Developing an RNase-free bioprocess to produce pharmaceutical-grade plasmid DNA using selective precipitation and membrane chromatography

Separation and Purification Technology

Volumn 83, Issue 1, 2011, Pages 121-129

  Zhong, Luyang ^a   Srirangan, Kajan ^a   Scharer, Jeno ^a   Moo Young, Murray ^a   Fenner, Drew ^b   Crossley, Lisa ^b   Howie Honeyman, C ^b   Suen, Shing Yi ^c   Perry Chou, C ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b Natrix Separations Inc   (Canada)

^c NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Anion exchange membrane chromatography; Calcium chloride; Isopropanol; Plasmid DNA; RNase free

Indexed keywords

ANION EXCHANGE MEMBRANE; BIOMEDICAL APPLICATIONS; BIOPROCESSES; CELLULAR RNA; GENOMIC DNA; HIGH EFFICIENCY; HIGH MOLECULAR WEIGHT; HIGH-CAPACITY; IMPURITY REMOVAL; ISO-PROPANOLS; ISOPROPANOL; LOW MOLECULAR WEIGHT; MEMBRANE CHROMATOGRAPHY; OPERATING COMPLEXITY; OPTIMAL INTEGRATION; PHARMACEUTICAL GRADE PLASMID DNA; PLASMID DNA; PRODUCT QUALITY; RNASE-FREE; SELECTIVE PRECIPITATION; SIMULTANEOUS ACHIEVEMENT; TRACE AMOUNTS;

BIOMOLECULES; CALCIUM; CALCIUM CHLORIDE; DESALINATION; DNA; DRIERS (MATERIALS); IMPURITIES; IONS; MEDICAL APPLICATIONS; MEMBRANES; PRECIPITATION (CHEMICAL); QUALITY CONTROL; REMOVAL; RNA;

CHROMATOGRAPHY;

ANIMALIA;

EID: 80055085488     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2011.09.024     Document Type: Article

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