An improved protocol for DNA extraction from alkaline soil and sediment samples for constructing metagenomic libraries

Applied Biochemistry and Biotechnology

Volumn 165, Issue 2, 2011, Pages 454-464

  Verma, Digvijay ^a   Satyanarayana, T ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Humic substances; Metagenomic DNA; Polyethylene glycol 8000; Polyvinylpolypyrrolidone; Powdered activated charcoal

Indexed keywords

ALKALINE SOILS; CELL LYSIS; DNA EXTRACTION; HUMIC SUBSTANCES; ISO-PROPANOLS; ISOLATION AND PURIFICATION; METAGENOMIC DNA; METAGENOMIC LIBRARIES; PCR AMPLIFICATION; POLYVINYLPOLYPYRROLIDONE; PURITY LEVEL; RESTRICTION DIGESTION; SINGLE-STEP;

ACTIVATED CARBON; ANOXIC SEDIMENTS; DNA; GLYCOLS; LIBRARIES; PHOTODEGRADATION; POLYETHYLENE GLYCOLS; SEDIMENTOLOGY; SOILS;

POLYMERASE CHAIN REACTION;

2 PROPANOL; ACTIVATED CARBON; MACROGOL;

ARTICLE; CONTROLLED STUDY; CYTOLYSIS; DNA EXTRACTION; DNA ISOLATION; DNA PURIFICATION; HUMIC SUBSTANCE; METAGENOMICS; PRECIPITATION; SEDIMENT; SOIL;

2-PROPANOL; ARCHAEA; BACTERIA; CELL EXTRACTS; CHARCOAL; DNA, ARCHAEAL; DNA, BACTERIAL; DNA, FUNGAL; DNA, INTERGENIC; FUNGI; GENOMIC LIBRARY; METAGENOME; METAGENOMICS; MICROBIAL CONSORTIA; POLYETHYLENE GLYCOLS; POLYMERASE CHAIN REACTION; POVIDONE; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY; XYLANS;

EID: 81455141559     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-011-9264-5     Document Type: Article

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