Validation and application of reference genes for quantitative gene expression analyses in various tissues of Bupleurum chinense

Molecular Biology Reports

Volumn 38, Issue 8, 2011, Pages 5017-5023

  Dong, Lemeng ^a,b   Sui, Chun ^a   Liu, Yujun ^b   Yang, Yun ^a   Wei, Jianhe ^a   Yang, Yanfang ^a  

^a INSTITUTE OF MEDICINAL PLANT DEVELOPMENT   (China)

^b BEIJING FORESTRY UNIVERSITY   (China)

Author keywords

AS; Bupleurum chinense DC.; FPS; HMGR; IPPI; Quantitative real time PCR; Reference gene

Indexed keywords

SAIKOSAPONIN;

18S RIBOSOMAL RNA GENE; 3 HYDROXY 3 METHYLGLUTARYL COENZYME A REDUCTASE GENE; ACTIN GENE; ALPHA TUBULIN GENE; ARTICLE; BETA AMYRIN SYNTHASE GENE; BETA TUBULIN GENE; BIOSYNTHESIS; BUPLEURUM CHINENSE; CONTROLLED STUDY; CYCLOPHILIN GENE; CYTOPLASMIC RIBOSOMAL PROTEIN GENE; ELONGATION FACTOR 1 ALPHA GENE; FARNESYL PYROPHOSPHATE SYNTHASE GENE; FLOWER; FRUIT; GENE EXPRESSION PROFILING; GENE FUNCTION; GENE SEQUENCE; ISOPENTENYL DIPHOSPHATE ISOMERASE GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENE; PLANT LEAF; PLANT ROOT; PLANT STEM; PLANT TISSUE; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; VALIDATION PROCESS;

BUPLEURUM; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; ORGAN SPECIFICITY; PLANT PROTEINS; POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SOFTWARE; TRANSCRIPTION, GENETIC;

BUPLEURUM CHINENSE;

EID: 81355134083     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-010-0648-3     Document Type: Article

References (42)

