Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin

Protein Expression and Purification

Volumn 81, Issue 2, 2012, Pages 193-200

  Santos, Sandra P ^a   Bandeiras, Tiago M ^a,b   Pinto, Ana F ^a   Teixeira, Miguel ^a   Carrondo, Maria A ^a   Romão, Célia V ^a  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Hydrogen peroxide; Metalloprotein; Oxidative stress; Rrc; Rubrerythrin

Indexed keywords

BACTERIAL PROTEIN; BUFFER; DESULFOREDOXIN; HEMERYTHRIN; IRON SULFUR PROTEIN; RECOMBINANT PROTEIN; RUBREDOXIN; RUBRERYTHRINS;

ARTICLE; BACTERIAL GENE; CAMPYLOBACTER JEJUNI; CHEMISTRY; CRYSTALLIZATION; ESCHERICHIA COLI; FLUORESCENCE; FLUOROMETRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; OSMOLARITY; PH; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; STANDARD; TEMPERATURE; THERMODYNAMICS;

BACTERIAL PROTEINS; BUFFERS; CAMPYLOBACTER JEJUNI; CLONING, MOLECULAR; CRYSTALLIZATION; ESCHERICHIA COLI; FLUORESCENCE; FLUOROMETRY; GENES, BACTERIAL; HEMERYTHRIN; HYDROGEN-ION CONCENTRATION; IRON-SULFUR PROTEINS; OSMOLAR CONCENTRATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RUBREDOXINS; TEMPERATURE; THERMODYNAMICS;

CAMPYLOBACTER JEJUNI;

EID: 81155128023     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.10.001     Document Type: Article

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