Detection of single enzymatic events in rare or single cells using microfluidics

ACS Nano

Volumn 5, Issue 10, 2011, Pages 8305-8310

  Juul, Sissel ^a,b   Ho, Yi Ping ^b   Koch, Jørn ^c   Andersen, Felicie F ^a   Stougaard, Magnus ^c   Leong, Kam W ^b   Knudsen, Birgitta R ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b Duke University   (United States)

^c AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

enzyme activity; microfluidics; rolling circle amplification; single cell analysis; single molecule detection

Indexed keywords

ACTIVITY DETECTION; BASIC RESEARCH; HIGHLY SENSITIVE; HUMAN CELLS; INTEGRATED SYSTEMS; LOW CONCENTRATIONS; MICRO-FLUIDIC DEVICES; MULTIPLEXED DETECTION; PICOLITER DROPLETS; REACTION VOLUME; RELIABLE DETECTION; ROLLING CIRCLE AMPLIFICATION; SINGLE CELLS; SINGLE-CELL LEVEL; SINGLE-MOLECULE; SINGLE-MOLECULE DETECTION; SINGLECELL ANALYSIS; THERANOSTICS; WILD TYPES;

ENZYME ACTIVITY; MICROFLUIDICS; MOLECULES; REACTION KINETICS;

CELLS;

FLUORESCENT DYE; OLIGONUCLEOTIDE;

ARTICLE; CELL STRAIN HEK293; ENZYME ASSAY; GENETIC PROCEDURES; HUMAN; INSTRUMENTATION; KINETICS; METABOLISM; METHODOLOGY; MICROFLUIDIC ANALYSIS; SINGLE CELL ANALYSIS;

BIOSENSING TECHNIQUES; ENZYME ASSAYS; FLUORESCENT DYES; HEK293 CELLS; HUMANS; KINETICS; MICROFLUIDIC ANALYTICAL TECHNIQUES; OLIGONUCLEOTIDES; SINGLE-CELL ANALYSIS;

EID: 80054973509     PISSN: 19360851     EISSN: 1936086X     Source Type: Journal    
DOI: 10.1021/nn203012q     Document Type: Article

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