Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold

Journal of Cell Biology

Volumn 156, Issue 4, 2002, Pages 725-736

  Cho, Samuel Y ^a   Klemke, Richard L ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Cell migration; Chemotaxis; Pseudopodium; Rac GTPase; Signal transduction

Indexed keywords

CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; FOCAL ADHESION KINASE; PROTEIN; PROTEIN CRK; RAC PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MIGRATION; CELL POLARITY; CHEMOKINESIS; CHEMOTAXIS; COMPLEX FORMATION; ENZYME ACTIVATION; ENZYME ACTIVITY; NONHUMAN; POSITIVE FEEDBACK; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PSEUDOPODIUM; RAT; SIGNAL TRANSDUCTION;

3T3 CELLS; ANIMALS; CDC42 GTP-BINDING PROTEIN; CELL POLARITY; CERCOPITHECUS AETHIOPS; CHEMOTACTIC FACTORS; CHEMOTAXIS; COS CELLS; CRK-ASSOCIATED SUBSTRATE PROTEIN; ENZYME ACTIVATION; LYSOPHOSPHOLIPIDS; MICE; PHOSPHOPROTEINS; PROTEIN KINASES; PROTEINS; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-CBL; PROTO-ONCOGENE PROTEINS C-CRK; PSEUDOPODIA; RAC GTP-BINDING PROTEINS; RAC1 GTP-BINDING PROTEIN; RETINOBLASTOMA-LIKE PROTEIN P130; RHO GTP-BINDING PROTEINS; UBIQUITIN-PROTEIN LIGASES;

ANIMALIA;

EID: 0037128213     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200111032     Document Type: Article

References (37)

1. A role for Cdc42 in macrophage chemotaxis
2. Signal transduction and signal modulation by cell adhesion receptors: The role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins
3. Gelsolin is a downstream effector of Rat for fibroblast motility
4. Regulation of protrusion shape and adhesion to the substratum during chemotactic responses of mammalian carcinoma cells
5. Regulation of cell contraction and membrane ruffling by distinct signals in migratory cells
6. Extracellular-regulated kinase activation and CAS/Crk coupling regulate cell migration and suppress apoptosis during invasion of the exrracellular matrix
7. Ca21-dependent myosin II activation is required for uropod retraction during neutrophil migration
8. Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCz
9. Lysophospholipid receptors
10. cas as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST
11. Caldemon inhibits non-muscle cell contractility and interferes with the formation of focal adhesions
12. Localization of the G protein bg complex in living cells during chemotaxis
13. Inhibition of cell migration by Ab1 family tyrosine kinases through uncoupling of Crk-CAS complexes
14. Rac recruits high-affinity integrin avb3 to lamellipodia in endothelial cell migration
15. CAS/Crk coupling serves as a "molecular switch" for induction of cell migration
16. Localized Rac activation dynamics visualized in living cells
17. Cell migration: A physically integrated molecular process
18. Differential dynamics of a5 integrin, paxillin, and a-actinin during formation and disassembly of adhesions in migrating cells
19. cas
20. cas
21. Membrane raft microdomains mediate front-rear polarity in migrating cells
22. Structural requirement of CRK SH2 region for binding to phosphotyrosine containing proteins
23. Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases
24. Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape
25. Chemoattractant-mediated transient activation and membrane localization of Akt/PKB is required for efficient chemotaxis to cAMP in Dictyostelium
26. Rho GTPases control polarity, protrusion, and adhesion during cell movement
27. The small GTPases Ra1A targets filamin to induce filopodia
28. A cell's sense of direction
29. G protein signaling events are activated at the leading edge of chemotactic cells
30. The small GTP-binding protein Rac regulates growth factor-induced membrane ruffling
31. Integrin cytoskeletal interactions in migrating fibroblasts are dynamic and asymmetrical, and regulated
32. Rho GTPases: Signaling, migration, and invasion
33. Polarization of chemoatrractant receptor signaling during neutrophil chemotaxis
34. Focal adhesion motility revealed in stationary fibroblasts
35. Tyrosine phosphorylarion of Crk-associated substrates by focal adhesion kinase
36. Both the SH2 and SH3 domains of Crk protein required for neuronal differentiation of PC12 cells
37. Induction of p130CAS signaling complex formation upon inregrin-mediared cell adhesion: A role for src family kinases