Expression of a cholera toxin B subunit-neutralizing epitope of the porcine epidemic diarrhea virus fusion gene in transgenic lettuce (Lactuca sativa L.)

Molecular Biotechnology

Volumn 48, Issue 3, 2011, Pages 201-209

  Huy, Nguyen Xuan ^a   Yang, Moon Sik ^a,b   Kim, Tae Geum ^a  

^a Chonbuk National University   (South Korea)

^b Korea Basic Science Institute (KBSI)   (South Korea)

Author keywords

Cholera toxin B subunit; COE; Edible vaccine; PEDV; Transgenic lettuce

Indexed keywords

CHOLERA TOXIN B SUBUNIT; COE; EDIBLE VACCINES; PEDV; TRANSGENICS;

ANTIBODIES; ANTIGENS; BIOSYNTHESIS; DISEASE CONTROL; OLIGOMERS; POLYMERASE CHAIN REACTION; TISSUE; TOXIC MATERIALS; VIRUSES;

GENE EXPRESSION;

CHOLERA TOXIN B SUBUNIT; EPITOPE; GANGLIOSIDE GM1; GENOMIC DNA; OLIGOMER; CHOLERA TOXIN; EDIBLE VACCINE; RECOMBINANT PROTEIN; VIRUS FUSION PROTEIN;

ARTICLE; CONTROLLED STUDY; FUSION GENE; GENE EXPRESSION; LETTUCE; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PORCINE EPIDEMIC DIARRHEA VIRUS; RHIZOBIUM; SYNTHESIS; TRANSGENIC CROP; WESTERN BLOTTING; BIOSYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; IMMUNOBLOTTING; IMMUNOLOGY; METABOLISM; MOLECULAR CLONING; PROTEIN ENGINEERING; TRANSGENIC PLANT;

AGROBACTERIUM; LACTUCA; LACTUCA SATIVA; PORCINE EPIDEMIC DIARRHEA VIRUS;

BLOTTING, NORTHERN; BLOTTING, WESTERN; CHOLERA TOXIN; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; G(M1) GANGLIOSIDE; IMMUNOBLOTTING; LETTUCE; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; PORCINE EPIDEMIC DIARRHEA VIRUS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; VACCINES, EDIBLE; VIRAL FUSION PROTEINS;

EID: 80054014131     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-010-9359-1     Document Type: Article

References (55)

