Differential modulation of apoptotic gene expression by N-acetyl-l-cysteine in Leydig cells stimulated persistently with hCG in vivo

Molecular and Cellular Endocrinology

Volumn 348, Issue 1, 2012, Pages 155-164

  Aggarwal, Archana ^a,b   Misro, M M ^a   Maheshwari, Ankur ^a   Sehgal, Neeta ^b  

^a NATIONAL INSTITUTE OF HEALTH AND FAMILY WELFARE   (India)

^b UNIVERSITY OF DELHI   (India)

Author keywords

Apoptosis; Cell survival; Chronic hCG administration; Leydig cells; NAC co administration; Oxidative stress

Indexed keywords

ACETYLCYSTEINE; ANTIOXIDANT; CASPASE 3; CASPASE 8; CASPASE 9; CHORIONIC GONADOTROPIN; CYCLIC AMP; FAS ANTIGEN; FAS LIGAND; GLUTATHIONE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN BAX; PROTEIN BCL 2; PROTEIN KINASE B; STRESS ACTIVATED PROTEIN KINASE; TESTOSTERONE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; GENE EXPRESSION; IN VIVO STUDY; LEYDIG CELL; LIPID PEROXIDATION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; SPERMATOGENESIS; TESTOSTERONE BLOOD LEVEL; UPREGULATION;

ACETYLCYSTEINE; ANIMALS; APOPTOSIS; CASPASE 3; CATALASE; CHORIONIC GONADOTROPIN; CYCLIC AMP; FREE RADICAL SCAVENGERS; GLUTATHIONE; GLUTATHIONE TRANSFERASE; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LEYDIG CELLS; MALE; ORGAN SIZE; OXIDATIVE STRESS; POLY(ADP-RIBOSE) POLYMERASES; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCTIVE CONTROL AGENTS; SPERMATOGENESIS; SUPEROXIDE DISMUTASE; TESTIS; TESTOSTERONE; THIOBARBITURIC ACID REACTIVE SUBSTANCES; TUMOR SUPPRESSOR PROTEIN P53;

EID: 80755163191     PISSN: 03037207     EISSN: 18728057     Source Type: Journal    
DOI: 10.1016/j.mce.2011.08.002     Document Type: Article

References (51)

