Site-directed mutagenesis of human cytosolic sulfotransferase (SULT) 2B1b to phospho-mimetic Ser348Asp results in an isoform with increased catalytic activity

Journal of Steroid Biochemistry and Molecular Biology

Volumn 127, Issue 3-5, 2011, Pages 315-323

  Salman, Emily D ^a   He, Dongning ^a   Runge Morris, Melissa ^b   Kocarek, Thomas A ^b   Falany, Charles N ^a  

^a University of Alabama at Birmingham   (United States)

^b WAYNE STATE UNIVERSITY   (United States)

Author keywords

Cholesterol; Dehydroepiandrosterone; Hydroxysteroid; Metabolism; Nuclear translocation; Phosphorylation; Sulfotransferase

Indexed keywords

ASPARTIC ACID; HISTIDINE; HOMODIMER; ISOENZYME; PRASTERONE; RECOMBINANT ENZYME; SERINE; SULFOTRANSFERASE; SULFOTRANSFERASE 2B1B; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CYTOSOL; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR MODEL; MUTATION; NUCLEAR LOCALIZATION SIGNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; SITE DIRECTED MUTAGENESIS; SULFATION; THERMOSTABILITY; WILD TYPE;

ASPARTIC ACID; BASE SEQUENCE; BIOCATALYSIS; CATALYSIS; CHROMATOGRAPHY, GEL; CYTOSOL; DNA PRIMERS; HUMANS; ISOENZYMES; MODELS, MOLECULAR; MOLECULAR MIMICRY; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; SERINE; SULFOTRANSFERASES;

EID: 81255157585     PISSN: 09600760     EISSN: 18791220     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2011.07.010     Document Type: Article

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