Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Salvia miltiorrhiza

Molecular Biology Reports

Volumn 37, Issue 1, 2010, Pages 507-513

  Yang, Yanfang ^a   Hou, Shuang ^a,c   Cui, Guanghong ^b   Chen, Shilin ^a   Wei, Jianhe ^a   Huang, Luqi ^b  

^a INSTITUTE OF MEDICINAL PLANT DEVELOPMENT   (China)

^b INSTITUTE OF CHINESE MATERIA MEDICA   (China)

^c SOUTHWEST UNIVERSITY   (China)

Author keywords

DXR; Quantitative real time PCR; Reference gene; Salvia miltiorrhiza

Indexed keywords

1 DEOXY DEXTRO XYLULOSE 5 PHOSPHATE REDUCTOISOMERASE; ACTIN; ALPHA TUBULIN; BETA TUBULIN; ELONGATION FACTOR 1ALPHA; RNA 18S; UBIQUITIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARTICLE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PETAL; PISTIL; PLANT ROOT; PLANT STEM; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SALVIA MILTIORRHIZA; SEPAL; STAMEN;

GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; ORGAN SPECIFICITY; PLANT PROTEINS; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALVIA MILTIORRHIZA; SEQUENCE ANALYSIS, DNA; SOFTWARE; TRANSCRIPTION, GENETIC;

SALVIA MILTIORRHIZA;

EID: 77949263647     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9703-3     Document Type: Article

References (39)

