Chemically well-defined self-assembled monolayers for cell culture: Toward mimicking the natural ECM

Soft Matter

Volumn 7, Issue 20, 2011, Pages 9561-9571

  Hudalla, Gregory A ^a,b   Murphy, William L ^a  

^a University of Wisconsin Health   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MACROMOLECULE; CELL ATTACHMENTS; CELL BEHAVIORS; CELL BIOLOGY; CELL TYPES; CHEMICAL SPECIFICITY; COMPLEX FORMATIONS; COVALENT IMMOBILIZATION; CULTURE SUBSTRATE; EXPERIMENTAL DESIGN AND ANALYSIS; EXTRACELLULAR MATRICES; FOCAL ADHESIONS; GROWTH FACTOR; NONSPECIFIC ADSORPTION; SAMS; SIGNALING MOLECULES;

ADHESION; ADSORPTION; BIOMOLECULES; CELL ADHESION; CELLS; CHEMICAL ANALYSIS; SELF ASSEMBLED MONOLAYERS; SUBSTRATES; TISSUE;

CELL CULTURE;

EID: 80053617363     PISSN: 1744683X     EISSN: 17446848     Source Type: Journal    
DOI: 10.1039/c1sm05596h     Document Type: Review

References (113)

1. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter, in Molecular Biology of the Cell, ed., G. Science,. 4th edn, 2002, pp 1090-1113
2. M. van der Rest R. Garrone Collagen family of proteins Faseb J 1991 5 2814 23 (Pubitemid 21892853)
3. C. Gentili R. Cancedda Cartilage and bone extracellular matrix Curr. Pharm. Des. 2009 15 1334 48
4. J. Rosenbloom W. R. Abrams R. Mecham Extracellular matrix 4: the elastic fiber Faseb J 1993 7 1208 18 (Pubitemid 23314355)
5. E. Ruoslahti Brain extracellular matrix Glycobiology 1996 6 489 92 (Pubitemid 26298189)
6. K. Y. Tsang M. C. Cheung D. Chan K. S. Cheah The developmental roles of the extracellular matrix: beyond structure to regulation Cell Tissue Res. 2010 339 93 110
7. F. Guilak D. M. Cohen B. T. Estes J. M. Gimble W. Liedtke C. S. Chen Control of stem cell fate by physical interactions with the extracellular matrix Cell Stem Cell 2009 5 17 26
8. G. C. Reilly A. J. Engler Intrinsic extracellular matrix properties regulate stem cell differentiation J. Biomech. 2010 43 55 62
9. A. Kadouri D. Sher N. G. Maroudas Polystyrene substratum for bulk culture of anchorage dependent cells Cytotechnology 1988 301 307
10. L. Vroman Effect of absorbed proteins on the wettability of hydrophilic and hydrophobic solids Nature 1962 196 476 7
11. E. Cukierman R. Pankov K. M. Yamada Cell interactions with three-dimensional matrices Curr. Opin. Cell Biol. 2002 14 633 9 10.1016/S0955-0674(02)00364-2 (Pubitemid 35247748)
12. L. G. Griffith M. A. Swartz Capturing complex 3D tissue physiology in vitro Nat. Rev. Mol. Cell Biol. 2006 7 211 24 10.1038/nrm1858
13. S. Khetan J. Burdick Cellular encapsulation in 3D hydrogels for tissue engineering J Vis Exp 2009
14. J. P. Jung J. Z. Gasiorowski J. H. Collier Fibrillar peptide gels in biotechnology and biomedicine Biopolymers 2010 94 49 59
15. G. A. Hudalla W. L. Murphy Biomaterials that regulate growth factor activity via bioinspired interactions Adv. Funct. Mater. 2011 21 1754 1768
16. E. H. Nguyen M. P. Schwartz W. L. Murphy Biomimetic approaches to control soluble concentration gradients in biomaterials Macromol. Biosci. 2011 11 483 92 10.1002/mabi.201000448
17. K. Y. Lee D. J. Mooney Hydrogels for tissue engineering Chem. Rev. 2001 101 1869 79 10.1021/cr000108x (Pubitemid 35377789)
18. M. P. Lutolf J. A. Hubbell Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering Nat. Biotechnol. 2005 23 47 55 10.1038/nbt1055 (Pubitemid 41724624)
19. S. J. Singer G. L. Nicolson The fluid mosaic model of the structure of cell membranes Science 1972 175 720 31
20. G. D. Pins D. L. Christiansen R. Patel F. H. Silver Self-assembly of collagen fibers. Influence of fibrillar alignment and decorin on mechanical properties Biophys. J. 1997 73 2164 72 (Pubitemid 27425747)
