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Volumn 35, Issue 10, 2011, Pages 1055-1062

Characterization of bone-marrow-derived rat mesenchymal stem cells depending on donor age

Author keywords

Aging; Bone marrow; Cytokine; Interleukin 1a (IL 1a); Mesenchymal stem cell (MSC)

Indexed keywords

FIBROBLAST GROWTH FACTOR 2; HYDROGEN PEROXIDE; INTERLEUKIN 1ALPHA; TRANSFORMING GROWTH FACTOR BETA1; VASCULOTROPIN;

EID: 80054972499     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1042/CBI20100586     Document Type: Article
Times cited : (30)

References (42)
  • 1
    • 0035341209 scopus 로고    scopus 로고
    • TGF-beta-induced repression of CBFA1 by Smad3 decreases cbfa1 and osteocalcin expression and inhibits osteoblast differentiation
    • Alliston T, Choy L, Ducy P, Karsenty G, Derynck R. TGF-beta-induced repression of CBFA1 by Smad3 decreases cbfa1 and osteocalcin expression and inhibits osteoblast differentiation. EMBO J 2001;20:2254-72.
    • (2001) EMBO J , vol.20 , pp. 2254-2272
    • Alliston, T.1    Choy, L.2    Ducy, P.3    Karsenty, G.4    Derynck, R.5
  • 2
    • 63349096018 scopus 로고    scopus 로고
    • Preincubation with bFGF but not sodium ascorbate improves efficiency of autologous transplantation of muscle-derived cells into urethral wall
    • Burdzinska A, Bartoszuk U, Orzechowski A. Preincubation with bFGF but not sodium ascorbate improves efficiency of autologous transplantation of muscle-derived cells into urethral wall. Urology 2009;737:736-42.
    • (2009) Urology , vol.737 , pp. 736-742
    • Burdzinska, A.1    Bartoszuk, U.2    Orzechowski, A.3
  • 3
    • 3042662157 scopus 로고    scopus 로고
    • Effect on left ventricular function of intracoronary transplantation of autologous bone marrow mesenchymal stem cell in patients with acute myocardial infarction
    • Chen SL, Fang WW, Ye F, Liu YH, Qian J, Shan SJ et al. Effect on left ventricular function of intracoronary transplantation of autologous bone marrow mesenchymal stem cell in patients with acute myocardial infarction. Am J Cardiol 2004;94:92-5.
    • (2004) Am J Cardiol , vol.94 , pp. 92-95
    • Chen, S.L.1    Fang, W.W.2    Ye, F.3    Liu, Y.H.4    Qian, J.5    Shan, S.J.6
  • 4
    • 0034934262 scopus 로고    scopus 로고
    • Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells
    • Colter DC, Sekiya I, Prockop DJ. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA 2001;98:7841-5.
    • (2001) Proc Natl Acad Sci USA , vol.98 , pp. 7841-7845
    • Colter, D.C.1    Sekiya, I.2    Prockop, D.J.3
  • 5
    • 0032976690 scopus 로고    scopus 로고
    • Transplantability and therapeutic effects of bone marrowderived mesenchymal cells in children with osteogenesis imperfecta
    • Horwitz EM, Prockop DJ, Fitzpatrick LA, Koo WW, Gordon PL, Neel M et al. Transplantability and therapeutic effects of bone marrowderived mesenchymal cells in children with osteogenesis imperfecta. Nat Med 1999;5:309-13.
    • (1999) Nat Med , vol.5 , pp. 309-313
    • Horwitz, E.M.1    Prockop, D.J.2    Fitzpatrick, L.A.3    Koo, W.W.4    Gordon, P.L.5    Neel, M.6
  • 6
    • 0037173116 scopus 로고    scopus 로고
    • Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: implications for cell therapy of bone
    • Horwitz EM, Gordon PL, Koo WK, Marx JC, Neel MD, McNall RY et al. Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: implications for cell therapy of bone. Proc Natl Acad Sci USA 2002;99:8932-7.
    • (2002) Proc Natl Acad Sci USA , vol.99 , pp. 8932-8937
    • Horwitz, E.M.1    Gordon, P.L.2    Koo, W.K.3    Marx, J.C.4    Neel, M.D.5    McNall, R.Y.6
  • 7
    • 42749088243 scopus 로고    scopus 로고
    • Allogenic mesenchymal stem cell transplantation has a therapeutic effect in acute myocardial infarction in rats
    • Imanishi Y, Saito A, Komoda H, Kitagawa-Sakakida S, Miyagawa S, Kondoh H et al. Allogenic mesenchymal stem cell transplantation has a therapeutic effect in acute myocardial infarction in rats. J Mol Cell Cardiol 2008;44:662-71.