1. SA Bustin 2002 Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays J Mol Endocrinol 25 169 193 10.1677/jme.0.0250169
2. DJ Mahoney K Carey MH Fu R Snow D Cameron-Smith G Parise MA Tarnopolsky 2004 Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise Physiol Genomics 18 226 231 10.1152/physiolgenomics. 00067.2004 1:CAS:528:DC%2BD2cXntF2jsbk%3D 15161965 (Pubitemid 39319503)
3. O Thellin W Zorzi B Lakaye B De Borman B Coumans G Hennen T Grisar A Igout E Heinen 1999 Housekeeping genes internal standards: use and limits J Biotechnol 75 291 295 10.1016/S0168-1656(99)00163-7 1:CAS:528:DyaK1MXmsFajt74%3D 10617337 (Pubitemid 29486627)
4. JA Warrington A Nair M Mahadevappa M Tsyganskaya 2000 Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes Physiol Genomics 2 143 147 1:CAS:528:DC%2BD3cXltFKns70%3D 11015593
5. SR Stürzenbaum P kille 2001 Control genes in quantitative molecular biological techniques: the variability of invariance Comp Biochem Physiol B 130 281 289 10.1016/S1096-4959(01)00440-7 11567890 (Pubitemid 32913186)
6. A Radonic S Thulke IM Mackay O Landt W Siegert A Nitsche 2004 Guideline to reference gene selection for quantitative real-time PCR Biochem Biophys Res Commun 313 856 862 10.1016/j.bbrc.2003.11.177 1:CAS:528:DC%2BD2cXosFak 14706621 (Pubitemid 38050071)
7. T Suzuki PJ Higgins DR Crawford 2000 Control selection for RNA quantitation Biotechniques 29 332 337 1:CAS:528:DC%2BD3cXls12jsr0%3D 10948434
8. PD Lee R Sladek CMT Greenwood HJ Hudson 2001 Control genes and variability: absence of ubiquitous reference transcripts in diverse mammalian expression studies Genome Res 12 292 297 10.1101/gr.217802
9. T Czechowski M Stitt T Altmann MK Udvardi WR Scheible 2005 Genomewide identification and testing of superior reference genes for transcript normalization in Arabidopsis Plant Physiol 139 5 17 10.1104/pp.105.063743 1:CAS:528:DC%2BD2MXhtVCgur7I 16166256 (Pubitemid 43951587)
10. E Spanakis 1993 Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls Nucleic Acids Res 21 3809 3819 10.1093/nar/21.16.3809 1:CAS:528:DyaK3sXmsVaktb8%3D 8367299
11. AM Brunner VB Busov SH Strauss 2004 Poplar genome sequence: functional genomics in an ecologically dominant plant species Trends Plant Sci 9 49 56 10.1016/j.tplants.2003.11.006 1:CAS:528:DC%2BD2cXjtFaksg%3D%3D 14729219 (Pubitemid 38157646)
12. SA Bustin 2002 Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems J Mol Endocrinol 29 23 39 10.1677/jme.0.0290023 1:CAS:528:DC%2BD38XmvFOhu7s%3D 12200227 (Pubitemid 34965744)
13. J Vandesompele K De Preter F Pattyn B Poppe N Van Roy A De Paepe F Speleman 2002 Accurate normalization of real time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biol 3 7 research0034.1 research0034.11 10.1186/gb-2002-3-7-research0034
14. TD Schmittgen BA Zakrajsek 2000 Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative J. Biochem. Biophys Method 46 69 81 10.1016/S0165-022X(00)00129-9 1:CAS:528: DC%2BD3cXot12rt78%3D (Pubitemid 30844902)
15. M Stöcher V Leb J Berg 2002 A convenient approach to the generation of multiple internal control DNA for a panel of real-time PCR assays J Virol Methods 108 1 8 10.1016/S0166-0934(02)00266-5
16. M Stöcher V Leb G Hölzl J Berg 2002 A simple approach to the generation of heterologous competitive internal controls for real-time PCR assays on the lightcycler J Clin Virol 25 S47 S53 10.1016/S1386-6532(02)00192-0 12467777
17. H Savli A Karadenizli F Kolayli S Gundes U Ozbek H Vahaboglu 2003 Expression stability of six housekeeping genes: a proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR J Med Microbiol 52 403 408 10.1099/jmm.0.05132-0 1:CAS:528: DC%2BD3sXltVGqtbo%3D 12721316 (Pubitemid 36589166)
18. BR Kim HY Nam SU Kim SI Kim YJ Chang 2003 Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice Biotechnol Lett 25 1869 1872 10.1023/A:1026298032009 1:CAS:528:DC%2BD3sXotlajsLc%3D 14677714 (Pubitemid 37436053)
19. AM Brunner IA Yakovlev SH Strauss 2004 Validating internal controls for quantitative plant gene expression studies BMC Plant Biol 4 14 10.1186/1471-2229-4-14 15317655 (Pubitemid 39364131)
20. N Nicot JF Hausman L Hoffman D Evers 2005 Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress J Exp Bot 56 421 2907 2914 10.1093/jxb/eri285 1:CAS:528:DC%2BD2MXhtFCjt7jJ 16188960 (Pubitemid 41507227)
21. M Libault S Thibivilliers DD Bilgin O Radwan M Benitez SJ Clough G Stacey 2008 Identification of four soybean reference genes for gene expression normalization Plant Genome 1 1 44 54 10.3835/plantgenome2008.02.0091 1:CAS:528:DC%2BD1cXht1Kgt77J
22. M Expósito-Rodríguez AA Borges A Borges-Pérez JA Pérez 2008 Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process BMC Plant Biol 8 131 10.1186/1471-2229-8-131 19102748