21. K. E. Kadler A. Hill E. G. Canty-Laird Collagen fibrillogenesis: fibronectin, integrins, and minor collagens as organizers and nucleators Curr. Opin. Cell Biol. 2008 20 495 501
22. R. G. Nuzzo D. L. Allara Adsorption of bifunctional organic disulfides on gold surfaces J. Am. Chem. Soc. 1983 105 4481 4483
23. C. D. Bain B. Troughton Y. T. Tao J. Evall G. M. Whitesides R. G. Nuzzo Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold J. Am. Chem. Soc. 1989 111 321 335
24. L. Strong G. M. Whitesides Structures of self-assembled monolayer films of organosulfur compounds adsorbed on gold single crystals: electron diffraction studies Langmuir 1988 4 546 558
25. T. Wink S. J. van Zuilen A. Bult W. P. van Bennkom Self-assembled monolayers for biosensors Analyst 1997 122 43R 50R (Pubitemid 27189831)
26. M. Kind C. Woll Organic surfaces exposed by self-assembled organothiol monolayers: Preparation, characterization, and application Prog. Surf. Sci. 2009 84 230 278
27. M. Mrksich Mass spectrometry of self-assembled monolayers: a new tool for molecular surface science ACS Nano 2008 2 7 18 (Pubitemid 351585759)
28. J. Li P. S. Thiara M. Mrksich Rapid Evalulation and Screeing of Interfacial Reactions on Self-Assembled Monolayers Langmuir 2007 23 11826 11835 (Pubitemid 350133033)
29. N. K. Chaki K. Vijayamohanan Self-assembled monolayers as a tunable platform for biosensor applications Biosens. Bioelectron. 2002 17 1 12 (Pubitemid 34003604)
30. D. Mandler I. Turyan Applications of self-assembled monolayers in electroanalytical chemistry Electroanalysis 2005 8 207 213
31. C. D. Bain G. M. Whitesides Formation of two-component surfaces by the spontaneous assembly of monolayers on gold from solutions containing mixtures of organic thiols J. Am. Chem. Soc. 1988 110 6560 6561
32. M. A. Chesters G. A. Somorjai The chemisorption of oxygen, water and selected hydrocarbons on the (111) and stepped gold surfaces Surf. Sci. 1975 52 21 28
33. D. Witt R. Klajn P. Barski B. A. Grzybowski Applications, properties and synthesis of omega-functionalized n-alkanethiols and disulfides-the building blocks of self-assembled monolayers Curr. Org. Chem. 2004 8 1763 1797 (Pubitemid 39562083)
34. Y. Arima H. Iwata Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers J. Mater. Chem. 2007 17 4079 4087 (Pubitemid 47482965)
35. C. D. Tidwell S. I. Ertel B. D. Ratner B. J. Tarasevich S. Atre D. L. Allara Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkanethiolates on gold Langmuir 1997 13 3404 3413 (Pubitemid 127604444)
36. H. Wang Y. He B. D. Ratner S. Jiang Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates Journal of biomedical materials research 2006 77 672 8 (Pubitemid 47240770)
37. L. Liu S. Chen C. M. Giachelli B. D. Ratner S. Jiang Controlling osteopontin orientation on surfaces to modulate endothelial cell adhesion Journal of biomedical materials research 2005 74 23 31 (Pubitemid 41099247)
38. C. C. Barrias M. C. Martins G. Almeida-Porada M. A. Barbosa P. L. Granja The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers Biomaterials 2009 30 307 16
39. W. H. Chuang J. C. Lin Surface characterization and platelet adhesion studies for the mixed self-assembled monolayers with amine and carboxylic acid terminated functionalities Journal of biomedical materials research 2007 82 820 30
40. M. Y. Tsai J. C. Lin Surface characterization and platelet adhesion studies of self-assembled monolayer with phosphonate ester and phosphonic acid functionalities J. Biomed. Mater. Res. 2001 55 554 65