    • (2008) J Mol Cell Cardiol , vol.44 , pp. 662-671
    • Imanishi, Y.1    Saito, A.2    Komoda, H.3    Kitagawa-Sakakida, S.4    Miyagawa, S.5    Kondoh, H.6
  • 8
    • 55049135564 scopus 로고    scopus 로고
    • Allogenic injection of fetal membrane-derived mesenchymal stem cells induces therapeutic angiogenesis in a rat model of hind limb ischemia
    • Ishikane S, Ohnishi S, Yamahara K, Sada M, Harada K, Mishima K et al. Allogenic injection of fetal membrane-derived mesenchymal stem cells induces therapeutic angiogenesis in a rat model of hind limb ischemia. Stem Cells 2008;26:2625-33.
    • (2008) Stem Cells , vol.26 , pp. 2625-2633
    • Ishikane, S.1    Ohnishi, S.2    Yamahara, K.3    Sada, M.4    Harada, K.5    Mishima, K.6
  • 9
    • 40649113341 scopus 로고    scopus 로고
    • Transcriptional profiling of young and old mesenchymal stem cells in response to oxygen deprivation and reparability of the infarcted myocardium
    • Jiang S, Kh Haider H, Ahmed RP, Idris NM, Salim A, Ashraf M. Transcriptional profiling of young and old mesenchymal stem cells in response to oxygen deprivation and reparability of the infarcted myocardium. J Mol Cell Cardiol 2008;44: 582-96.
    • (2008) J Mol Cell Cardiol , vol.44 , pp. 582-596
    • Jiang, S.1    Kh Haider, H.2    Ahmed, R.P.3    Idris, N.M.4    Salim, A.5    Ashraf, M.6
  • 10
    • 77950548213 scopus 로고    scopus 로고
    • bFGF-modified BMMSCs enhance bone regeneration following distraction osteogenesis in rabbits
    • Jiang X, Zou S, Ye B, Zhu S, Liu Y, Hu J. bFGF-modified BMMSCs enhance bone regeneration following distraction osteogenesis in rabbits. Bone 2010;46:1156-61.
    • (2010) Bone , vol.46 , pp. 1156-1161
    • Jiang, X.1    Zou, S.2    Ye, B.3    Zhu, S.4    Liu, Y.5    Hu, J.6
  • 11
    • 0036651815 scopus 로고    scopus 로고
    • Maintenance of osteoblastic and adipocytic differentiation potential with age and osteoporosis in human marrow stromal cell cultures
    • Justesen J, Stenderup K, Eriksen EF, Kassem M. Maintenance of osteoblastic and adipocytic differentiation potential with age and osteoporosis in human marrow stromal cell cultures. Calcif Tissue Int 2002;71:36-44.
    • (2002) Calcif Tissue Int , vol.71 , pp. 36-44
    • Justesen, J.1    Stenderup, K.2    Eriksen, E.F.3    Kassem, M.4
  • 12
    • 56649095554 scopus 로고    scopus 로고
    • Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells
    • Kretlow JD, Jin YO, Liu W, Zhang WJ, Hong TH, Zhou G et al. Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol 2008;9:60
    • (2008) BMC Cell Biol , vol.9 , pp. 60
    • Kretlow, J.D.1    Jin, Y.O.2    Liu, W.3    Zhang, W.J.4    Hong, T.H.5    Zhou, G.6
  • 13
    • 33746547311 scopus 로고    scopus 로고
    • Transplanted mesenchymal stem cells accelerate glomerular healing in experimental glomerulonephritis
    • Kunter U, Rong S, Djuric Z, Boor P, Müller-Newen GD, Yu D et al. Transplanted mesenchymal stem cells accelerate glomerular healing in experimental glomerulonephritis. J Am Soc Nephrol 2006;17:2202-212.
    • (2006) J Am Soc Nephrol , vol.17 , pp. 2202-2212
    • Kunter, U.1    Rong, S.2    Djuric, Z.3    Boor, P.4    Müller-Newen, G.D.5    Yu, D.6
  • 14
    • 43049103438 scopus 로고    scopus 로고
    • Developmental Committee of the European Group for Blood and Marrow Transplantation. Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study
    • Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I et al. Developmental Committee of the European Group for Blood and Marrow Transplantation. Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study. Lancet 2008;371:1579-86.
    • (2008) Lancet , vol.371 , pp. 1579-1586
    • Le Blanc, K.1    Frassoni, F.2    Ball, L.3    Locatelli, F.4    Roelofs, H.5    Lewis, I.6
  • 15
    • 70349135107 scopus 로고    scopus 로고
    • Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium
    • Lee SK, Kim Y, Kim SS, Lee JH, Cho K, Lee SS et al. Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium. Proteomics 2009;9:4389-405.
