Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc

Biomaterials

Volumn 33, Issue 2, 2012, Pages 412-417

  Tavernier, Geertrui ^a   Wolfrum, Katharina ^b   Demeester, Joseph ^a   De Smedt, Stefaan C ^a   Adjaye, James ^b   Rejman, Joanna ^a  

^a GHENT UNIVERSITY   (Belgium)

^b MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

Liposome; MRNA; Non viral; Oct4; Reprogramming

Indexed keywords

ALKALINE PHOSPHATASE; CELL CLUSTERS; DIFFERENTIATED CELLS; GENOMIC INTEGRATION; INSERTIONAL MUTAGENESIS; MOUSE EMBRYONIC FIBROBLASTS; MRNA; NONVIRAL; NUCLEAR LOCALIZATION; OCT4; PLURIPOTENCY; PLURIPOTENT; PLURIPOTENT STEM CELLS; REPROGRAMMING; TRANSFECTION METHODS; TRANSFECTION PROTOCOL; UP-REGULATION;

CELL CULTURE; ENCODING (SYMBOLS); FIBROBLASTS; GENES; NUCLEIC ACIDS; PHOSPHATASES; STEM CELLS; TRANSCRIPTION; TRANSCRIPTION FACTORS;

MAMMALS;

ALKALINE PHOSPHATASE; KRUPPEL LIKE FACTOR 4; LIPID; MESSENGER RNA; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; STAGE SPECIFIC EMBRYO ANTIGEN 1; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ARTICLE; CELL ACTIVATION; EMBRYO CELL; FIBROBLAST; GENETIC TRANSFECTION; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; UPREGULATION;

ALKALINE PHOSPHATASE; ANIMALS; ANTIGENS, CD15; CELL DIFFERENTIATION; CELLS, CULTURED; FIBROBLASTS; GENE EXPRESSION REGULATION; INDUCED PLURIPOTENT STEM CELLS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MICE; NUCLEAR REPROGRAMMING; OCTAMER TRANSCRIPTION FACTOR-3; PROTO-ONCOGENE PROTEINS C-MYC; RNA, MESSENGER; SOXB1 TRANSCRIPTION FACTORS; TRANSFECTION; UP-REGULATION;

EID: 80055113187     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.09.062     Document Type: Article

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