Lipid nanoparticle purification by Spin Centrifugation-Dialysis (SCD): A facile and high-throughput approach for small scale preparation of siRNA-lipid complexes

International Journal of Pharmaceutics

Volumn 420, Issue 1, 2011, Pages 118-121

  Mihaila, Radu ^a   Chang, Silvia ^a   Wei, Alan T ^a   Hu, Zhi Y ^a   Ruhela, Dipali ^a   Shadel, Timothy R ^a   Duenwald, Sven ^a   Payson, Elmer ^a   Cunningham, James J ^b   Kuklin, Nelly ^a   Mathre, David J ^b  

^a Sirna Therapeutics Inc   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Encapsulation; Lipid Nanoparticle (LNP); Liposome; siRNA; Spin Centrifugation Dialysis (SCD); Tangential Flow Filtration (TFF)

Indexed keywords

LIPID NANOPARTICLE; NANOPARTICLE; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CENTRIFUGATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG SYNTHESIS; FILTRATION; GENE SILENCING; IN VITRO STUDY; IN VIVO STUDY; INTERMETHOD COMPARISON; LIPID COMPOSITION; MOUSE; NANOENCAPSULATION; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; SINGLE DRUG DOSE; SPIN CENTRIFUGATION DIALYSIS; TANGENTIAL FLOW FILTRATION;

ANIMALS; BUFFERS; CELL LINE, TUMOR; CENTRIFUGATION; DIALYSIS; DOWN-REGULATION; EQUIPMENT DESIGN; FILTRATION; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROGEN-ION CONCENTRATION; LIPIDS; LUCIFERASES; MICE; MICE, INBRED C57BL; MINIATURIZATION; NANOPARTICLES; NANOTECHNOLOGY; RNA INTERFERENCE; RNA, SMALL INTERFERING; TIME FACTORS; TRANSFECTION;

EID: 80054707756     PISSN: 03785173     EISSN: 18733476     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2011.08.017     Document Type: Article

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