    • (2009) Proteomics , vol.9 , pp. 4389-4405
    • Lee, S.K.1    Kim, Y.2    Kim, S.S.3    Lee, J.H.4    Cho, K.5    Lee, S.S.6
  • 16
    • 13844257282 scopus 로고    scopus 로고
    • Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR-gamma2 transcription factor and TGF-beta/BMP signalling pathways
    • Moerman EJ, Teng K, Lipschitz DA, Lecka-Czernik B. Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR-gamma2 transcription factor and TGF-beta/BMP signalling pathways. Aging Cell 2004;3:379-89.
    • (2004) Aging Cell , vol.3 , pp. 379-389
    • Moerman, E.J.1    Teng, K.2    Lipschitz, D.A.3    Lecka-Czernik, B.4
  • 17
    • 55049087497 scopus 로고    scopus 로고
    • Human bone marrow mesenchymal stem cells accelerate recovery of acute renal injury and prolong survival inmice
    • Morigi M, Introna M, Imberti B, Corna D, Abbate M, Rota C et al. Human bone marrow mesenchymal stem cells accelerate recovery of acute renal injury and prolong survival inmice, Stem Cells 2008;26:2075-82.
    • (2008) Stem Cells , vol.26 , pp. 2075-2082
    • Morigi, M.1    Introna, M.2    Imberti, B.3    Corna, D.4    Abbate, M.5    Rota, C.6
  • 18
    • 4544303726 scopus 로고    scopus 로고
    • Allogeneic bone marrow-derived mesenchymal stromal cells promote the regeneration of injured skeletal muscle without differentiation into myofibers
    • Natsu K, Ochi M, Mochizuki Y, Hachisuka H, Yanada S, Yasunaga Y. Allogeneic bone marrow-derived mesenchymal stromal cells promote the regeneration of injured skeletal muscle without differentiation into myofibers. Tissue Eng 2004;10:1093-112.
    • (2004) Tissue Eng , vol.10 , pp. 1093-1112
    • Natsu, K.1    Ochi, M.2    Mochizuki, Y.3    Hachisuka, H.4    Yanada, S.5    Yasunaga, Y.6
  • 19
    • 33746514768 scopus 로고    scopus 로고
    • Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a nonmyeloablative setting
    • Nauta AJ, Westerhuis G, Kruisselbrink AB, Lurvink EG, Willemze R, Fibbe WE. Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a nonmyeloablative setting. Blood 2006;108:2114-120.
    • (2006) Blood , vol.108 , pp. 2114-2120
    • Nauta, A.J.1    Westerhuis, G.2    Kruisselbrink, A.B.3    Lurvink, E.G.4    Willemze, R.5    Fibbe, W.E.6
  • 20
    • 57949106202 scopus 로고    scopus 로고
    • Survival of human mesenchymal stromal cells from bone marrow and adipose tissue after xenogenic transplantation in immunocompetent mice
    • Niemeyer P, Vohrer J, Schmal H, Kasten P, Fellenberg J, Suedkamp NP et al. Survival of human mesenchymal stromal cells from bone marrow and adipose tissue after xenogenic transplantation in immunocompetent mice. Cytotherapy 2008;10:784-95.
    • (2008) Cytotherapy , vol.10 , pp. 784-795
    • Niemeyer, P.1    Vohrer, J.2    Schmal, H.3    Kasten, P.4    Fellenberg, J.5    Suedkamp, N.P.6
  • 21
    • 0033926146 scopus 로고    scopus 로고
    • Is there a therapeutic opportunity to either prevent or treat osteopenic disorders by inhibiting marrow adipogenesis?
    • Nuttall ME, Gimble JM. Is there a therapeutic opportunity to either prevent or treat osteopenic disorders by inhibiting marrow adipogenesis? Bone 2000;27:177-84.
    • (2000) Bone , vol.27 , pp. 177-184
    • Nuttall, M.E.1    Gimble, J.M.2
  • 22
    • 70350125106 scopus 로고    scopus 로고
    • Cell surfacebound IL-1 alpha is an upstream regulator of the senescenceassociated IL-6/IL-8 cytokine network
    • Orjalo AV, Bhaumik D, Gengler BK, Scott GK, Campisi J. Cell surfacebound IL-1 alpha is an upstream regulator of the senescenceassociated IL-6/IL-8 cytokine network. Proc Natl Acad Sci USA 2009;106:17031-6.
