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Volumn 20, Issue 4, 2004, Pages 1026-1030

Substrates for Cell Adhesion Prepared via Active Site-Directed Immobilization of a Protein Domain

Author keywords

[No Author keywords available]

Indexed keywords

CELL ADHESION; LIGANDS;

EID: 1442283948     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la035733m     Document Type: Article
Times cited : (42)

References (28)
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    • note
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    • [Albino] Swiss 3T3 fibrofalasts and IMR-90 human lung fibro-blasts (obtained from ATCC, Manassas, VA) were cultured in DMEM + 10% fetal bovine serum (FBS) + antibiotics. For all cell adhesion assays on SAMs, cells were harvested by trypsin/EDTA treatment, collected, and then washed and resuspended in their respective growth medium. Suspensions of 150 000 cells/mL were added to each substrate and incubated at 37°C for 1 h. Substrates were then washed 3′ with PBS, fixed in 3.7% paraformaldehyde at 37°C for 20 min, mounted with 1:1 glycerol/PBS, and visualized with a Zeiss Axiovert 135 microscope.
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    • note
    • The number of attached cells was quantified manually by counting the number of cells in four separate fields of view per sample, and four samples were analyzed per condition. Student's t-tests were used to identify statistically significant differences in attached cell number.
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