Quantitative measurement of GLUT4 translocation to the plasma membrane by flow cytometry

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Koshy, Shyny ^a   Alizadeh, Parema ^a   Timchenko, Lubov T ^a   Beeton, Christine ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355124190     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2429     Document Type: Article

References (7)

1. Zhao, F.Q. & Keating, A.F. Functional properties and genomics of glucose transporters. Curr. Genomics 8, 113-128 (2007).
2. Karnieli, E. & Armoni, M. Transcriptional regulation of the insulin-responsive glucose transporter GLUT4 gene: from physiology to pathology. Am. J. Endocrinol. Metab. 295, E38-45 (2008).
3. Graham, T.E. & Kahn, B.B. Tissue-specific alterations of glucose transport and molecular mechanisms of intertissue communication in obesity and type 2 diabetes. Horm. Metab. Res. 39, 717-721 (2007).
4. Betts, M. R., Brenchley, J. M., Price, D. A., De Rosa, S. C., Douek, D. C., Roederer, M. & Koup, R. A. Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J. Immunol. Methods 281, 65-78 (2003).
5. Alter, G., Malenfant, J.M. & Altfeld, M. CD107a as a functional marker for the identification of natural killer cell activity. J. Immunol. Methods 294, 15-22 (2004).
6. Vermes, I., Haanen, C., Steffens-Nakken, H. & Reutellingsperger, C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V. J. Immunol. Methods 184, 39-51 (1995).
7. Lugli, E., Roederer, M. & Cossarizza, A. Data analysis in flow cytometry: the future just started. Cytometry A. 77A, 705-713 (2010).