Targeted cell fusion facilitates stable heterokaryon generation in vitro and in vivo

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Long, Michael A ^a   Rossi, Fabio M V ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA7 INTEGRIN; LENTIVIRUS VECTOR; MACROGOL; MYOD PROTEIN; MYOGENIN; NERVE CELL ADHESION MOLECULE; VIRUS HEMAGGLUTININ; MACROGOL DERIVATIVE; PRIMER DNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL FUSION; CONTROLLED STUDY; FIBROBLAST; HETEROKARYON; HISTONE ACETYLATION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MALE; MOUSE; MUSCLE REGENERATION; MYOTUBE; NONHUMAN; NUCLEAR REPROGRAMMING; SKELETAL MUSCLE; CELL LINE; CHROMATIN IMMUNOPRECIPITATION; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENT ANTIBODY TECHNIQUE; GENE VECTOR; GENETICS; LENTIVIRINAE; MEASLES VIRUS; METABOLISM; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION;

BASE SEQUENCE; CELL FUSION; CELL LINE; CHROMATIN IMMUNOPRECIPITATION; DNA PRIMERS; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC VECTORS; HEMAGGLUTININS, VIRAL; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; LENTIVIRUS; MEASLES VIRUS; POLYETHYLENE GLYCOLS; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 80055060431     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0026381     Document Type: Article

References (43)

