Hyperinsulinemic-euglycemic clamp in the conscious rat

Journal of Visualized Experiments

Volumn , Issue 48, 2010, Pages

  Hughey, Curtis C ^a   Hittel, Dustin S ^a   Johnsen, Virginia L ^a   Shearer, Jane ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Diabetes; Insulin Sensitivity; Issue 48; Medicine; Metabolism; Methodology

Indexed keywords

EID: 80355125430     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2432     Document Type: Article

References (11)

1. Halseth, A. E., Bracy, D. P. & Wasserman, D. H. Limitations to basal and insulin-stimulated skeletal muscle glucose uptake in the high-fat-fed rat. Am J Physiol Endocrinol Metab 279, E1064-1071 (2000).
2. Halseth, A. E., Bracy, D. P. & Wasserman, D. H. Limitations to muscle glucose uptake due to high fat feeding. Am. J. Physiol. 279, E1064-E1071 (2000).
3. Muniyappa, R., Lee, S., Chen, H. & Quon, M. J. Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage. Am J Physiol Endocrinol Metab 294, E15-26 (2008).
4. Cho, H. et al. Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science 292, 1728-1731 (2001).
5. Dubois, M. J. et al. The SHP-1 protein tyrosine phosphatase negatively modulates glucose homeostasis. Nat Med 12, 549-556 (2006).
6. Pacini, G., Thomaseth, K. & Ahren, B. Contribution to glucose tolerance of insulin-independent vs. insulin-dependent mechanisms in mice. Am J Physiol Endocrinol Metab 281, E693-703 (2001).
7. Herbach, N. et al. Dominant-negative effects of a novel mutated Ins2 allele causes early-onset diabetes and severe beta-cell loss in Munich Ins2C95S mutant mice. Diabetes 56, 1268-1276 (2007).
8. Maeda, N. et al. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med 8, 731-737 (2002).
9. Potenza, M. A., Marasciulo, F. L., Tarquinio, M., Quon, M. J. & Montagnani, M. Treatment of spontaneously hypertensive rats with rosiglitazone and/or enalapril restores balance between vasodilator and vasoconstrictor actions of insulin with simultaneous improvement in hypertension and insulin resistance. Diabetes 55, 3594-3603 (2006).
10. Ayala, J. E., Bracy, D. P., McGuinness, O. P. & Wasserman, D. H. Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse. Diabetes 55, 390-397 (2006).
11. Wasserman, D. H., Ayala, J. E. & McGuinness, O. P. Lost in translation. Diabetes 58, 1947-1950 (2009).