41. S. N. Rodrigues I. C. Goncalves M. C. Martins M. A. Barbosa B. D. Ratner Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers Biomaterials 2006 27 5357 67 (Pubitemid 44093714)
42. Y. C. Chung Y. H. Chiu Y. W. Wu Y. T. Tao Self-assembled biomimetic monolayers using phospholipid-containing disulfides Biomaterials 2005 26 2313 24
43. E. Cooper L. Parker C. A. Scotchford S. Downes G. J. Leggett T. L. Parker The effect of alkyl chain length and terminal group chemistry on the attachment and growth of murine 3T3 fibroblasts and primary human osteoblasts on self-assembled monolayers of alkanethiols on gold J. Mater. Chem. 2000 10 133 139
44. B. G. Keselowsky D. M. Collard A. J. Garcia Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion J. Biomed. Mater. Res. 2003 66 247 59 (Pubitemid 37517224)
45. C. A. Scotchford C. P. Gilmore E. Cooper G. J. Leggett S. Downes Protein adsorption and human osteoblast-like cell attachment and growth on alkylthiol on gold self-assembled monolayers J. Biomed. Mater. Res. 2002 59 84 99
46. V. A. Tegoulia W. S. Rao A. T. Kalambur J. R. Rabolt S. L. Cooper Surface properties, fibrinogen adsorption, and cellular interactions of a novel phosphorylcholine-containing self-assembled monolayer on gold Langmuir 2001 17 4396 4404 (Pubitemid 35330312)
47. M. Franco P. F. Nealey S. Campbell A. I. Teixeira C. J. Murphy Adhesion and proliferation of corneal epithelial cells on self-assembled monolayers J. Biomed. Mater. Res. 2000 52 261 9 (Pubitemid 30646797)
48. V. A. Tegoulia S. L. Cooper Leukocyte adhesion on model surfaces under flow: effects of surface chemistry, protein adsorption, and shear rate J. Biomed. Mater. Res. 2000 50 291 301 (Pubitemid 30180721)
49. C. Sperling R. B. Schweiss U. Streller C. Werner In vitro hemocompatibility of self-assembled monolayers displaying various functional groups Biomaterials 2005 26 6547 57 (Pubitemid 41024930)
50. S. Inoue M. Imamura A. Umezawa Y. Tabata Attachment, proliferation and adipogenic differentiation of adipo-stromal cells on self-assembled monolayers of different chemical compositions J. Biomater. Sci., Polym. Ed. 2008 19 893 914
51. E. Cooper R. Wiggs D. A. Hutt L. Parker G. J. Leggett T. L. Parker Rates of attachment of fibroblasts to self-assembled monolayers formed by the adsorption of alkylthiols onto gold surfaces J. Mater. Chem. 1997 7 435 441 (Pubitemid 127728791)
52. K. B. McClary T. Ugarova D. W. Grainger Modulating fibroblast adhesion, spreading, and proliferation using self-assembled monolayer films of alkylthiolates on gold J. Biomed. Mater. Res. 2000 50 428 39 (Pubitemid 30180736)
53. C. A. Scotchford E. Cooper G. J. Leggett S. Downes Growth of human osteoblast-like cells on alkanethiol on gold self-assembled monolayers: the effect of surface chemistry J. Biomed. Mater. Res. 1998 41 431 42 (Pubitemid 28300108)
54. D. B. Haddow R. M. France R. D. Short S. MacNeil R. A. Dawson G. J. Leggett E. Cooper Comparison of proliferation and growth of human keratinocytes on plasma copolymers of acrylic acid/1,7-octadiene and self-assembled monolayers J. Biomed. Mater. Res. 1999 47 379 87
55. M. A. Lan C. A. Gersbach K. E. Michael B. G. Keselowsky A. J. Garcia Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries Biomaterials 2005 26 4523 31 (Pubitemid 40269268)
56. E. V. Romanova S. P. Oxley S. S. Rubakhin P. W. Bohn J. V. Sweedler Self-assembled monolayers of alkanethiols on gold modulate electrophysiological parameters and cellular morphology of cultured neurons Biomaterials 2006 27 1665 9 (Pubitemid 41739592)