1. Blau HM, Chiu CP, Webster C, (1983) Cytoplasmic activation of human nuclear genes in stable heterocaryons. Cell 32: 1171-1180.
2. Kohler G, Milstein C, (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497.
3. Gong J, Chen D, Kashiwaba M, Kufe D, (1997) Induction of antitumor activity by immunization with fusions of dendritic and carcinoma cells. Nat Med 3: 558-561.
4. Pontecorvo G, (1975) Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somatic Cell Genet 1: 397-400.
5. Zimmermann U, Vienken J, (1982) Electric field-induced cell-to-cell fusion. J Membr Biol 67: 165-182.
6. Stromberg A, Ryttsen F, Chiu DT, Davidson M, Eriksson PS, et al. (2000) Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion. Proc Natl Acad Sci U S A 97: 7-11.
7. Bakker Schut TC, Kraan YM, Barlag W, de Leij L, de Grooth BG, et al. (1993) Selective electrofusion of conjugated cells in flow. Biophys J 65: 568-572.
8. Skelley AM, Kirak O, Suh H, Jaenisch R, Voldman J, (2009) Microfluidic control of cell pairing and fusion. Nat Methods 6: 147-152.
9. Alvarez-Dolado M, Pardal R, Garcia-Verdugo JM, Fike JR, Lee HO, et al. (2003) Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Nature 425: 968-973.
10. Relvas JB, Aldridge H, Wells KE, Wells DJ, Watt DJ, (1997) Exogenous genes are expressed in mdx muscle fibres following the implantation of primary mouse skin cells. Basic and Applied Myology 7: 211-219.
11. Camargo FD, Green R, Capetanaki Y, Jackson KA, Goodell MA, (2003) Single hematopoietic stem cells generate skeletal muscle through myeloid intermediates. Nat Med 9: 1520-1527.
12. Warthin AS, (1931) Occurrence of numerous large giant cells in the tonsils and pharyngeal mucosa in the prodromal stage of measles- Report of four cases. Archives of Pathology 11: 864-874.
13. Okada Y, (1962) Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. I. Microscopic observation of giant polynuclear cell formation. Exp Cell Res 26: 98-107.
14. Dorig RE, Marcil A, Chopra A, Richardson CD, (1993) The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell 75: 295-305.
15. Tatsuo H, Ono N, Tanaka K, Yanagi Y, (2000) SLAM (CDw150) is a cellular receptor for measles virus. Nature 406: 893-897.
16. Navaratnarajah CK, Oezguen N, Rupp L, Kay L, Leonard VH, et al. (2011) The heads of the measles virus attachment protein move to transmit the fusion-triggering signal. Nat Struct Mol Biol 18: 128-134.
17. Hallak LK, Merchan JR, Storgard CM, Loftus JC, Russell SJ, (2005) Targeted measles virus vector displaying echistatin infects endothelial cells via alpha(v)beta3 and leads to tumor regression. Cancer Res 65: 5292-5300.
18. Schneider U, Bullough F, Vongpunsawad S, Russell SJ, Cattaneo R, (2000) Recombinant measles viruses efficiently entering cells through targeted receptors. J Virol 74: 9928-9936.
19. Peng KW, Donovan KA, Schneider U, Cattaneo R, Lust JA, et al. (2003) Oncolytic measles viruses displaying a single-chain antibody against CD38, a myeloma cell marker. Blood 101: 2557-2562.
20. Allen C, Paraskevakou G, Iankov I, Giannini C, Schroeder M, et al. (2008) Interleukin-13 displaying retargeted oncolytic measles virus strains have significant activity against gliomas with improved specificity. Mol Ther 16: 1556-1564.
21. Wild TF, Malvoisin E, Buckland R, (1991) Measles virus: both the haemagglutinin and fusion glycoproteins are required for fusion. J Gen Virol 72 (Pt 2): 439-442.
22. Blanco-Bose WE, Yao CC, Kramer RH, Blau HM, (2001) Purification of mouse primary myoblasts based on alpha 7 integrin expression. Exp Cell Res 265: 212-220.
23. Nakamura T, Peng KW, Vongpunsawad S, Harvey M, Mizuguchi H, et al. (2004) Antibody-targeted cell fusion. Nat Biotechnol 22: 331-336.
24. Palermo A, Doyonnas R, Bhutani N, Pomerantz J, Alkan O, et al. (2009) Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional. FASEB J 23: 1431-1440.
25. Chiu CP, Blau HM, (1985) 5-Azacytidine permits gene activation in a previously noninducible cell type. Cell 40: 417-424.
26. Terranova R, Pereira CF, Du Roure C, Merkenschlager M, Fisher AG, (2006) Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming. J Cell Sci 119: 2065-2072.
27. Zhang F, Pomerantz JH, Sen G, Palermo AT, Blau HM, (2007) Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons. Proc Natl Acad Sci U S A 104: 4395-4400.
28. Pomerantz JH, Mukherjee S, Palermo AT, Blau HM, (2009) Reprogramming to a muscle fate by fusion recapitulates differentiation. J Cell Sci 122: 1045-1053.
29. Yablonka-Reuveni Z, Rivera AJ, (1994) Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers. Dev Biol 164: 588-603.
30. Roh TY, Cuddapah S, Zhao K, (2005) Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping. Genes Dev 19: 542-552.
31. Bouchentouf M, Benabdallah BF, Mills P, Tremblay JP, (2006) Exercise improves the success of myoblast transplantation in mdx mice. Neuromuscul Disord 16: 518-529.
32. Skuk D, Roy B, Goulet M, Tremblay JP, (1999) Successful myoblast transplantation in primates depends on appropriate cell delivery and induction of regeneration in the host muscle. Exp Neurol 155: 22-30.
33. Plant DR, Colarossi FE, Lynch GS, (2006) Notexin causes greater myotoxic damage and slower functional repair in mouse skeletal muscles than bupivacaine. Muscle Nerve 34: 577-585.
34. Kouzarides T, (2007) Chromatin modifications and their function. Cell 128: 693-705.
35. Lipton BH, Schultz E, (1979) Developmental fate of skeletal muscle satellite cells. Science 205: 1292-1294.
36. Blau HM, Webster C, Pavlath GK, (1983) Defective myoblasts identified in Duchenne muscular dystrophy. Proc Natl Acad Sci U S A 80: 4856-4860.
37. Peault B, Rudnicki M, Torrente Y, Cossu G, Tremblay JP, et al. (2007) Stem and progenitor cells in skeletal muscle development, maintenance, and therapy. Mol Ther 15: 867-877.
38. Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, et al. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol 29: 341-345.
39. Iankov ID, Blechacz B, Liu C, Schmeckpeper JD, Tarara JE, et al. (2007) Infected cell carriers: a new strategy for systemic delivery of oncolytic measles viruses in cancer virotherapy. Mol Ther 15: 114-122.
40. Olivetti G, Cigola E, Maestri R, Corradi D, Lagrasta C, et al. (1996) Aging, cardiac hypertrophy and ischemic cardiomyopathy do not affect the proportion of mononucleated and multinucleated myocytes in the human heart. J Mol Cell Cardiol 28: 1463-1477.
41. Kudryavtsev BN, Kudryavtseva MV, Sakuta GA, Stein GI, (1993) Human hepatocyte polyploidization kinetics in the course of life cycle. Virchows Arch B Cell Pathol Incl Mol Pathol 64: 387-393.
42. Held PK, Al-Dhalimy M, Willenbring H, Akkari Y, Jiang S, et al. (2006) In vivo genetic selection of renal proximal tubules. Mol Ther 13: 49-58.
43. Wang X, Willenbring H, Akkari Y, Torimaru Y, Foster M, et al. (2003) Cell fusion is the principal source of bone-marrow-derived hepatocytes. Nature 422: 897-901.