1. Cavanagh, D. (2005). Coronaviridae: A review of coronaviruses and toroviruses (pp. 1-54). Basel: Birkhäuser Verlag.
2. Cavanagh, D., Brian, D. A., Brinton, M. A., Enjuanes, L., Holmes, K. V., Horzinek, M. C., et al. (1993). The Coronaviridae now comprises two genera, coronavirus and torovirus: Report of the Coronaviridae Study Group. Advances in Experimental Medicine and Biology, 342, 255-257. (Pubitemid 24095139)
3. Pensaert, M. B., & de Bouck, P. (1978). A new coronavirus-like particle associated with diarrhea in swine. Archives of Virology, 58, 243-247. (Pubitemid 9047249)
4. Chasey, D., & Cartwright, S. F. (1978). Virus-like particles associated with porcine epidemic diarrhoea. Research in Veterinary Science, 25, 255-256. (Pubitemid 9033920)
5. Turgeon, D. C., Morin, M., Jolette, J., Higgins, R., Marsolais, G., & DiFranco, E. (1980). Coronavirus-like particles associated with diarrhea in baby pigs in Quebec. Canadian Veterinary Journal, 21, 100-101.
6. Horvath, I., & Mocsari, E. (1981). Ultrastructural changes in the small intestinal epithelium of suckling pigs affected with a transmissible gastroenteritis (TGE)-like disease. Archives of Virology, 68, 103-113. (Pubitemid 11076100)
7. Pospischil, A., Hess, R. G., & Bachmann, P. A. (1981). Light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic diarrhoea virus (EVD): Comparison with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections. Zentralbl Veterinarmed B, 28, 564-577.
8. Takahashi, K., Okada, K., & Ohshima, K. (1983). An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan. Nippon Juigaku Zasshi, 45, 829-832.
9. Qinghua, C. (1992). Investigation on epidemic diarrhea in pigs in Qinghai region. Qinghai Xuma Shaoyi Zazhi, 22, 22-23.
10. Chae, C., Kim, O., Choi, C., Cho, W. S., Kim, J., & Tai, J. H. (2000). Prevalence of porcine epidemic diarrhoea virus and transmissible gastroenteritis virus infection in Korean pigs. The Veterinary Record, 147, 606-608.
11. Puranaveja, S., Poolperm, P., Lertwatcharasakul, P., Kesdaengsakonwut, S., Boonsoongnern, A., Urairong, K., et al. (2009). Chinese-like strain of porcine epidemic diarrhea virus, Thailand. Emerging Infectious Diseases, 15, 1112-1115.
12. Duarte, M., & Laude, H. (1994). Sequence of the spike protein of the porcine epidemic diarrhoea virus. Journal of General Virology, 75(Pt 5), 1195-1200. (Pubitemid 24143167)
13. Kocherhans, R., Bridgen, A., Ackermann, M., & Tobler, K. (2001). Completion of the porcine epidemic diarrhoea coronavi-rus (PEDV) genome sequence. Virus Genes, 23, 137-144. (Pubitemid 33020687)
14. Egberink, H. F., Ederveen, J., Callebaut, P., & Horzinek, M. C. (1988). Characterization of the structural proteins of porcine epizootic diarrhea virus, strain CV777. American Journal of Veterinary Research, 49, 1320-1324.
15. Yeo, S. G., Hernandez, M., Krell, P. J., & Nagy, E. E. (2003). Cloning and sequence analysis of the spike gene of porcine epidemic diarrhea virus Chinju99. Virus Genes, 26, 239-246. (Pubitemid 36898143)
16. Chang, S. H., Bae, J. L., Kang, T. J., Kim, J., Chung, G. H., Lim, C. W., et al. (2002). Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus. Molecules and Cells, 14, 295-299. (Pubitemid 41594986)
17. Daniell, H., Streatfield, S. J., & Wycoff, K. (2001). Medical molecular farming: Production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends in Plant Science, 6, 219-226.
18. Hellwig, S., Drossard, J., Twyman, R. M., & Fischer, R. (2004). Plant cell cultures for the production of recombinant proteins. Nature Biotechnology, 22, 1415-1422. (Pubitemid 39482861)
19. Rigano, M. M., & Walmsley, A. M. (2005). Expression systems and developments in plant-made vaccines. Immunology and Cell Biology, 83, 271-277. (Pubitemid 40799555)
20. Walmsley, A. M., & Arntzen, C. J. (2000). Plants for delivery of edible vaccines. Current Opinion in Biotechnology, 11, 126-129. (Pubitemid 30182304)
21. Doran, P. M. (2006). Foreign protein degradation and instability in plants and plant tissue cultures. Trends in Biotechnology, 24, 426-432. (Pubitemid 44223821)
22. Holmgren, J., Harandi, A. M., & Czerkinsky, C. (2003). Mucosal adjuvants and anti-infection and anti-immunopathology vaccines based on cholera toxin, cholera toxin B subunit and CpG DNA. Expert Review of Vaccines, 2, 205-217. (Pubitemid 36559926)
23. Sixma, T. K., Pronk, S. E., Kalk, K. H., Wartna, E. S., van Zanten, B. A., Witholt, B., et al. (1991). Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli. Nature, 351, 371-377. (Pubitemid 21896597)
24. Merritt, E. A., Sarfaty, S., van den Akker, F., L'Hoir, C., Martial, J. A., & Hol, W. G. (1994). Crystal structure of cholera toxin B-pentamer bound to receptor GM1 pentasaccharide. Protein Science, 3, 166-175. (Pubitemid 24086350)
25. Zhang, R. G., Scott, D. L., Westbrook, M. L., Nance, S., Span-gler, B. D., Shipley, G. G., et al. (1995). The three-dimensional crystal structure of cholera toxin. Journal of Molecular Biology, 251, 563-573.
26. Fujinaga, Y. (2006). Transport of bacterial toxins into target cells: Pathways followed by cholera toxin and botulinum progenitor toxin. Journal of Biochemistry, 140, 155-160. (Pubitemid 44618900)
27. Rask, C., Fredriksson, M., Lindblad, M., Czerkinsky, C., & Holmgren, J. (2000). Mucosal and systemic antibody responses after peroral or intranasal immunization: Effects of conjugation to enterotoxin B subunits and/or of co-administration with free toxin as adjuvant. APMIS, 108, 178-186. (Pubitemid 30158997)
28. Sanchez, J., Johansson, S., Lowenadler, B., Svennerholm, A. M., & Holmgren, J. (1990). Recombinant cholera toxin B subunit and gene fusion proteins for oral vaccination. Research in Microbiology, 141, 971-979.
