Molecular characterization of genes encoding trypsin-like enzymes from Aedes aegypti larvae and identification of digestive enzymes

Gene

Volumn 489, Issue 2, 2011, Pages 70-75

  Soares, Tatiane S ^a   Watanabe, Renata M O ^a   Lemos, Francisco J A ^b   Tanaka, Aparecida S ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO   (Brazil)

Author keywords

Aedes; BPTI; Larvae; Mosquito; Serine protease inhibitor; Trypsin

Indexed keywords

AMINO ACID; COMBIZYM; TRYPTASE;

AEDES AEGYPTI; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME PURIFICATION; FEMALE; GENETIC CODE; LARVA; MIDGUT; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

AEDES; AMINO ACID SEQUENCE; ANIMALS; DIGESTION; DIGESTIVE SYSTEM; GENE EXPRESSION; LARVA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, PROTEIN; TRYPSIN;

AEDES AEGYPTI;

EID: 80054874867     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2011.08.018     Document Type: Article

References (30)

1. Azzolini S.S., et al. The role of HiTI, a serine protease inhibitor from Haematobia irritans irritans (Diptera: Muscidae) in the control of fly and bacterial proteases. Exp. Parasitol. 2005, 111:30-36.
2. Barillas-Mury C., Wells M.A. Cloning and sequencing of the blood meal-induced late trypsin gene from the mosquito Aedes aegypti and characterization of the upstream regulatory region. Insect. Mol. Biol. 1993, 2:7-12.
3. Barillas-Mury C.V., Noriega F.G., Wells M.A. Early trypsin activity is part of the signal transduction system that activates transcription of the late trypsin gene in the midgut of the mosquito, Aedes aegypti. Insect. Biochem. Mol. Biol. 1995, 25:241-246.
4. Blum H., Beier H., Gross H.J. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Eletrophoresis 1987, 8:93-99.
5. Craik C.S., et al. Redesigning trypsin: alteration of substrate specificity. Science 1985, 228:291-297.
6. Edman P., Begg G. A protein sequenator. Eur. J. Biochem. 1967, 1:80-91.
7. Halstead S. Global perspective on dengue research. Dengue Bull. 2000, 24:77-82.
8. Horler E., Briegel H. Chymotrypsin inhibitors in mosquitoes: activity profile during development and after blood feeding. Arch. Insect Biochem. Physiol. 1997, 36:315-333.
9. Isoe J., Zamora J., Miesfeld R.L. Molecular analysis of the Aedes aegypti carboxypeptidase gene family. Insect. Biochem Mol. Biol. 2009, 39:68-73.
10. Jiang Q., et al. cDNA cloning and pattern of expression of an adult, female-specific chymotrypsin from Aedes aegypti midgut. Insect Biochem. Mol. Biol. 1997, 27:283-289.
11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
12. Lam W., Coast G.M., Rayne R.C. Characterisation of multiple trypsins from the midgut of Locusta migratoria. Insect Biochem. Mol. Biol. 2000, 30:85-94.
13. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
14. Lopes A.R., Terra W.R. Purification, properties and substrate specificity of a digestive trypsin from Periplaneta americana (Dictyoptera) adults. Insect Biochem. Mol. Biol. 2003, 33:407-415.
15. Lopes A.R., Juliano M.A., Marana S.R., Juliano L., Terra W.R. Substrate specificity of insect trypsins and the role of their subsites in catalysis. Insect Biochem. Mol. Biol. 2006, 36:130-140.
16. Lu S.J., et al. Reevaluation of the role of early trypsin activity in the transcriptional activation of the late trypsin gene in the mosquito Aedes aegypti. Insect Biochem. Mol. Biol. 2006, 36:336-343.
17. Mackenzie J.S., Gubler D.J., Petersen L.R. Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat. Med. 2004, 10:S98-S109.
18. Mesquita-Rodrigues C., Saboia-Vahia L., Cuervo P., Masini d'Avila Levy C., Alves Honorio N., Domont G.B., Batista de Jesus J. Expression of trypsin-like serine peptidases in pre-imaginal stages of Aedes aegypti (Diptera: Culicidae). Arch. Insect Biochem. Physiol. 2011, 76:223-235.
19. Moll R.M., et al. Meconial peritrophic membranes and the fate of midgut bacteria during mosquito (Diptera: Culicidae) metamorphosis. J. Med. Entomol. 2001, 38:29-32.
20. Morlais I., et al. A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti. Mol. Genet. Genomics 2003, 269:753-764.
21. Nene V., et al. Genome sequence of Aedes aegypti, a major arbovirus vector. Science 2007, 316:1718-1723.
22. Noriega F.G., Wells M.A. A molecular view of trypsin synthesis in the midgut of Aedes aegypti. J. Insect Physiol. 1999, 45:613-620.
23. Tanaka A.S., et al. Functional phage display of leech-derived tryptase inhibitor (LDTI): construction of a library and selection of thrombin inhibitors. FEBS Lett. 1999, 458:11-16.
24. Tauil P.L. Critical aspects of dengue control in Brazil. Cad. Saude Publica. 2002, 18:867-871.
25. Terra W.R., Ferreira C. Insect digestive enzymes: properties, compartmentalization and function. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 1994, 109:1-62.
26. Venancio T.M., et al. The Aedes aegypti larval transcriptome: a comparative perspective with emphasis on trypsins and the domain structure of peritrophins. Insect Mol. Biol. 2009, 18:33-44.
27. Volpicella M., et al. Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors. Eur. J. Biochem. 2003, 270:10-19.
28. Watanabe R.M., et al. A novel trypsin Kazal-type inhibitor from Aedes aegypti with thrombin coagulant inhibitory activity. Biochimie 2010, 92:933-939.
29. Yang Y.J., Davies D.M. Digestive enzymes in the excreta of Aedes aegypti larvae. J. Insect Physiol. 1971, 17:2119-2123.
30. Yang Y.J., Davies D.M. Trypsin and chymotrypsin during metamorphosis in Aedes aegypti and properties of the chymotrypsin. J. Insect Physiol. 1971, 17:117-131.