23. JY Han JY Fan Y Horie S Miura DH Cui H Ishii T Hibi H Tsuneki I Kimura 2008 Ameliorating effects of compounds derived from Salvia miltiorrhiza root extract on microcirculatory disturbance and target organ injury by ischemia and reperfusion Pharmacol Ther 117 280 295 10.1016/j.pharmthera.2007.09.008 1:CAS:528:DC%2BD1cXhtV2it78%3D 18048101
24. LY Zhou XX Zhu 2005 Progresses in studies of the pharmacokinetics of Danshen and its chemical components Chinese J Exptl Tradit Med Formul 11 3 66 69 1:CAS:528:DC%2BD28XhtVyrsL7N
25. SM Kim T Kuzuyama YJ Chang KS Song SU Kim 2006 Identification of class 2 1-deoxy-d-xylulose 5-phosphate synthase and 1-deoxy-d-xylulose 5-phosphate reductoisomerase genes from Gingo biloba and their transcription in embryo culture with respect to ginkgolide biosynthesis Planta Med 72 234 240 10.1055/s-2005-916180 1:CAS:528:DC%2BD28Xjt1Glt7k%3D 16534728 (Pubitemid 44037357)
26. SJ Wu M Shi JY Wu 2009 Cloning and characterization of the 1-deoxy-d-xylulose 5-phosphate reductoisomerase gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza (Chinese sage) hairy roots Biotechnol Appl Biochem 52 89 95 10.1042/BA20080004 1:CAS:528:DC%2BD1MXhsFSjtw%3D%3D 18302535
27. Yan XM, Zhang L, Wang J, Liao P, Zhang Y, Zhang R, Kai GY (2009) Molecular characterization and expression of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) gene from Salvia miltiorrhiza. Acta Physiol Plant. doi: 10.1007/s11738-009-0320-5
28. JC Leplé R Dauwe K Morreel V Storme C Lapierre B Pollet A Naumann KY Kang H Kim K Ruel A Lefèbvre JP Joseleau J Grima-Pettenati RD Rycke Sara Andersson-Gunnerås A Erban I Fehrle M Petit-Conil J Kopka A Polle E Messens B Sundberg SD Mansfield J Ralph G Pilate W Boerjan 2007 Down regulation of cinnamoyl-coenzyme a reductase in poplar: multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure Plant Cell 19 3669 3691 10.1105/tpc.107.054148 18024569
29. YF Yang J Wu K Zhu LQ Liu FD Chen DY Yu 2009 Identification and characterization of two chrysanthemum (Dendronthema × moriforlium) DREB genes, belonging to the AP2/EREBP family Mol Biol Rep 36 71 81 10.1007/s11033-007-9153-8 1:CAS:528:DC%2BD1cXhtl2mu7vN 17922220
30. M Rajinikanth SA Harding CJ Tsai 2007 The glycine decarboxylase complex multienzyme family in Populus J Exp Bot 58 7 1761 1770 10.1093/jxb/erm034 1:CAS:528:DC%2BD2sXmsFeis74%3D 17355947 (Pubitemid 47356014)
31. SH Chang J Ying JJ Zhang JY Su YJ Zeng YP Tong B Li ZS Li 2003 Expression of a Wheat S-like RNase (WRN1) cDNA during natural- and dark-induced senescence Acta Botanica Sinica 45 9 1071 1075 1:CAS:528:DC%2BD2cXhtFGgurrM
32. JS Coker E Davies 2003 Selection of candidate housekeeping controls in tomato plants using EST data Bio Techniques 35 740 748 1:CAS:528: DC%2BD3sXot1ymt74%3D
33. M Jain A Nijhawan AK Tyagi JP Khurana 2006 Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR Biochem Biophys Res Commun 345 646 651 10.1016/j.bbrc.2006.04.140 1:CAS:528:DC%2BD28XltVert70%3D 16690022 (Pubitemid 43776982)
34. B Jian B Liu Y Bi W Hou C Wu T Han 2008 Validation of internal control for gene expression study in soybean by quantitative real-time PCR BMC Mol Biol 9 59 10.1186/1471-2199-9-59 18573215 (Pubitemid 351974293)
35. L Carretero-Paulet I Ahumada N Cunillera M Rodríguez- Concepción A Ferrer A Boront N Campos 2002 Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-c-methyl-d-erythritol 4-phosphate pathway Plant Physiol 129 1581 1591 10.1104/pp.003798 1:CAS:528:DC%2BD38Xmtl2gsbY%3D 12177470 (Pubitemid 34923221)
36. YF Gong ZH Liao M Chen KJ Zuo L Guo QM Tan ZS Huang GY Kai XF Sun F Tan KX Tang 2005 Molecular cloning and characterization of a 1-deoxy-d-xylulose 5-phosphate reductoisomerase gene from Ginkgo biloba DNA Seq 16 2 111 120 1:CAS:528:DC%2BD2MXls1Gmu7g%3D 16147862
37. H Yao Y Gong K Zuo H Ling C Qiu F Zhang Y Wang Y Pi X Liu X Sun K Tang 2008 Molecular cloning, expression profiling and functional analysis of a DXR gene encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase from Camptotheca acuminata J plant physiol 165 2 203 213 10.1016/j.jplph.2006.12.001 1:CAS:528:DC%2BD1cXisFSmu7g%3D 17257708
38. MA Phillips P León A Boronat M Rodríguez-Concepción 2008 The plastidial MEP pathway: unified nomenclature and resources Trends Plant Sci 3 12 619 623 10.1016/j.tplants.2008.09.003
39. M Rodríguez-Concepción I Ahumada E Diez-Juez S Sauret-Güeto LM Lois F Gallego L Carretero-Paulet N Campos A Boronat 2001 1-deoxy-d-xylulose 5-phosphate reductoisomerase and plasmid isoprenoid biosynthesis during tomato fruit ripening Plant J 27 3 213 222 10.1046/j.1365-313x.2001.01089.x 11532167 (Pubitemid 32778812)