    • (2009) Proc Natl Acad Sci USA , vol.106 , pp. 17031-17036
    • Orjalo, A.V.1    Bhaumik, D.2    Gengler, B.K.3    Scott, G.K.4    Campisi, J.5
  • 24
    • 34147174435 scopus 로고    scopus 로고
    • Although pig allogeneic mesenchymal stem cells are not immunogenic in vitro, intracardiac injection elicits an immune response in vivo
    • Poncelet AJ, Vercruysse J, Saliez A, Gianello P. Although pig allogeneic mesenchymal stem cells are not immunogenic in vitro, intracardiac injection elicits an immune response in vivo. Transplantation 2007;83:783-90.
    • (2007) Transplantation , vol.83 , pp. 783-790
    • Poncelet, A.J.1    Vercruysse, J.2    Saliez, A.3    Gianello, P.4
  • 26
    • 38649105374 scopus 로고    scopus 로고
    • Mesenchymal stem cell-mediated immunosuppression occurs via concerted action of chemokines and nitric oxide
    • Ren G, Zhang L, Zhao X, Xu G, Zhang Y, Roberts AI et al. Mesenchymal stem cell-mediated immunosuppression occurs via concerted action of chemokines and nitric oxide. Cell Stem Cell 2008;2:141-50.
    • (2008) Cell Stem Cell , vol.2 , pp. 141-150
    • Ren, G.1    Zhang, L.2    Zhao, X.3    Xu, G.4    Zhang, Y.5    Roberts, A.I.6
  • 28
    • 67649576912 scopus 로고    scopus 로고
    • Muscular dystrophy therapy by nonautologous mesenchymal stem cells:muscle regeneration without immunosuppression and inflammation
    • Shabbir A, Zisa D, Leiker M, Johnston C, Lin H, Lee T. Muscular dystrophy therapy by nonautologous mesenchymal stem cells:muscle regeneration without immunosuppression and inflammation. Transplantation 2009;87:1275-82.
    • (2009) Transplantation , vol.87 , pp. 1275-1282
    • Shabbir, A.1    Zisa, D.2    Leiker, M.3    Johnston, C.4    Lin, H.5    Lee, T.6
  • 29
    • 33845885144 scopus 로고    scopus 로고
    • Immune regulation by mesenchymal stem cells: two sides to the coin
    • Stagg J. Immune regulation by mesenchymal stem cells: two sides to the coin. Tissue Antigens 2007;69:1-9.
    • (2007) Tissue Antigens , vol.69 , pp. 1-9
    • Stagg, J.1
  • 30
    • 0347627149 scopus 로고    scopus 로고
    • Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells
    • Stenderup K, Justesen J, Clausen C, Kassem M. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone 2003;33:919-26.
    • (2003) Bone , vol.33 , pp. 919-926
    • Stenderup, K.1    Justesen, J.2    Clausen, C.3    Kassem, M.4
  • 31
    • 33744513898 scopus 로고    scopus 로고
    • Age-related impairment of mesenchymal progenitor cell function
    • Stolzing A, Scutt A. Age-related impairment of mesenchymal progenitor cell function. Aging Cell 2006;5:213-24.
    • (2006) Aging Cell , vol.5 , pp. 213-224
    • Stolzing, A.1    Scutt, A.2
  • 32
    • 39149093357 scopus 로고    scopus 로고
    • Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies
    • Stolzing E, Jones D, McGonagle A, Scutt A. Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies. Mech Ageing Dev 2008;129:163-73.
    • (2008) Mech Ageing Dev , vol.129 , pp. 163-173
    • Stolzing, E.1    Jones, D.2    McGonagle, A.3    Scutt, A.4
  • 33
    • 0033026491 scopus 로고    scopus 로고
    • Hydrogen peroxide induces necrosis, apoptosis, oncosis and apoptotic oncosis of mouse terminal proximal straight tubule cells
    • Takeda M, Shirato I, Kobayashi M, Endou H. Hydrogen peroxide induces necrosis, apoptosis, oncosis and apoptotic oncosis of mouse terminal proximal straight tubule cells. Nephron 1999;81:234-8.
    • (1999) Nephron , vol.81 , pp. 234-238
    • Takeda, M.1    Shirato, I.2    Kobayashi, M.3    Endou, H.4
  • 34
    • 0033005384 scopus 로고    scopus 로고
    • Hydrogen peroxide-induced apoptosis and necrosis in human lung fibroblasts: protective roles of glutathione
    • Teramoto S, Tomita T, Matsui H, Ohga E, Matsuse T, Ouchi Y. Hydrogen peroxide-induced apoptosis and necrosis in human lung fibroblasts: protective roles of glutathione. Jpn J Pharmacol 1999;79:33-40.