29. Yuki, Y., & Kiyono, H. (2003). New generation of mucosal adjuvants for the induction of protective immunity. Reviews in Medical Virology, 13, 293-310. (Pubitemid 37082192)
30. McKenzie, S., & Halseu, J. (1984). Cholera toxin B subunit as a carrier protein to stimulate a mucosal immune response. Journal of Immunology, 133, 1818-1824. (Pubitemid 14035270)
31. Arakawa, T., Yu, J., Chong, D. K., Hough, J., Engen, P. C., & Langridge, W. H. (1998). A plant-based cholera toxin B subunit-insulin fusion protein protects against the development of autoimmune diabetes. Nature Biotechnology, 16, 934-938. (Pubitemid 28483621)
32. Befus, N., & Langridge, W. H. R. (2000). Plant-based cholera toxin B subunit-insulin/GAD fusion proteins suppress the development of autoimmune diabetes. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz, 43, 116-120.
33. Kim, T. G., & Langridge, W. H. (2003). Assembly of cholera toxin B subunit full-length rotavirus NSP4 fusion protein oligo-mers in transgenic potato. Plant Cell Reports, 21, 884-890. (Pubitemid 36708502)
34. Li, D., O'Leary, J., Huang, Y., Huner, N. P., Jevikar, A. M., & Ma, S. (2006). Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants. Plant Cell Reports, 25, 417-424.
35. Nozoye, T., Takaiwa, F., Tsuji, N., Yamakawa, T., Arakawa, T., Hayashi, Y., et al. (2009). Production of Ascaris suum As14 protein and its fusion protein with cholera toxin B subunit in rice seeds. Journal of Veterinary Medical Science, 71, 995-1000.
36. Bae, J. L., Lee, J. G., Kang, T. J., Jang, Y. S., & Yang, M. S. (2003). Induction of antigen-specific systemic and mucosal immune responses by feeding animals transgenic plants expressing the antigen. Vaccine, 21, 4052-4058.
37. Kang, T. J., Kang, K. H., Kim, J. A., Kwon, T. H., Jang, Y. S., & Yang, M. S. (2004). High-level expression of the neutralizing epitope of porcine epidemic diarrhea virus by a tobacco mosaic virus-based vector. Protein Expression and Purification, 38, 129-135.
38. Kang, T. J., Kim, Y. S., Jang, Y. S., & Yang, M. S. (2005). Expression of the synthetic neutralizing epitope gene of porcine epidemic diarrhea virus in tobacco plants without nicotine. Vaccine, 23, 2294-2297.
39. Kang, T. J., Han, S. C., Yang, M. S., & Jang, Y. S. (2006). Expression of synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat-labile enterotoxin in tobacco plants. Protein Expression and Purification, 46, 16-22.
40. Oszvald, M., Kang, T. J., Tomoskozi, S., Tamas, L., Kim, T. G., & Yang, M. S. (2007). Expression of a synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat labile enterotoxin in rice endosperm. Molecular Biotechnology, 35, 215-223. (Pubitemid 47233920)
41. Huy, N. X., Kim, Y. S., Jun, S. C., Jin, Z., Park, S. M., Yang, M. S., et al. (2009). Production of a heat-labile enterotoxin B sub-unit-porcine epidemic diarrhea virus-neutralizing epitope fusion protein in transgenic lettuce (Lactuca sativa). Biotechnology and Bioprocess Engineering, 14, 731-737.
42. Lelivelt, C. L., McCabe, M. S., Newell, C. A., Desnoo, C. B., van Dun, K. M., Birch-Machin, I., et al. (2005). Stable plastid transformation in lettuce (Lactuca sativa L.). Plant Molecular Biology, 58, 763-774. (Pubitemid 41481804)
43. Kang, T. J., Loc, N. H., Jang, M. O., & Yang, M. S. (2004). Modification of the cholera toxin B subunit coding sequence to enhance expression in plants. Molecular Breeding, 13, 143-153.
44. Kozak, M. (1989). The scanning model for translation: An update. Journal of Cell Biology, 108, 229-241. (Pubitemid 19065830)
45. Van Haute, E., Joos, H., Maes, M., Warren, G., Van Montagu, M., & Schell, J. (1983). Integeneric transfer and exchange recombination of restriction fragments cloned in pBR322: A novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO Journal, 2, 411-417.
46. Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
47. Lehrach, H., Diamound, D., Wozney, J. M., & Boedtker, H. (1977). RNA molecular weight determinations by gel electro-phoresis under denaturing conditions, a critical reexamination. Biochemistry, 16, 4743-4751. (Pubitemid 8205616)
48. Arakawa, T., Chong, D. K., Merritt, J. L., & Langridge, W. H. (1997). Expression of cholera toxin B subunit oligomers in transgenic potato plants. Transgenic Research, 6, 403-413. (Pubitemid 27526605)
49. Kang, T. J., Kwon, T. H., Kim, T. G., Loc, N. H., & Yang, M. S. (2003). Comparing constitutive promoters using CAT activity in transgenic tobacco plants. Molecules and Cells, 16, 117-122.
50. Peach, C., & Velten, J. (1991). Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Molecular Biology, 17, 49-60.
51. Baulocombe, D. (2004). RNA silencing in plants. Nature, 431, 356-363.
52. Hondred, D., Walker, J. M., Mathews, D. E., & Vierstra, D. (1999). Use of ubiquitin fusions to augment protein expression in transgenic plants. Plant Physiology, 119, 713-723. (Pubitemid 29384562)
53. Kang, T. J., Kim, B. G., Yang, J. Y., & Yang, M. S. (2006). Expression of a synthetic cholera toxin B subunit in tobacco using ubiquitin promoter and bar gene as a selectable marker. Molecular Biotechnology, 32, 93-100.
54. Daniell, H., Lee, S. B., Panchal, T., & Wiebe, P. O. (2001). Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco. Journal of Molecular Biology, 311, 1001-1009. (Pubitemid 32803716)
55. Kim, Y. S., Kim, B. G., Kim, T. G., Kang, T. J., & Yang, M. S. (2006). Expression of a cholera toxin B subunit in transgenic lettuce (Lactuca sativa L.) using Agrobacterium-mediated transformation system. Plant Cell, Tissue and Organ Culture, 87, 203-210.