Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51

Infection and Immunity

Volumn 68, Issue 6, 2000, Pages 3286-3289

  Vemulapalli, Ramesh ^a   He, Yongqun ^a   Cravero, Silvio ^a   Sriranganathan, Nammalwar ^a   Boyle, Stephen M ^a   Schurig, Gerhardt G ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN EXPRESSION; ARTICLE; BACTERIAL VIRULENCE; BRUCELLA ABORTUS; BRUCELLOSIS; DAIRY INDUSTRY; IMMUNOSTIMULATION; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ANTIGENS, BACTERIAL; BRUCELLA ABORTUS; BRUCELLA VACCINE; BRUCELLOSIS; BRUCELLOSIS, BOVINE; CATTLE; INTERFERON TYPE II; MICE; MICE, INBRED BALB C; RECOMBINANT PROTEINS; SPLEEN; SUPEROXIDE DISMUTASE; VACCINATION; VACCINES, ATTENUATED;

EID: 0034115440     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.6.3286-3289.2000     Document Type: Article

References (32)

1. Acha, P., and B. Szyfres. 1980. Zoonoses and communicable diseases common to man and animals, p. 28-45. Pan American Health Organization, Washington, D.C.
2. Araya, L. N., and A. J. Winter. 1990. Comparative protection of mice against virulent and attenuated strains of Brucella abortus by passive transfer of immune T cells or serum. Infect. Immun. 58:254-256.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Bricker, B. J., L. B. Tabatabai, B. A. Judge, B. L. Deyoe, and J. E. Mayfield. 1990. Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase. Infect. Immun. 58:2935-2939.
5. Corbel, M. J. 1997. Brucellosis: an overview. Emerg. Infect. Dis. 3:213-221.
6. Jimenez de Bagues, M. P., P. H. Elzer, S. M. Jones, J. M. Blasco, F. M. Enright, G. G. Schurig, and A. J. Winter. 1994. Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis. Infect. Immun. 62: 4990-4996.
7. Kerksiek, K. M., and E. G. Pamer. 1999. T cell responses to bacterial infection. Curr. Opin. Immunol. 11:400-405.
8. Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Roop II, and K. M. Peterson. 1994. pBBRIMCS: a broad-host-range cloning vector. BioTechniques 16:800-802.
9. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
10. Latimer, E., J. Simmers, N. Sriranganathan, R. M. Roop, G. G. Schurig, and S. M. Boyle. 1992. Brucella abortus deficient in copper/zinc superoxide dismutase is virulent in BALB/c mice. Microb. Pathog. 12:105-113.
11. ® system for mixed models. SAS Institute Inc., Cary, N.C.
12. McQuiston, J. R., G. G. Schurig, N. Sriranganathan, and S. M. Boyle. 1995. Transformation of Brucella species with suicide and broad host-range plasmids. Methods Mol. Biol. 47:143-148.
13. Oñate, A. A., R. Vemulapalli, E. Andrews, G. G. Schurig, S. M. Boyle, and H. Folch. 1999. Vaccination with live Escherichia coli expressing Brucella abortus Cu/Zn superoxide dismutase protects mice against virulent B. abortus. Infect. Immun. 67:986-988.
14. Papavinasasundaram, K. G., M. J. Colston, and E. O. Davis. 1998. Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein in Mycobacterium tuberculosis recA does not affect RecA function. Mol. Microbiol. 30:525-534.
15. Payton, M., R. Auty, R. Delgoda, M. Everett, and E. Sim. 1999. Cloning and characterization of arylamine N-acetyltransferase genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: increased expression results in isoniazid resistance. J. Bacteriol. 181:1343-1347.
16. Rafie-Kolpin, M., R. C. Essenberg, and J. H. Wyckoff. 1996. Identification and comparison of macrophage-induced proteins and proteins induced under various stress conditions in Brucella abortus. Infect. Immun. 64:5274-5283.