57. J. E. Phillips T. A. Petrie F. P. Creighton A. J. Garcia Human mesenchymal stem cell differentiation on self-assembled monolayers presenting different surface chemistries Acta Biomater. 2010 6 12 20
58. R. McBeath D. M. Pirone C. M. Nelson K. Bhadriraju C. S. Chen Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment Dev. Cell 2004 6 483 95 (Pubitemid 38456178)
59. K. A. Kilian B. Bugarija B. T. Lahn M. Mrksich Geometric cues for directing the differentiation of mesenchymal stem cells Proc. Natl. Acad. Sci. U. S. A. 2010 107 4872 7
60. B. G. Keselowsky D. M. Collard A. J. Garcia Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation Proc. Natl. Acad. Sci. U. S. A. 2005 102 5953 7 (Pubitemid 40594236)
61. R. Nakaoka Y. Yamakoshi K. Isama T. Tsuchiya Effects of surface chemistry prepared by self-assembled monolayers on osteoblast behavior Journal of biomedical materials research 2010
62. B. Valamehr S. J. Jonas J. Polleux R. Qiao S. Guo E. H. Gschweng B. Stiles K. Kam T. J. Luo O. N. Witte X. Liu B. Dunn H. Wu Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies Proc. Natl. Acad. Sci. U. S. A. 2008 105 14459 64
63. Y. Naka A. Eda H. Takei N. Shimizu Neurite outgrowths of neurons on patterned self-assembled monolayers Journal of bioscience and bioengineering 2002 94 434 9 (Pubitemid 36246185)
64. Poly(ethylene glycol): Chemistry and Biological Applications. American Chemical Society: 1997
65. C. Palegrosdemange E. S. Simon K. L. Prime G. M. Whitesides Formation of Self-Assembled Monolayers by Chemisorption of Derivatives of Oligo(Ethylene Glycol) of Structure Hs(Ch2)11(Och2ch2)Meta-Oh on Gold J. Am. Chem. Soc. 1991 113 12 20
66. C. S. Chen M. Mrksich S. Huang G. M. Whitesides D. E. Ingber Geometric control of cell life and death Science 1997 276 1425 8 (Pubitemid 27235413)
67. C. Roberts C. S. Chen M. Mrksich V. Martichonok D. E. Ingber G. M. Whitesides Using mixed self-assembled monolayers presenting RGD and (EG)(3)OH groups to characterize long-term attachment of bovine capillary endothelial cells to surfaces J. Am. Chem. Soc. 1998 120 6548 6555 (Pubitemid 28345220)
68. B. T. Houseman M. Mrksich The microenvironment of immobilized Arg-Gly-Asp peptides is an important determinant of cell adhesion Biomaterials 2001 22 943 955 (Pubitemid 32201445)
69. V. C. Hirschfeld-Warneken M. Arnold A. Cavalcanti-Adam M. Lopez-Garcia H. Kessler J. P. Spatz Cell adhesion and polarisation on molecularly defined spacing gradient surfaces of cyclic RGDfK peptide patches Eur. J. Cell Biol. 2008 87 743 750
70. E. A. Cavalcanti-Adam A. Micoulet J. Blummel J. Auernheimer H. Kessler J. P. Spatz Lateral spacing of integrin ligands influences cell spreading and focal adhesion assembly Eur. J. Cell Biol. 2006 85 219 224
71. E. A. Cavalcanti-Adam T. Volberg A. Micoulet H. Kessler B. Geiger J. P. Spatz Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands Biophys. J. 2007 92 2964 2974 (Pubitemid 46557869)
72. R. Derda L. Y. Li B. P. Orner R. L. Lewis J. A. Thomson L. L. Kiessling Defined substrates for human embryonic stem cell growth identified from surface arrays ACS Chem. Biol. 2007 2 347 355 (Pubitemid 350001583)
73. R. Derda S. Musah B. P. Orner J. R. Klim L. Li L. L. Kiessling High-throughput discovery of synthetic surfaces that support proliferation of pluripotent cells J. Am. Chem. Soc. 2010 132 1289 95
74. J. Lahiri L. Isaacs J. Tien G. M. Whitesides A strategy for the generation of surfaces presenting ligands for studies of binding based on an active ester as a common reactive intermediate: a surface plasmon resonance study Anal. Chem. 1999 71 777 90