1. Jian B, Liu B, Bi YR, Hou WS, Wu CX, Han TF (2008) Validation of internal control for gene expression study in soybean by quantitative real-time PCR. BMC Mol Biol 9:59-72
2. Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A (2004) Guideline to reference gene selection for quantitative realtime PCR. Biochem Biophys Res Commun 313:856-862 (Pubitemid 38050071)
3. Monaco E, Bionaz M, Sobreira de Lima A, Hurley WL, Loor JJ, Wheeler MB (2010) Selection and reliability of internal reference genes for quantitative PCR verification of transcriptomics during the differentiation process of porcine adult mesenchymal stem cells. Stem Cell Res Therapy 1:7
4. Brunner AM, Yakovlev IA, Strauss SH (2004) Validating internal controls for quantitative plant gene expression studies. BMC Plant Biol 4:14
5. Lee PD, Sladek R, Greenwood CMT, Hudson HJ (2001) Control genes and variability: absence ofubiquitous reference transcripts in diverse mammalian expression studies. Genome Res 12:292-297 (Pubitemid 34162995)
6. Stürzenbaum SR, Kille P (2001) Control genes in quantitative molecular biological techniques: the variability of invariance. Comp Biochem Physiol B 130:281-289 (Pubitemid 32913186)
7. Barsalobres-Cavallari CF, Severino FE, Maluf MP, Maia IG (2009) Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions. BMC Mol Biol 10:1
8. Kita T, Hata T, Itoh E, Yoneda R (1980) Analgesic and other pharmacologic actions of saikosaponin in repeated cold stressed (SART stressed) animals. J Pharmacobio-Dyn 3:269-280 (Pubitemid 10016230)
9. Pan SL (ed) (2006) Bupleurum species: scientific evaluation and clinical applications. Taylor & Francis Group, Boca Raton, 272 pp
10. Chinese Pharmacopoeia Commission (2010) Pharmacopoeia of the People's Republic of China, vol I. Medical Publishing House, Beijing, pp 263-264
11. Kim YS, Cho JH, Ahn J, Hwang B (2006) Upregulation of iso-prenoid pathway genes during enhanced saikosaponin biosynthesis in the hairy roots of Bupleurum falcatum. Mol Cells 22(3):269-274 (Pubitemid 46062290)
12. Haralampidis K, Trojanowska M, Osbourn AE (2002) Biosynthesis of triterpenoid saponins in plants. Adv Biochem Eng Biotechnol 75:31-49
13. Ohyama K, Suzuki M, Masuda K, Yoshida S, Muranaka T (2007) Chemical phenotypes of the hmg1 and hmg2 mutants of Ara-bidopsis demonstrate the in-planta role of HMG-CoA reductase in triterpene biosynthesis. Chem Pharm Bull 55:1518-1521 (Pubitemid 350027381)
14. Liao ZH, Chen M, Gong YF, Miao ZQ, Sun XF, Tang KX (2006) Isoprenoid biosynthesis in plants: pathways, genes regulation and metabolic engineering. J Biol Sci 6(1):209-219
15. Okada K, Kasahara H, Yamaguchi S, Kawaide H, Kamiya Y, Nojiri H, Yamane H (2008) Genetic evidence for the role of isopentenyl diphosphate isomerases in the mevalonate pathway and plant development in Arabidopsis. Plant Cell Physiol 49:604-616
16. Kim OT, Kim SH, Ohyama K, Muranaka T, Choi YE, Lee HY, Kim MY, Hwang B (2010) Upregulation of phytosterol and tri-terpene biosynthesis in Centella asiatica hairy roots overex-pressed ginseng farnesyl diphosphate synthase. Plant Cell Rep 29(4):403-411
17. Chen DH, Ye HC, Li GF (2000) Expression of a chimeric far-nesyl diphosphate synthase gene in Artemisia annua L transgenic plants via Agrobacterium tumefaciens-mediated transformation. Plant Sci 155:179-185
18. Ding YX, Ou-Yang X, Shang CH, Ren A, Shi L, YX L, Zhao MW (2008) Molecular cloning, characterization, and differential expression of a farnesyl-diphosphate synthase gene from the Basidiomycetous fungus Ganoderma lucidum. Biosci Biotechnol Biochem 72(6):1571-1579 (Pubitemid 351883788)
19. Daudonnet S, Karst F, Tourte Y (1997) Expression of the farnesyl diphosphate synthase gene of Saccharomyces cerevisiae in tobacco. Mol Breed 3:137-145 (Pubitemid 127507799)
20. Masferrer A, Arró M, Manzano D, Schaller H, Fernández-Bus- quets X, Moncaleán P, Fernández B, Cunillera N, Boronat A, Ferrer A (2002) Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase (FPS1S) in transgenic Arabidopsis induces a cell death/senescence-like response and reduced cytokinin levels. Plant J 30:123-132 (Pubitemid 34536233)
21. Gaffe J, Bru JP, Causse M, Vidal A, Stamitti-Bert L, Carde JP, Gallusci P (2000) LEFPS1, a tomato farnesyl pyrophosphate gene highly expressed during early fruit development. Plant Physiol 123:1351-1362 (Pubitemid 30656693)
