T-wave ion mobility-mass spectrometry: Basic experimental procedures for protein complex analysis

Journal of Visualized Experiments

Volumn , Issue 41, 2010, Pages

  Michaelevski, Izhak ^a   Kirshenbaum, Noam ^a   Sharon, Michal ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055109876     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1985     Document Type: Article

References (16)

1. Ruotolo, B. T. et al., Ion mobility-mass spectrometry analysis of large proteincomplexes. Nat Protoc 3 (7), 1139-1152 (2008).
2. Scarff, C. A., Thalassinos, K., Hilton, G. R., and Scrivens, J. H., Travelling wave ion mobility mass spectrometry studies of protein structure: biological significance and comparison with X-ray crystallography and nuclear magnetic resonance spectroscopy measurements. Rapid Commun Mass Spectrom 22 (20), 3297-3304 (2008).
3. Smith, D. P. et al., Deciphering drift time measurements from travelling wave ion mobility spectrometry-mass spectrometry studies. Eur J Mass Spectrom (Chichester, Eng) 15 (2), 113-130 (2009).
4. Leary, J. A. et al., Methodology for measuring conformation of solvent-disrupted protein subunits using T-WAVE ion mobility MS: an investigation into eukaryotic initiation factors. J Am Soc Mass Spectrom 20 (9), 1699-1706 (2009).
5. Lorenzen, K. et al., Determination of stoichiometry and conformational changes in the first step of the P22 tail assembly. J Mol Biol 379 (2), 385-396 (2008).
6. Pukala, T. L. et al., Subunit architecture of multiprotein assemblies determined using restraints from gas-phase measurements. Structure 17 (9), 1235-1243 (2009).
7. van Duijn, E. et al., Chaperonin complexes monitored by ion mobility mass spectrometry. J Am Chem Soc 131 (4), 1452-1459 (2009).
8. Ruotolo, B. T. et al., Evidence for macromolecular protein rings in the absence of bulk water. Science 310 (5754), 1658-1661 (2005).
9. Ruotolo, B. T. and Robinson, C. V., Aspects of native proteins are retained in vacuum. Curr Opin Chem Biol 10 (5), 402-408 (2006).
10. Giles, K. et al., Applications of a travelling wave-based radio-frequency-only stacked ring ion guide. Rapid Commun Mass Spectrom 18 (20), 2401-2414 (2004).
11. McKay, A. R., Ruotolo, B. T., Ilag, L. L., and Robinson, C. V., Mass measurements of increased accuracy resolve heterogeneous populations of intact ribosomes. J Am Chem Soc 128 (35), 11433-11442 (2006).
12. Ruotolo, B. T. et al., Ion mobility-mass spectrometry reveals long-lived, unfolded intermediates in the dissociation of protein complexes. Angew Chem Int Ed Engl 46 (42), 8001-8004 (2007).
13. Morton, V. L., Stockley, P. G., Stonehouse, N. J., and Ashcroft, A. E., Insights into virus capsid assembly from non-covalent mass spectrometry. Mass Spectrom Rev 27 (6), 575-595 (2008).
14. Valentine, S. J., Counterman, A. E., and Clemmer, D. E., A database of 660 peptide ion cross sections: use of intrinsic size parameters for bona fide predictions of cross sections. J Am Soc Mass Spectrom 10 (11), 1188-1211 (1999).
15. Mesleh, M. F. et al., Structural information from ion mobility measurements: effects of the long-range potential. J Phys Chem 100, 16082-16086 (1996).
16. Shvartsburg, A. A. and Jarrold, M. F., An exact hard-spheres scattering model for the mobilities of polyatomic ions. Chem Phys Lett 261, 86-91 (1996).