75. B. T. Houseman E. S. Gawalt M. Mrksich Maleimide-Functionalized Self-Assembled Monolayers for the Preparation of Peptide and Carbohydrate Biochips Langmuir 2003 19 1522 1531
76. C. D. Hahn C. Leitner T. Weinbrenner R. Schlapak A. Tinazli R. Tampe B. Lackner C. Steindl P. Hinterdorfer H. J. Gruber M. Holzl Self-assembled monolayers with latent aldehydes for protein immobilization Bioconjugate Chem. 2007 18 247 53 10.1021/bc060292e (Pubitemid 46146016)
77. K. Tashiro G. C. Sephel B. Weeks M. Sasaki G. R. Martin H. K. Kleinman Y. Yamada A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth The Journal of biological chemistry 1989 264 16174 82 (Pubitemid 19238381)
78. K. Jans B. Van Meerbergen G. Reekmans K. Bonroy W. Annaert G. Maes Y. Engelborghs G. Borghs C. Bartic Chemical and biological characterization of thiol SAMs for neuronal cell attachment Langmuir 2009 25 4564 70
79. R. Knerr B. Weiser S. Drotleff C. Steinem A. Gopferich Measuring cell adhesion on RGD-modified, self-assembled PEG monolayers using the quartz crystal microbalance technique Macromol. Biosci. 2006 6 827 38 (Pubitemid 44831728)
80. S. H. Shabbir J. L. Eisenberg M. Mrksich An inhibitor of a cell adhesion receptor stimulates cell migration Angew. Chem., Int. Ed. 2010 49 7706 9 10.1002/anie.201002699
81. J. T. Smith D. H. Kim W. M. Reichert Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors Comb. Chem. High Throughput Screening 2009 12 598 603
82. L. Liu B. D. Ratner E. H. Sage S. Jiang Endothelial cell migration on surface-density gradients of fibronectin, VEGF, or both proteins Langmuir 2007 23 11168 73 (Pubitemid 350069342)
83. R. Goncalves, M. C. Martins, M. J. Oliveira, G. Almeida-Porada and M. A. Barbosa, Bioactivity of immobilized EGF on self-assembled monolayers: Optimization of the immobilization process. Journal of Biomedical Materials Research
84. D. Peelen V. Kodoyianni J. Lee T. Zheng M. R. Shortreed L. M. Smith Specific capture of mammalian cells by cell surface receptor binding to ligand immobilized on gold thin films J. Proteome Res. 2006 5 1580 5 (Pubitemid 44036132)
85. Y. Feng M. Mrksich The synergy peptide PHSRN and the adhesion peptide RGD mediate cell adhesion through a common mechanism Biochemistry 2004 43 15811 21 (Pubitemid 39665080)
86. M. Kato M. Mrksich Using model substrates to study the dependence of focal adhesion formation on the affinity of integrin-ligand complexes Biochemistry 2004 43 2699 707 (Pubitemid 38327801)
87. C. D. Hodneland Y. S. Lee D. H. Min M. Mrksich Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands Proc. Natl. Acad. Sci. U. S. A. 2002 99 5048 52 (Pubitemid 34411501)
88. W. L. Murphy K. O. Mercurius S. Koide M. Mrksich Substrates for cell adhesion prepared via active site-directed immobilization of a protein domain Langmuir 2004 20 1026 30
89. J. L. Eisenberg J. L. Piper M. Mrksich Using self-assembled monolayers to model cell adhesion to the 9th and 10th type III domains of fibronectin Langmuir 2009 25 13942 13951
90. G. A. Hudalla W. L. Murphy Using "click" chemistry to prepare SAM substrates to study stem cell adhesion Langmuir 2009 25 5737 46 10.1021/la804077t
91. W. S. Dillmore M. N. Yousaf M. Mrksich A photochemical method for patterning the immobilization of ligands and cells to self-assembled monolayers Langmuir 2004 20 7223 31
92. M. N. Yousaf B. T. Houseman M. Mrksich Turning On Cell Migration with Electroactive Substrates Angew. Chem., Int. Ed. 2001 40 1093 1096
