Fabrication of electrochemical-DNA biosensors for the reagentless detection of nucleic acids, proteins and small molecules

Journal of Visualized Experiments

Volumn , Issue 52, 2011, Pages

  Rowe, Aaron A ^a   White, Ryan J ^a   Bonham, Andrew J ^a   Plaxco, Kevin W ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355135273     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2922     Document Type: Article

References (18)

1. Xiao, Y., Lai, R.Y. & Plaxco, K.W. Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing. Nat. Protocols 2, 2875-2880 (2007).
2. Ricci, F., Lai, R.Y., Heeger, A.J., Plaxco, K.W. & Sumner, J.J. Effect of Molecular Crowding on the Response of an Electrochemical DNA Sensor. Langmuir 23, 6827-6834 (2007).
3. White, R.J., Phares, N., Lubin, A.A., Xiao, Y. & Plaxco, K.W. Optimization of Electrochemical Aptamer-Based Sensors via Optimization of Probe Packing Density and Surface Chemistry. Langmuir 24, 10513-10518 (2008).
4. Lubin, A.A., Vander Stoep Hunt, B., White, R.J. & Plaxco, K.W. Effects of Probe Length, Probe Geometry, and Redox-Tag Placement on the Performance of the Electrochemical E-DNA Sensor. Analytical Chemistry 81, 2150-2158 (2009).
5. Lubin, A.A. & Plaxco, K.W. Folding-Based Electrochemical Biosensors: The Case for Responsive Nucleic Acid Architectures. Accounts of Chemical Research 43, 496-505 (2010).
6. Lubin, A.A., Lai, R.Y., Baker, B.R., Heeger, A.J. & Plaxco, K.W. Sequence-Specific, Electronic Detection of Oligonucleotides in Blood, Soil, and Foodstuffs with the Reagentless, Reusable E-DNA Sensor. Analytical Chemistry 78, 5671-5677 (2006).
7. Cash, K.J., Ricci, F. & Plaxco, K.W. An Electrochemical Sensor for the Detection of Protein?Small Molecule Interactions Directly in Serum and Other Complex Matrices. Journal of the American Chemical Society 131, 6955-6957 (2009).
8. Baker, B.R. et al. An Electronic, Aptamer-Based Small-Molecule Sensor for the Rapid, Label-Free Detection of Cocaine in Adulterated Samples and Biological Fluids. Journal of the American Chemical Society 128, 3138-3139 (2006).
9. Ferapontova, E.E., Olsen, E.M. & Gothelf, K.V. An RNA Aptamer-Based Electrochemical Biosensor for Detection of Theophylline in Serum. Journal of the American Chemical Society 130, 4256-4258 (2008).
10. Stojanovic, M.N. & Landry, D.W. Aptamer-Based Colorimetric Probe for Cocaine. Journal of the American Chemical Society 124, 9678-9679 (2002).
11. Stojanovic, M.N., Prada, P. & Landry, D.W. Aptamer-Based Folding Fluorescent Sensor for Cocaine. Journal of the American Chemical Society 123, 4928-4931 (2001).
12. Cash, K.J., Heeger, A.J., Plaxco, K.W. & Xiao, Y. Optimization of a Reusable, DNA Pseudoknot-Based Electrochemical Sensor for Sequence-Specific DNA Detection in Blood Serum. Analytical Chemistry 81, 656-661 (2009).
13. Xiao, Y. et al. An Electrochemical Sensor for Single Nucleotide Polymorphism Detection in Serum Based on a Triple-Stem DNA Probe. Journal of the American Chemical Society 131, 15311-15316 (2009).
14. Zuo, X., Xiao, Y. & Plaxco, K.W. High Specificity, Electrochemical Sandwich Assays Based on Single Aptamer Sequences and Suitable for the Direct Detection of Small-Molecule Targets in Blood and Other Complex Matrices. Journal of the American Chemical Society 131, 6944-6945 (2009).
15. Xia, F. et al. An Electrochemical Supersandwich Assay for Sensitive and Selective DNA Detection in Complex Matrices. Journal of the American Chemical Society 132, 14346-14348 (2010).
16. Patterson, A. et al. Using Triplex-Forming Oligonucleotide Probes for the Reagentless, Electrochemical Detection of Double-Stranded DNA. Analytical Chemistry 82, 9109-9115 (2010).
17. Ferguson, B.S. et al. Integrated Microfluidic Electrochemical DNA Sensor. Analytical Chemistry 81, 6503-6508 (2009).
18. Swensen, J.S. et al. Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor. Journal of the American Chemical Society 131, 4262-4266 (2009).