity of pyrethroid insecticides in three in vitro assays based on classic estrogen receptor alphamediated mechanisms. Toxicol. Sci. 57, 54-60.
20. Scholz, G., Pohl, I., Genschow, E., Klemm, M., and Spielmann, H. (1999). Embryotoxicity screening using embryonic stem cells in vitro: Correlation to in vivo teratogenicity. Cells Tissues Organs 165, 203-211.
21. Seiler, A., Visan, A., Buesen, R., Genschow, E., and Spielmann, H. (2004). Improvement of an in vitro stem cell assay for developmental toxicity: The use of molecular endpoints in the embryonic stem cell test. Reprod. Toxicol. 18, 231-240.
22. Seiler, A., Buesen, R., Visan, A., and Spielmann, H. (2006). Use of murine embryonic stem cells in embryotoxicity assays: The embryonic stem cell test. Methods Mol. Biol. 329, 371-395.
23. Soprano, D. R., Teets, B. W., and Soprano, K. J. (2007). Role of retinoic acid in the differentiation of embryonal carcinoma and embryonic stem cells. Vitam. Horm. 75, 69-95.
24. Spielmann, H., Pohl, I., Döring, B., Liebsch, M., and Moldenhauer, F. (1997). The embryonic stem cell test (EST), an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. Toxicol. In Vitro 10, 119-127.
25. Spielmann, H. (2009). The way forward in reproductive/developmental toxicity testing. Altern. Lab. Anim. 37, 641-656.
26. Spielmann, H., and Vogel, R. (2006). REACH testing requirements must not be driven by reproductive toxicity testing in animals. Altern. Lab. Anim. 34, 365-366.
27. Stummann, T. C., and Bremer, L. H. S. (2008). Embryotoxicity hazard assessment of cadmium and arsenic compounds using embryonic stem cells. Toxicology 252, 118-122.
28. Sumida, K., and Saito, K. (2008). Molecular cloning of estrogen receptors from fathead minnow (Pimephales promelas) and bluegill (Lepomis macrochirus) fish: Limited piscine variation in estrogen receptor-mediated reporter gene transactivation by xenoestrogens. Environ. Toxicol. Chem. 27, 489-498.
29. Sumida, K., Ooe, N., Saito, K., and Kaneko, H. (2003). Limited species differences in estrogen receptor alpha-medicated reporter gene transactivation by xenoestrogens. J. Steroid Biochem. Mol. Biol. 84, 33-40.
30. Suzuki, N., Ando, S., Horie, N., Sumida, K., and Saito, K. (2011). Analysis of altered gene expression specific to embryotoxic chemical treatment during embryonic stem cell differentiation into myocardiac and neural cells. J. Toxicol. Sci. 36, 569-585.
31. Uibel, F., Muhleisen, A., Kohle, C., Weimer, M., Stummann, T. C., Bremer, S., and Schwarz, M. (2010). ReProGlo: A new stem cell-based reporter assay aimed to predict embryotoxic potential of drugs and chemicals. Reprod. Toxicol. 30, 103-112.
32. van Dartel, D. A. M., Zeijen, N. J. L., de la Fonteyne, L. J. J., van Schooten, F. J., and Piersma, A. H. (2009). Disentangling cellular proliferation and differentiation in the embryonicstem cell test, and its impact on the experimental protocol. Reprod. Toxicol. 28, 254-261.
33. Wang, D. Z., Reiter, R. S., Lin, J. L., Wang, Q., Williams, H. S., Krob, S. L., Schultheiss, T. M., Evans, S., and Lin, J. J. (1999). Requirement of a novel gene, Xin, in cardiac morphogenesis. Development 126, 1281-1294.
34. zur Nieden, N. I., Ruf, L. J., Kempka, G., Hildebrand, H., and Ahr, H. J. (2001). Molecular markers in embryonic stem cells. Toxicol. In Vitro 15, 455-461.
35. zur Nieden, N. I., Kempka, G., and Ahr, H. J. (2004). Molecular multiple endpoint embryonic stem cell test-A possible approach to test for the teratogenic potential of compounds. Toxicol. Appl. Pharmacol. 194, 257-269.