    • (1999) Jpn J Pharmacol , vol.79 , pp. 33-40
    • Teramoto, S.1    Tomita, T.2    Matsui, H.3    Ohga, E.4    Matsuse, T.5    Ouchi, Y.6
  • 35
    • 34247854809 scopus 로고    scopus 로고
    • Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury
    • Tögel F, Weiss K, Yang Y, Hu Z, Zhang P, Westenfelder C. Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury. Am J Physiol Renal Physiol 2007;292:1626-35.
    • (2007) Am J Physiol Renal Physiol , vol.292 , pp. 1626-1635
    • Tögel, F.1    Weiss, K.2    Yang, Y.3    Hu, Z.4    Zhang, P.5    Westenfelder, C.6
  • 36
    • 36549050333 scopus 로고    scopus 로고
    • A number of bone marrow mesenchymal stem cells but neither phenotype nor differentiation capacities changes with age of rats
    • Tokalov SV, Gruener S, Schindler S, Iagunov AS, Baumann M, Abolmaali ND. A number of bone marrow mesenchymal stem cells but neither phenotype nor differentiation capacities changes with age of rats. Mol Cells 2007;24:255-60.
    • (2007) Mol Cells , vol.24 , pp. 255-60
    • Tokalov, S.V.1    Gruener, S.2    Schindler, S.3    Iagunov, A.S.4    Baumann, M.5    Abolmaali, N.D.6
  • 37
    • 0035955303 scopus 로고    scopus 로고
    • Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF
    • Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E et al. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun 2001;288:413-19.
    • (2001) Biochem Biophys Res Commun , vol.288 , pp. 413-419
    • Tsutsumi, S.1    Shimazu, A.2    Miyazaki, K.3    Pan, H.4    Koike, C.5    Yoshida, E.6
  • 39
    • 62749084481 scopus 로고    scopus 로고
    • Effect of transplanted mesenchymal stem cells from rats of different ages on the improvement of heart function after acute myocardial infarction
    • Wang YQ, Wang M, Zhang P, Song JJ, Li YP, Hou SH et al. Effect of transplanted mesenchymal stem cells from rats of different ages on the improvement of heart function after acute myocardial infarction. Chin Med J 2008;121:2290-8.
    • (2008) Chin Med J , vol.121 , pp. 2290-2298
    • Wang, Y.Q.1    Wang, M.2    Zhang, P.3    Song, J.J.4    Li, Y.P.5    Hou, S.H.6
  • 40
    • 43049162965 scopus 로고    scopus 로고
    • Age-related intrinsic changes in human bone-marrow-derived mesenchymal stem cells and their differentiation to osteoblasts
    • Zhou S, Greenberger JS, Epperly MW, Goff JP, Adler C, Leboff MS et al. Age-related intrinsic changes in human bone-marrow-derived mesenchymal stem cells and their differentiation to osteoblasts. Aging Cell 2008;7:335-43.
    • (2008) Aging Cell , vol.7 , pp. 335-343
    • Zhou, S.1    Greenberger, J.S.2    Epperly, M.W.3    Goff, J.P.4    Adler, C.5    Leboff, M.S.6
  • 41
    • 44249090447 scopus 로고    scopus 로고
    • Extracellular signal-regulated kinase activation mediates mitochondrial dysfunction and necrosis induced by hydrogen peroxide in renal proximal tubular cells
    • Zhuang S, Kinsey GR, Yan Y, Han J, Schnellmann RG. Extracellular signal-regulated kinase activation mediates mitochondrial dysfunction and necrosis induced by hydrogen peroxide in renal proximal tubular cells. J Pharmacol Exp Ther 2008;325:732-40.
    • (2008) J Pharmacol Exp Ther , vol.325 , pp. 732-740
    • Zhuang, S.1    Kinsey, G.R.2    Yan, Y.3    Han, J.4    Schnellmann, R.G.5
  • 42
    • 70449705826 scopus 로고    scopus 로고
    • Vascular endothelial growth factor (VEGF) as a key therapeutic trophic factor in bone marrow mesenchymal stem cell-mediated cardiac repair
    • Zisa D, Shabbir A, Suzuki G, Lee T. Vascular endothelial growth factor (VEGF) as a key therapeutic trophic factor in bone marrow mesenchymal stem cell-mediated cardiac repair. Biochem Biophys Res Commun 2009;390:834-8.
    • (2009) Biochem Biophys Res Commun , vol.390 , pp. 834-838
    • Zisa, D.1    Shabbir, A.2    Suzuki, G.3    Lee, T.4


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