Capillary electrophoresis with laser-induced fluorescence detection as a tool for enzyme characterization and inhibitor screening

Analytical Sciences

Volumn 24, Issue 3, 2008, Pages 333-337

  Liu, Dan Ning ^a   Li, Li ^b   Lu, Wei Ping ^b   Liu, Yao Qing D ^b   Wehmeyer, Ken R ^b   Bao, James J ^a  

^a TIANJIN UNIVERSITY   (China)

^b PROCTER AND GAMBLE PHARMACEUTICALS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAPILLARY ELECTROPHORESIS; CONTROLLED DRUG DELIVERY; FLUORESCENCE;

CROSS REACTIVITY; ENZYME CHARACTERIZATION; FUNCTION OF TIME; HIGH-THROUGHPUT SCREENING; INHIBITOR SCREENINGS; LASER INDUCED FLUORESCENCE DETECTION; REACTION CONDITIONS; SIGNAL PEPTIDASE;

ENZYME ACTIVITY;

DIMETHYL SULFOXIDE; ENZYME INHIBITOR; MEMBRANE PROTEIN; PEPTIDE FRAGMENT; SERINE PROTEINASE; TYPE I SIGNAL PEPTIDASE; UREA;

ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; DRUG SCREENING; ENZYME ACTIVATION; FLUORESCENCE; INSTRUMENTATION; LASER; LIGHT; METHODOLOGY; PH; RADIATION EXPOSURE; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY; STRUCTURE ACTIVITY RELATION; TIME;

DIMETHYL SULFOXIDE; DRUG EVALUATION, PRECLINICAL; ELECTROPHORESIS, CAPILLARY; ENZYME ACTIVATION; ENZYME INHIBITORS; FLUORESCENCE; HYDROGEN-ION CONCENTRATION; LASERS; LIGHT; MEMBRANE PROTEINS; PEPTIDE FRAGMENTS; SENSITIVITY AND SPECIFICITY; SERINE ENDOPEPTIDASES; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; TIME FACTORS; UREA;

EID: 48449098296     PISSN: 09106340     EISSN: 13482246     Source Type: Journal    
DOI: 10.2116/analsci.24.333     Document Type: Article

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