A novel approach of protein immobilization for protein chips using an oligo-cysteine tag

Journal of Proteome Research

Volumn 5, Issue 9, 2006, Pages 2144-2151

  Ichihara, Teruhisa ^a   Akada, Junko K ^b   Kamei, Shuichi ^a   Ohshiro, Saemi ^b   Sato, Daisuke ^b   Fujimoto, Masanori ^b   Kuramitsu, Yasuhiro ^b   Nakamura, Kazuyuki ^b  

^a Toyo Kohan Co   (Japan)

^b YAMAGUCHI UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Cysteine tag; DLC; Maleimide group; Maleimide coated substrate; Orientation; Protein chip; Thiol group

Indexed keywords

AMINO ACID; CARBON; CYSTEINE DERIVATIVE; DNA FRAGMENT; ENHANCED GREEN FLUORESCENT PROTEIN; HISTIDINE; MALEIMIDE; PROTEIN; SILICON; THIOL DERIVATIVE;

ARTICLE; CHEMOLUMINESCENCE; DNA SEQUENCE; ESCHERICHIA COLI; FLUORESCENCE; FUNCTIONAL PROTEOMICS; HIGH THROUGHPUT SCREENING; MASS SPECTROMETRY; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN MICROARRAY; PROTEIN PURIFICATION; PROTEOMICS; TECHNOLOGY;

CYSTEINE; DNA PRIMERS; GREEN FLUORESCENT PROTEINS; OLIGOPEPTIDES; PROTEIN ARRAY ANALYSIS; PROTEOMICS; RECOMBINANT FUSION PROTEINS; STATHMIN;

ESCHERICHIA COLI;

EID: 33748298796     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr0504889     Document Type: Article

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