Multicolor BiFC Analysis of G Protein βγ Complex Formation and Localization

Methods in Molecular Biology

Volumn 756, Issue , 2011, Pages 229-243

  Hynes, Thomas R ^a   Yost, Evan A ^a   Yost, Stacy M ^a   Berlot, Catherine H ^a  

^a WEIS CENTER FOR RESEARCH   (United States)

Author keywords

Cyan fluorescent protein; Fluorescence microscopy; G protein complex; G protein coupled receptor; Heterotrimeric G protein; Live cell imaging; Multicolor bimolecular fluorescence complementation; Spectrofluorometer; Subcellular targeting; Yellow fluorescent protein

Indexed keywords

CYAN FLUORESCENT PROTEIN; G PROTEIN BETA GAMMA; G-PROTEIN BETA GAMMA; GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN BETA SUBUNIT; GUANINE NUCLEOTIDE BINDING PROTEIN GAMMA SUBUNIT; HYBRID PROTEIN; PHOTOPROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL LINE; CELL STRUCTURE; CHEMICAL STRUCTURE; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SPECTROFLUOROMETRY; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; CELLULAR STRUCTURES; GREEN FLUORESCENT PROTEINS; GTP-BINDING PROTEIN BETA SUBUNITS; GTP-BINDING PROTEIN GAMMA SUBUNITS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TRANSFECTION;

EID: 80054747851     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-160-4_12     Document Type: Chapter

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