Enzymatic analysis of an amylolytic enzyme from the hyperthermophilic archaeon Pyrococcus furiosus reveals its novel catalytic properties as both an α-amylase and a cyclodextrin-hydrolyzing enzyme

Applied and Environmental Microbiology

Volumn 70, Issue 10, 2004, Pages 5988-5995

  Yang, Sung Jae ^a   Lee, Hee Seob ^a   Park, Cheon Seok ^b   Kim, Yong Ro ^a   Moon, Tae Wha ^a   Park, Kwan Hwa ^a  

^a Seoul National University   (South Korea)

^b Kyung Hee University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CLONING; ENZYMES; ESCHERICHIA COLI; HYDROLYSIS;

CATALYTIC PROPERTIES; ENZYMATIC ANALYSIS;

MICROBIOLOGY;

ACARBOSE; ALPHA CYCLODEXTRIN; AMYLASE; AMYLASE INHIBITOR; BACTERIAL DNA; BETA CYCLODEXTRIN; CYCLODEXTRIN HYDROLYZING ENZYME; GAMMA CYCLODEXTRIN; GLYCOSIDASE; MALTOHEPTAOSE; MALTOSE; MALTOTETROSE; MALTOTRIOSE; OLIGOSACCHARIDE; PANOSE; PROTEIN PFTA; PULLULAN; PULLULANASE; RECOMBINANT ENZYME; STARCH; TETROSE; UNCLASSIFIED DRUG;

ENZYME;

ARCHAEBACTERIUM; ARTICLE; CATALYSIS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ANALYSIS; ENZYME ASSAY; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PROTEIN EXPRESSION; PYROCOCCUS FURIOSUS; TEMPERATURE; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CARBOHYDRATE SEQUENCE; CYCLODEXTRINS; DNA, ARCHAEAL; ENZYME STABILITY; ESCHERICHIA COLI; GENES, ARCHAEAL; HEAT; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PYROCOCCUS FURIOSUS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ARCHAEA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; PYROCOCCUS; PYROCOCCUS FURIOSUS;

EID: 8144230220     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.10.5988-5995.2004     Document Type: Article

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