1. Aebi H. Catalse in vitro. Methods Enzymol. 1984, 105:121-126.
2. Aggarwal A., Misro M.M., Maheshwari A., Sehgal N., Nandan D. Adverse effects associated with persistent stimulation of Leydig cells with hCG in vitro. Mol. Reprod. Dev. 2009, 76:1076-1083.
3. Aggarwal A., Misro M.M., Maheshwari A., Sehgal N., Nandan D. N-Acetylcysteine counteracts oxidative stress and prevents hCG-induced apoptosis in rat Leydig cells through down regulation of caspase-8 and JNK. Mol. Reprod. Dev. 2010, 77:900-909.
4. Aktas B.K., Bulut S., Bulut S., Baykam M.M., Ozden C., Senes M., Yucel D., Memis A. The effects of N-acetylcysteine on testicular damage in experimental testicular ischemia/reperfusion injury. Pediatr. Surg. Int. 2010, 26(3):293-298.
5. Amsterdam A., Hanoch T., Dentes A., Tajima K., Strauss J.F., Seger R. Mechanisms of gonadotropin desensitization. Mol. Cell. Endocrinol. 2002, 187:69-74.
6. Anakwe O.O., Moger W.H. Catecholamine stimulation of androgen production by rat Leydig cells. Interactions with luteinizing hormone and luteinizing hormone-releasing hormone. Biol. Reprod. 1986, 35:806-814.
7. Aycan Z., Ust€unsalih-Inan Y., Cetinkaya E., Vidinlisan S., Ornek A. Evaluation of low-dose hCG treatment for cryptorchidism. Turkish J. Pediatr. 2006, 48:228-231.
8. Badawy A., El Nashar A.B., El Totongy M. Clomiphene citrate plus N-acetyl cysteine versus clomiphene citrate for augmenting ovulation in the managment of unexplined infertility: a randomized double-blind controlled trial. Fertil. Steril. 2006, 86:647-650.
9. Bergh A., Damber J.E., Hjertkvist M. Human chorionic gonadotrophin-induced testicular inflammation may be related to increased sensitivity to interleukin-1. Int. J. Androl. 1996, 19:229-236.
10. Bradford M.M. A rapid and sensitive method for the qunatitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 7:248-254.
11. Catt K.J., Harwood J.P., Claton R.N., Davies T.F., Chan V., Katikineni M., Nozu K., Dufau M.L. Regulation of peptide hormone receptors and gonadal steroidogenesis. Recent Prog. Horm. Res. 1980, 36:557-622.
12. Chilvers C., Dudley N.E., Gough M.H., Jackson M.B., Pike M.C. Undescended testis: the effect of treatment on subsequent risk of sub fertility and malignancy. J. Pediatr. Surg. 1986, 21:691-696.
13. Ciftci H., Verit A., Savas M., Yeni E., Erel O. Effects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. Urology 2009, 74:73-76.
14. Das K., Samanta L., Chainy G.B.N. A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals. Indian J. Biochem. Biophys. 2000, 237:201-204.
15. De Flora S., Grassi C., Carati L. Attenuation of influenza like symptomatology and improvement of cell mediated immunity with long term N-acetylcysteine treatment. Eur. Respir. J. 1997, 10:1535-1541.
16. De Flora S., Izzotti A., D'Agostini F., Balansky R.M. Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 2004, 22:999-1013.
17. Duarte F., Blaya R., Teloken P.E., Becker D., Fernandes M., Rhoden E.L. The effects of N-acetylcysteine on spermatogenesis and degree of testicular germ cell apoptosis in an experimental model of varicocele in rats. Int. Urol. Nephrol. 2010, 42(3):603-608.
18. Dunkel L., Taskinen S., Hovatta O., Tilly J.L., Wikstrom S. Germ cell apoptosis after treatment of cryptorchidism with human chorionic gonadotropin is associated with impaired reproductive function in the adult. J. Clin. Invest. 1997, 100:2341-2346.
19. Erkkila K., Hirvonen V., Wuokko E., Parvinen M., Dunkel L. N-Acetyl-l-cysteine inhibits apoptosis in human male germ cells in vitro. J. Clin. Endocrinol. Metab. 1998, 88:2523-2530.
20. Freeman D.A., Ascoli M. Desensitization of steroidogenesis in cultured Leydig tumor cells: role of cholesterol. PNAS USA. 1982, 79:7796-7800.
21. Fujisawa M., Kanzaki M., Tatsumi N., Okuda Y., Okada H., Arakawa S., Kamidono S. Inhibition of apoptosis in cultured immature rat Leydig cells by human chorionic gonadotropin associated with Bcl-2 mRNA expression. Endocr. Res. 2000, 26:59-70.
22. Gautam D.K., Misro M.M., Chaki S.P., Kaushik M., Sehgal N. HCG treatment increases H2O2 levels and induces germ cell apoptosis in the rat testis. Apoptosis 2007, 12(7):1173-1182.
23. Genestra M. Oxyl radicals, redox-sensitive signalling cascades and antioxidants. Cell. Signal. 2007, 19:1807-1819.
24. Gorman A., McGowan A., Cotter T.G. Role of peroxide and superoxide anion during tumor cell apoptosis. FEBS Lett. 1997, 404:27-33.