17. Rastogi, V., M. Labes, T. Finan, and R. Watson. 1992. Overexpression of the dctA gene in Rhizobium meliloti: effect on transport of C4 dicarboxylates and symbiotic nitrogen fixation. Can. J. Microbiol. 38:555-562.
18. Roop, R. M., II, M. L. Price, B. E. Dunn, S. M. Boyle, N. Sriranganathan, and G. G. Schurig. 1992. Molecular cloning and nucleotide sequence analysis of the gene encoding the immunoreactive Brucella abortus Hsp60 protein, BA60K. Microb. Pathog. 12:47-62.
19. Sanchez, J., and J. Holmgren. 1989. Recombinant system for overexpression of cholera toxin B subunit in Vibrio cholerae as a basis for vaccine development. Proc. Natl. Acad. Sci. USA 86:481-485.
20. Schurig, G. G., R. M. Roop, T. Bagchi, S. Boyle, D. Buhrman, and N. Sriranganathan. 1991. Biological properties of RB51: a stable rough strain of Brucella abortus. Vet. Microbiol. 28:171-188.
21. Silva, A., R. Fando, and J. A. Benitez. 1998. Overexpression of a mutant B subunit in toxigenic Vibrio cholerae diminishes production of active cholera toxin in vivo. Curr. Microbiol. 37:231-235.
22. Sriranganathan, N., S. M. Boyle, G. G. Schurig, and H. Misra. 1991. Superoxide dismutases of virulent and avirulent strains of Brucella abortus. Vet. Microbiol. 26:359-366.
23. Stevens, M. G., L. B. Tabatabai, S. C. Olsen, and N. F. Cheville. 1994. Immune responses to superoxide dismutase and synthetic peptides of superoxide dismutase in cattle vaccinated with Brucella abortus strain 19 or RB51. Vet. Microbiol. 41:383-389.
24. Stevens, T. L., A. Bossie, V. M. Sanders, R. Fernandez-Botran, R. L. Coffman, T. R. Mosmann, and E. S. Vitetta. 1988. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature 334:255-258.
25. Tabatabai, L. B., and G. W. Pugh Jr. 1994. Modulation of immune responses in Balb/c mice vaccinated with Brucella abortus Cu-Zn superoxide dismutase synthetic peptide vaccine. Vaccine 12:919-924.
26. Tatum, F. M., P. G. Detilleux, J. M. Sacks, and S. M. Halling. 1992. Construction of Cu-Zn superoxide dismutase deletion mutants of Brucella abortus: analysis of survival in vitro in epithelial and phagocytic cells and in vivo in mice. Infect. Immun. 60:2863-2869.
27. Vemulapalli, R., S. Cravero, C. L. Calvert, T. E. Toth, S. M. Boyle, N. Sriranganathan, and G. G. Schurig. 2000. Characterization of specific immune responses of mice inoculated with recombinant vaccinia virus expressing an 18-kDa outer membrane protein of Brucella abortus. Clin. Diagn. Lab. Immunol. 7:114-118.
28. Vemulapalli, R., A. J. Duncan, S. M. Boyle, N. Sriranganathan, T. E. Toth, and G. G. Schurig. 1998. Cloning and sequencing of yajC and secD homologs of Brucella abortus and demonstration of immune responses to YajC in mice vaccinated with B. abortus RB51. Infect. Immun. 66:5684-5691.
29. Vemulapalli, R., Y. He, S. M. Boyle, N. Sriranganathan, and G. G. Schurig. 2000. Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses. Infect. Immun. 68:3290-3296.
30. Winter, A. J., G. G. Schurig, S. M. Boyle, N. Sriranganathan, J. S. Bevins, F. M. Enright, P. H. Elzer, and J. D. Kope. 1996. Protection of BALB/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suis biovar 4. Am. J. Vet. Res. 57:677-683.
31. Wright, R., C. Stephens, and L. Shapiro. 1997 The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus. J. Bacteriol. 179:5869-5877.
32. Zhan, Y., and C. Cheers. 1993. Endogenous gamma interferon mediates resistance to Brucella abortus infection. Infect. Immun. 61:4899-4901.