93. R. T. Petty H. W. Li J. H. Maduram R. Ismagilov M. Mrksich Attachment of cells to islands presenting gradients of adhesion ligands J. Am. Chem. Soc. 2007 129 8966 7 (Pubitemid 47171837)
94. N. P. Westcott A. Pulsipher B. M. Lamb M. N. Yousaf Expedient generation of patterned surface aldehydes by microfluidic oxidation for chemoselective immobilization of ligands and cells Langmuir 2008 24 9237 40
95. J. T. Koepsel W. L. Murphy Patterning discrete stem cell culture environments via localized self-assembled monolayer replacement Langmuir 2009 25 12825 34
96. S. M. Albelda C. A. Buck Integrins and other cell adhesion molecules Faseb J 1990 4 2868 80 (Pubitemid 120012533)
97. G. A. Hudalla W. L. Murphy Immobilization of peptides with distinct biological activities onto stem cell culture substrates using orthogonal chemistries Langmuir 2010 26 6449 56 10.1021/la1008208
98. R. M. Goncalves M. C. Martins G. Almeida-Porada M. A. Barbosa Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers Biomaterials 2009 30 6879 87
99. S. Chiba Notch signaling in stem cell systems Stem Cells 2006 24 2437 47 (Pubitemid 44674304)
100. R. Gentz C. H. Chen C. A. Rosen Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis Proc. Natl. Acad. Sci. U. S. A. 1989 86 821 4 (Pubitemid 19056248)
101. T. Nakaji-Hirabayashi K. Kato Y. Arima H. Iwata Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells Biomaterials 2007 28 3517 29 (Pubitemid 46817654)
102. K. Kato H. Sato H. Iwata Immobilization of histidine-tagged recombinant proteins onto micropatterned surfaces for cell-based functional assays Langmuir 2005 21 7071 5 (Pubitemid 41156716)
103. J. Sanchez-Cortes K. Bahr M. Mrksich Cell adhesion to unnatural ligands mediated by a bifunctional protein J. Am. Chem. Soc. 2010 132 9733 7 10.1021/ja1016188
104. K. Kottkemarchant J. M. Anderson Y. Umemura R. E. Marchant Effect of albumin coating on the in vitro blood compatibility of dacron arterial prostheses Biomaterials 1989 25 5681 5703
105. M. S. Munro A. J. Quattrone S. R. Ellsworth P. Kulkarni R. C. Eberhart Alkyl substituted polymers with enhanced albumin affinity Transactions-American Society for Artificial Internal Organs 1981 27 499 503
106. I. C. Goncalves M. C. Martins M. A. Barbosa E. Naeemi B. D. Ratner Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands Journal of biomedical materials research 2009 89 642 53
107. M. E. Levenstein W. T. Berggren J. E. Lee K. R. Conard R. A. Llanas R. J. Wagner L. M. Smith J. A. Thomson Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation Stem Cells 2008 26 3099 107
108. K. E. Healy A. Rezania R. A. Stile Designing biomaterials to direct biological responses Ann. N. Y. Acad. Sci. 1999 875 24 35 (Pubitemid 29349059)
109. T. Nie R. E. AkinsJr. K. L. Kiick Production of heparin-containing hydrogels for modulating cell responses Acta Biomater. 2009 5 865 75 10.1016/j.actbio.2008.12.004
110. D. S. Benoit K. S. Anseth Heparin functionalized PEG gels that modulate protein adsorption for hMSC adhesion and differentiation Acta Biomater. 2005 1 461 70 10.1016/j.actbio.2005.03.002 (Pubitemid 43348782)
111. R. Haas L. A. Culp Properties and fate of plasma fibronectin bound to the tissue culture substratum J. Cell. Physiol. 1982 113 289 97 10.1002/jcp.1041130217 (Pubitemid 13231429)
112. Z. Avnur B. Geiger The removal of extracellular fibronectin from areas of cell-substrate contact Cell 1981 25 121 32 10.1016/0092-8674(81)90236-1 (Pubitemid 11087440)
113. F. Grinnell Focal adhesion sites and the removal of substratum-bound fibronectin J. Cell Biol. 1986 103 2697 706 (Pubitemid 17008015)