22. Suzuki H, Reddy MSS, Naoumkina M, Aziz N, May GD, Huh-man DV, Sumner LW, Blount JW, Mendes P, Dixon RA (2005) Methyl jasmonate and yeast elicitor induce differential tran-scriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula. Planta 220:696-707 (Pubitemid 40393784)
23. Meesapyodsuk D, Balsevich J, Reed DW, Covello PS (2007) Saponin biosynthesis in Saponaria vaccaria cDNAs encoding beta-amyrin synthase and a triterpene carboxylic acid glucosyl-transferase. Plant Physiol 143:959-969 (Pubitemid 46940955)
24. Nicot N, Hausman JF, Hoffmann L, Evers D (2005) Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 56:2907-2914 (Pubitemid 41507227)
25. Sui C, Wei JH, Chen SL, Chen HQ, Dong LM, Yang CM (2010) Construction of a full-length enriched cDNA library and analysis of 3111 ESTs from root of Bupleurum chinense DC. Bot Stud 51(1):7-16
26. Dong LM, Liu YJ, Wei JH (2008) Cloning of critical gene fragments in saikosaponin biosynthesis and associated sequence analysis. World Sci Technol Mod Tradit Chin Med 10(5):56-60, 15 (in Chinese)
27. Yang YF, Hou S, Cui GH, Chen SL, Wei JH, Huang LQ (2010) Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Salvia miltiorrhiza. Mol Biol Rep 37(1):507-513
28. Chen LR, Chen YJ, Lee CY, Lin TY (2007) MeJA induced transcriptional changes in adventitious roots of Bupleurum kaoi. Plant Sci 173:12-24
29. Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR (2005) Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139:5-17 (Pubitemid 43951587)
30. Liu NK, Xu XM (2006) Beta-tubulin is a more suitable internal control than beta-actin in western blot analysis of spinal cord tissues after traumatic injury. J Neurotrauma 23(12):1794-1801
31. Strube C, Buschbaum S, Wolken S, Schnieder T (2008) Evaluation of reference genes for quantitative real-time PCR to investigate protein disulfide isomerase transcription pattern in the bovine lungworm Dictyocaulus viviparous. Gene 425(1-2):36-43
32. Wang YC, Guo BH, Zhang F, Yao HY, Miao ZQ, Tang KX (2007) Molecular cloning and functional analysis of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from hazel (Corylus avellana L. Gasaway). J Biochem Mol Biol 40(6):861-869 (Pubitemid 350242269)
33. Liao P, Zhou W, Zhang L, Wang J, Yan XM, Zhang Y, Zhang R, Li L, Zhou GY, Kai GY (2009) Molecular cloning, characterization and expression analysis of a new gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Salvia miltiorrhiza. Acta Physiol Plant 31:565-572
34. Cao XY, Zong ZM, Ju XY, Sun Y, Dai CC, Liu Q, Jiang JH (2010) Molecular cloning, characterization and function analysis of the gene encoding HMG-CoA reductase from Euphorbia Pekinensis Rupr. Mol Biol Rep 37(3):1559-1567
35. Narváez JA, Canto Canché BB, Pérez PF, Madrid RR (2001) Differential expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) during flower and fruit development of Bixa orellana. J Plant Physiol 158(11):1471-1477 (Pubitemid 33146284)
36. Pan XC, Chen M, Liu Y, Wang Q, Zeng LJ, Li LQ, Liao ZH (2007) A new isopentenyl diphosphate isomerase gene from Camptotheca acuminata: cloning, characterization and functional expression in Escherichia coli. Mitochondrial DNA 19(2):98-105
37. Cunillera N, Boronat A, Ferrer A (1997) The Arabidopsis thali-ana FPS1 gene generates a novel mRNA that encodes a mito-chondrial farnesyl diphosphate synthase isoform. J Biol Chem 272:15381-15388 (Pubitemid 27255562)
38. Kim OT, Bang KH, Jung SJ, Kim YC, Hyun DY, Kim SH, Cha SW (2010) Molecular characterization of ginseng farnesyl diphosphate synthase gene and its up-regulation by methyl jasmonate. Biol Plant 54:47-53
39. Tani T, Katsuki T, Kubo M, Arichi S (1986) Histochemistry. IX: Distribution of saikosaponins in Bupleurum falcatum root. J Chromatogr 360:407-416
40. Kushiro T, Shibuya M, Ebizuka Y (1998) Cloning of oxido-squalene cyclase that catalyzes the formation of the most popular triterpene among higher plants. Eur J Biochem 256:238-244 (Pubitemid 28399118)
41. Haralampidis K, Bryan G, Qi X, Papadopoulou K, Bakht S, Melton R, Osbourn A (2001) A new class of oxidosqualene cyclases directs synthesis of antimicrobial phytoprotectants in monocots. Proc Natl Acad Sci USA 98(23):13431-13436 (Pubitemid 33051380)
42. Cammareri M, Consiglio MF, Pecchia P, Corea G, Lanzotti V, Ibeas JI, Tava A, Conicella C (2008) Molecular characterization of b-amyrin synthase from Aster sedifolius L. and triterpenoid saponin analysis. Plant Sci 175:255-261