25. Habig W.H., Pabst M.J., Jakoby W.B. Glutathione-S-transferase. J. Biol. Chem. 1974, 249:7130-7139.
26. Hjertkvist M., Lackgren G., Ploen L., Bergh A. Does HCG treatment induce inflammation-like change in undescended testes in boys?. Pediatr. Surg. 1993, 28:254-258.
27. Kaleva M., Arsalo A., Louhimo I., Rapola J., Perheentupa J., Henriksen K., Toppari J. Treatment with human chorionic gonadotropin for cryptorchidism: clinical and histological effects. Int. J. Androl. 1996, 19:293-298.
28. Karaman I.M., Kaya C., Ozturk M., Pirincci N., Yimazgumrukcu G., Tuken M. The effects of human chorionic gonadotrophin on normal testicular tissue of rats: dose-dependence and reversibility. BJU Int. 2006, 97:1116-1118.
29. Kerr J.B., Sharpe R.M. Focal disruption of spermatogenesis in the testis of adult rats after a single administration of human chorionic gonadotropin. Cell Tissue Res. 1989, 257:163-169.
30. Kim D.K., Cho E.S., Um H.D. Caspase dependent and independent events in apoptosis induced by hydrogen peroxide. Exp. Cell Res. 2000, 257:82-88.
31. Kolon T.F., Miller O.F. Comparison of single versus multiple dose regimens for the human chorionic gonadotropin stimulatory test. J. Urol. 2001, 166:1451-1454.
32. 2 in vitro. FEBS J. 2009, 276:870-881.
33. 2 induced germ cell apoptosis through down-regulation of caspase-9 and JNK/c-Jun. Mol. Reprod. Dev. 2011, 78:69-79.
34. Manna P.R., Huhtaniemi I.T., Stocco D.M. Detection of hCG responsive expression of the steroidogenic acute regulatory protein in mouse Leydig cells. Biol. Proced. Online 2004, 6:83-93.
35. Mayer M., Noble M. N-Acetyl-l-cysteine is a pluripotent protector against cell death and enhancer of trophic factor-mediated cell survival in vitro. PNAS USA 1994, 91:7496-7500.
36. Mehta A., Sekhon C.P.S., Giri S., Orak J.K., Singh A.K. Attenuation of ischemia/reperfusion induced MAP kinase by N-acetyl cysteine, sodium nitroprusside and phosphoramidon. Mol. Cell. Biochem. 2002, 240:19-29.
37. Nicholson K.M., Anderson N.G. The protein kinase B/Akt signalling pathway in human malignancy. Cell. Signal. 2002, 14:381-395.
38. Ohkawa H., Ohishi N., Yagi K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979, 95:351-358.
39. Payabvash S., Salmasi A.H., Kiumehr S., Tavangar S.M., Nourbakhsh B., Faghihi S.H., Dehpour A.R. Salutary effects of N-acetylcysteine on apoptotic damage in a rat model of testicular torsion. Urol. Int. 2007, 79(3):248-254.
40. Peltola V., Huhtaniemi I., Metsa-Ketela T., Ahotupa M. Induction of lipid peroxidation during steroidogenesis in the rat testis. Endocrinology 1996, 137:105-112.
41. Pyorala S., Huttunen N.P., Uhari M. A review and meta-analysis of hormonal treatment of cryptorchidism. J. Clin. Endocrinol. Metab. 1995, 80:2795-2799.
42. Quinn P.G., Dombrausky L.J., Chen Y.-D.I., Payne A.H. Serum lipoproteins increase testosterone production in hCG desensitized Leydig cells. Endocrinology 1981, 109:1790-1792.
43. Reid A.J., Shawcross S.G., Hamilton A.E., Wiberg M., Terenghi G. N-Acetylcysteine alters apoptotic gene expression in axotomized primary sensory afferent sub-populations. Neurosci. Res. 2009, 65:148-155.
44. Roberfroid M., Calderon P.B. Free Radical and Oxidation Phenomena in Biological Systems 1995, Dekker, New York.
45. Roederer M., Ela S.W., Staal F.J., Herzenberg L.A. Effect of N-acetylcysteine: a new approach to anti-HIV therapy AIDS. Res. Hum. Retro-viruses 1992, 8:209-217.
46. Russell L.D., Clermont Y. Degeneration of germ cell in normal, hypophysectomized and hormone treated hypophysectomized rats. Anal. Rec. 1977, 187:347-366.
47. Stey C., Steurer J., Bachmann S., Medici T.C., Tramer M.R. The effects of oral N-acetylcysteine in chronic bronchitis: a quantitative systematic review. Eur. Respir. J. 2000, 16:253-262.
48. Tanel A., Averill-Bates D.A. Inhibition of acrolein-induced apoptosis by the antioxidant N-acetylcysteines. J. Pharmacol. Exp. Ther. 2007, 321:73-83.
49. Tang P.Z., Morris C.H., Dufau M.L. Regulation of 3b-hydroxysteroid dehydrogenase in gonadotropin-induced steroidogenic desensitization of Leydig cells. Endocrinology 1998, 139:4496-4505.
50. Wada T., Penninger J.M. Mitogen-activated protein kinases in apoptosis regulation. Oncogene 2004, 23:2838-2849.
51. Wu Y.J., Muldoon L.L., Neuwelt E.A. The Chemoprotective Agent N-acetylcysteine blocks cisplatin-induced apoptosis through caspase signaling pathway. J. Pharmacol. Exp. Ther. 2005, 312:424-431.