Proteomics method for identification of pseudopodium phosphotyrosine proteins

Methods in Molecular Biology

Volumn 757, Issue , 2011, Pages 349-365

  Wang, Yingchun ^a   Klemke, Richard L ^b  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cell body; Cell migration; Cytoskeleton; Immunoprecipitation; Kinases; Phosphotyrosine; Proteomics; Pseudopodium; Signal transduction

Indexed keywords

PHOSPHOTYROSINE; PROTEIN;

ANIMAL; ARTICLE; CELL MOTION; CELL STRAIN 3T3; CELL STRAIN COS1; CERCOPITHECUS; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; PHYSIOLOGY; PROTEOMICS; PSEUDOPODIUM;

ANIMALS; CELL MOVEMENT; CERCOPITHECUS AETHIOPS; COS CELLS; MICE; NIH 3T3 CELLS; PHOSPHOTYROSINE; PROTEINS; PROTEOMICS; PSEUDOPODIA;

EID: 80054745728     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-166-6_21     Document Type: Article

References (13)

1. Hunter, T. (2000) Signaling-2000 and beyond. Cell 100, 113-127.
2. Linding, R., Jensen, L. J., Ostheimer, G. J., van Vugt, M. A., Jorgensen, C., Miron, I. M., et al. (2007) Systematic discovery of in vivo phosphorylation networks. Cell 129, 1415-1426. (Pubitemid 46962092)
3. Miller, M. L., Jensen, L. J., Diella, F., Jorgensen, C., Tinti, M., Li, L., et al. (2008) Linear motif atlas for phosphorylation-dependent signaling. Sci. Signal. 1, ra2.
4. Pawson, T., and Kofler, M. (2009) Kinome signaling through regulated protein-protein interactions in normal and cancer cells. Curr. Opin. Cell Biol. 21, 147-153.
5. Pawson, T., and Nash, P. (2003) Assembly of cell regulatory systems through protein interaction domains. Science 300, 445-452. (Pubitemid 36444318)
6. Cho, S. Y., and Klemke, R. L. (2002) Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold. J Cell. Biol. 156, 725-736. (Pubitemid 34839887)
7. Wang, Y., Ding, S. J., Wang, W., Jacobs, J. M., Qian, W. J., Moore, R. J., et al. (2007) Profiling signaling polarity in chemotactic cells. Proc. Natl. Acad. Sci. USA. 104, 8328-8333. (Pubitemid 47175487)
8. Cohen, P. (2000) The regulation of protein function by multisite phosphorylation-a 25 year update. Trends Biochem. Sci. 25, 596-601.
9. Mann, M., Ong, S. E., Gronborg, M., Steen, H., Jensen, O. N., and Pandey, A. (2002) Analysis of protein phosphorylation using mass spectrometry: Deciphering the phosphoproteome. Trends Biotechnol. 20, 261-268. (Pubitemid 34451062)
10. Washburn, M. P., Wolters, D., and Yates, J. R., 3rd. (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat. Biotechnol. 19, 242-247. (Pubitemid 32220565)
11. Ge, L., Ly, Y., Hollenberg, M., and DeFea, K. (2003) A beta-arrestin-dependent scaffold is associated with prolonged MAPK activation in pseudopodia during protease-activated receptor-2-induced chemotaxis. J. Biol. Chem. 278, 34418-34426. (Pubitemid 37553288)
12. Howe, A. K., Baldor, L. C., and Hogan, B. P. (2005) Spatial regulation of the cAMPdependent protein kinase during chemotactic cell migration. Proc. Natl. Acad. Sci. USA. 102, 14320-14325. (Pubitemid 41429696)
13. Jia, Z., Barbier, L., Stuart, H., Amraei, M., Pelech, S., Dennis, J. W., et al. (2005) Tumor cell pseudopodial protrusions. Localized signaling domains coordinating cytoskeleton remodeling, cell adhesion, glycolysis, RNA translocation, and protein translation. J. Biol. Chem. 280, 30564-30573. (Pubitemid 41216241)