The amount of DNA damage needed to activate the radiation-induced G2 checkpoint varies between single cells

Radiotherapy and Oncology

Volumn 101, Issue 1, 2011, Pages 24-27

  Tkacz Stachowska, Kinga ^a   Lund Andersen, Christin ^a   Velissarou, Angeliki ^a   Myklebust, June H ^b   Stokke, Trond ^a   Syljuåsen, Randi G ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

H2AX; Checkpoint adaptation; DNA damage; G2 arrest; Ionizing radiation; Single cells

Indexed keywords

DNA; GAMMA HISTONE H2AX; HISTONE H2AX; HISTONE H3; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE G2 PHASE; CELL SELECTION; DIPLOIDY; DNA DAMAGE; FIBROBLAST; FLOW CYTOMETRY; GENOMIC INSTABILITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IRRADIATION; MITOSIS; OSTEOSARCOMA CELL; PRIORITY JOURNAL; RADIATION DOSE; SINGLE CELL ANALYSIS;

CELL SURVIVAL; DNA DAMAGE; DNA REPAIR; FIBROBLASTS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; G2 PHASE CELL CYCLE CHECKPOINTS; GAMMA RAYS; GENOMIC INSTABILITY; HISTONES; HUMANS; MITOSIS; OSTEOSARCOMA; RADIATION DOSAGE; REFERENCE VALUES; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 80655149132     PISSN: 01678140     EISSN: 18790887     Source Type: Journal    
DOI: 10.1016/j.radonc.2011.05.060     Document Type: Article

References (20)

1. G. Iliakis, Y. Wang, J. Guan, and H. Wang DNA damage checkpoint control in cells exposed to ionizing radiation Oncogene 22 2003 5834 5847 (Pubitemid 37176704)
2. H.P. Rodemann Molecular radiation biology: perspectives for radiation oncology Radiother Oncol 92 2009 293 298
3. C.S. Sorensen, M. Melixetian, D.K. Klein, and K. Helin NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling Cell cycle 9 2010 450 455
4. G.I. Terzoudi, K.N. Manola, G.E. Pantelias, and G. Iliakis Checkpoint abrogation in G2 compromises repair of chromosomal breaks in ataxia telangiectasia cells Cancer Res 65 2005 11292 11296 (Pubitemid 41821685)
5. D. Deckbar, J. Birraux, and A. Krempler Chromosome breakage after G2 checkpoint release J Cell Biol 176 2007 749 755 (Pubitemid 46425534)
6. A. Ishikawa, M. Yamauchi, K. Suzuki, and S. Yamashita Image-based quantitative determination of DNA damage signal reveals a threshold for G2 checkpoint activation in response to ionizing radiation Genome integrity 1 2010 10
7. R.G. Syljuasen, S. Jensen, J. Bartek, and J. Lukas Adaptation to the ionizing radiation-induced G2 checkpoint occurs in human cells and depends on checkpoint kinase 1 and Polo-like kinase 1 kinases Cancer Res 66 2006 10253 10257 (Pubitemid 44799740)
8. D.J. Galgoczy, and D.P. Toczyski Checkpoint adaptation precedes spontaneous and damage-induced genomic instability in yeast Mol Cell Biol 21 2001 1710 1718 (Pubitemid 32156391)
9. R.G. Syljuasen Checkpoint adaptation in human cells Oncogene 26 2007 5833 5839
10. M. Lobrich, and P.A. Jeggo The impact of a negligent G2/M checkpoint on genomic instability and cancer induction Nat Rev 7 2007 861 869 (Pubitemid 350006255)
11. K. Rothkamm, and M. Lobrich Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses Proc Natl Acad Sci U S A 100 2003 5057 5062 (Pubitemid 36542657)
12. P.O. Krutzik, and G.P. Nolan Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling Nat Methods 3 2006 361 368
13. U. Kasten-Pisula, A. Menegakis, and I. Brammer The extreme radiosensitivity of the squamous cell carcinoma SKX is due to a defect in double-strand break repair Radiother Oncol 90 2009 257 264
14. E.P. Rogakou, D.R. Pilch, A.H. Orr, V.S. Ivanova, and W.M. Bonner DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139 J Biol Chem 273 1998 5858 5868 (Pubitemid 28124064)
15. K. Koch, A. Wrona, E. Dikomey, and K. Borgmann Impact of homologous recombination on individual cellular radiosensitivity Radiother Oncol 90 2009 265 272
16. R.E. Meyn, A. Munshi, J.V. Haymach, L. Milas, and K.K. Ang Receptor signaling as a regulatory mechanism of DNA repair Radiother Oncol 92 2009 316 322
17. M. Suzuki, K. Suzuki, S. Kodama, and M. Watanabe Phosphorylated histone H2AX foci persist on rejoined mitotic chromosomes in normal human diploid cells exposed to ionizing radiation Radiat Res 165 2006 269 276
18. G. Iliakis Backup pathways of NHEJ in cells of higher eukaryotes: cell cycle dependence Radiother Oncol 92 2009 310 315
19. S. Giunta, R. Belotserkovskaya, and S.P. Jackson DNA damage signaling in response to double-strand breaks during mitosis J Cell Biol 190 2010 197 207
20. M.A. van Vugt, and R.H. Medema Getting in and out of mitosis with Polo-like kinase-1 Oncogene 24 2005 2844 2859 (Pubitemid 40638094)