-
1
-
-
0035342522
-
The microenvironment of immobilized Arg-Gly-Asp peptides is an important determinant of cell adhesion
-
Houseman, B. T.; Mrksich, M. The microenvironment of immobilized Arg-Gly-Asp peptides is an important determinant of cell adhesion. Biomaterials 2001, 22, 943-955.
-
(2001)
Biomaterials
, vol.22
, pp. 943-955
-
-
Houseman, B.T.1
Mrksich, M.2
-
2
-
-
0025881229
-
An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation
-
Massia, S. P.; Hubbell, J. A. An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation. J. Cell Biol. 1991, 114, 1089-1100.
-
(1991)
J. Cell Biol.
, vol.114
, pp. 1089-1100
-
-
Massia, S.P.1
Hubbell, J.A.2
-
3
-
-
0035857564
-
Turning on cell migration with electroactive substrates
-
Yousaf, M. N.; Houseman, B. T.; Mrksich, M. Turning on cell migration with electroactive substrates. Angew. Chem., Int. Ed. 2001, 40, 1093-1096.
-
(2001)
Angew. Chem., Int. Ed.
, vol.40
, pp. 1093-1096
-
-
Yousaf, M.N.1
Houseman, B.T.2
Mrksich, M.3
-
4
-
-
0036185659
-
Alginate type and RGD density control myoblast phenotype
-
Rowley, J. A.; Mooney, D. J. Alginate type and RGD density control myoblast phenotype. J. Biomed. Mater. Res. 2002, 60, 217-223.
-
(2002)
J. Biomed. Mater. Res.
, vol.60
, pp. 217-223
-
-
Rowley, J.A.1
Mooney, D.J.2
-
5
-
-
0035800893
-
Electroactive monolayer substrates that selectively release adherent cells
-
Yeo, W. S.; Hodneland, C. D.; Mrksich, M. Electroactive monolayer substrates that selectively release adherent cells. ChemBioChem 2001, 2, 590-593.
-
(2001)
ChemBioChem
, vol.2
, pp. 590-593
-
-
Yeo, W.S.1
Hodneland, C.D.2
Mrksich, M.3
-
6
-
-
0025300277
-
Covalent surface immobilization of Arg-Gly-Asp- and Tyr-Ile-Gly-Ser-Arg-containing peptides to obtain well-defined cell-adhesive substrates
-
Massia, S. P.; Hubbell, J. A. Covalent surface immobilization of Arg-Gly-Asp- and Tyr-Ile-Gly-Ser-Arg-containing peptides to obtain well-defined cell-adhesive substrates. Anal. Biochem. 1990, 187, 292-301.
-
(1990)
Anal. Biochem.
, vol.187
, pp. 292-301
-
-
Massia, S.P.1
Hubbell, J.A.2
-
7
-
-
0001107373
-
Structural changes in proteins adsorbed on polymer surfaces
-
Soderquist, M. E.; Walton, A. G. Structural changes in proteins adsorbed on polymer surfaces. J. Colloid Interface Sci. 1980, 75, 386-397.
-
(1980)
J. Colloid Interface Sci.
, vol.75
, pp. 386-397
-
-
Soderquist, M.E.1
Walton, A.G.2
-
8
-
-
0029251458
-
Structures and stabilities of adsorbed proteins
-
Haynes, C.; Norde, W. Structures and stabilities of adsorbed proteins. J. Colloid Interface Sci. 1995, 169, 313-328.
-
(1995)
J. Colloid Interface Sci.
, vol.169
, pp. 313-328
-
-
Haynes, C.1
Norde, W.2
-
9
-
-
0344339177
-
Unfolding and intermolecular association in globular proteins adsorbed at interfaces
-
Green, R. J.; Hopkinson, I.; Jones, R. A. L. Unfolding and intermolecular association in globular proteins adsorbed at interfaces. Langmuir 1999, 15, 5102-5110.
-
(1999)
Langmuir
, vol.15
, pp. 5102-5110
-
-
Green, R.J.1
Hopkinson, I.2
Jones, R.A.L.3
-
10
-
-
0028790068
-
Changes in organization and composition of the extracellular matrix underlying cultured endothelial cells exposed to laminar steady shear stress
-
Thoumine, O.; Nerem, R. M.; Girard, P. R. Changes in organization and composition of the extracellular matrix underlying cultured endothelial cells exposed to laminar steady shear stress. Lab. Invest. 1995, 73, 565-576.
-
(1995)
Lab. Invest.
, vol.73
, pp. 565-576
-
-
Thoumine, O.1
Nerem, R.M.2
Girard, P.R.3
-
11
-
-
0032728826
-
Substrate-adsorbed collagen and cell secreted fibronectin concertedly induce cell migration on poly(lactide-glycolide) substrates
-
Tjia, J. S.; Aneskievich, B. J.; Moghe, P. V. Substrate-adsorbed collagen and cell secreted fibronectin concertedly induce cell migration on poly(lactide-glycolide) substrates. Biomaterials 1999, 20, 2223-2233.
-
(1999)
Biomaterials
, vol.20
, pp. 2223-2233
-
-
Tjia, J.S.1
Aneskievich, B.J.2
Moghe, P.V.3
-
12
-
-
0343457899
-
Adsorption and relaxation kinetics of albumin and fibrinogen on hydrophobic surfaces: Single-species and competitive behavior
-
Wertz, C. F.; Santore, M. M. Adsorption and relaxation kinetics of albumin and fibrinogen on hydrophobic surfaces: Single-species and competitive behavior. Langmuir 1999, 15, 8884-8894.
-
(1999)
Langmuir
, vol.15
, pp. 8884-8894
-
-
Wertz, C.F.1
Santore, M.M.2
-
13
-
-
0037117516
-
Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands
-
Hodneland, C. D.; Lee, Y. S.; Min, D. H.; Mrksich, M. Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 5048-5052.
-
(2002)
Proc. Natl. Acad. Sci. U.S.A.
, vol.99
, pp. 5048-5052
-
-
Hodneland, C.D.1
Lee, Y.S.2
Min, D.H.3
Mrksich, M.4
-
14
-
-
0028812770
-
Phosphonate analogues of triacylglycerols are potent inhibitors of lipase
-
Mannesse, M. L.; Boots, J. W.; Dijkman, R.; Slotboom, A. J.; van der Hijden, H. T.; Egmond, M. R.; Verheij, H. M.; de Haas, G. H. Phosphonate analogues of triacylglycerols are potent inhibitors of lipase. Biochim. Biophys. Acta 1995, 1259, 56-64.
-
(1995)
Biochim. Biophys. Acta
, vol.1259
, pp. 56-64
-
-
Mannesse, M.L.1
Boots, J.W.2
Dijkman, R.3
Slotboom, A.J.4
Van der Hijden, H.T.5
Egmond, M.R.6
Verheij, H.M.7
De Haas, G.H.8
-
15
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0037424598
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Engineered fibronectin type III domain with a RGDWXE sequence binds with enhanced affinity and specificity to human alphavbeta3 integrin
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Richards, J.; Miller, M.; Abend, J.; Koide, A.; Koide, S.; Dewhurst, S. Engineered fibronectin type III domain with a RGDWXE sequence binds with enhanced affinity and specificity to human alphavbeta3 integrin. J. Mol. Biol. 2003, 326, 1475-1488.
-
(2003)
J. Mol. Biol.
, vol.326
, pp. 1475-1488
-
-
Richards, J.1
Miller, M.2
Abend, J.3
Koide, A.4
Koide, S.5
Dewhurst, S.6
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16
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1442301490
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PCR primers: 5′-CTAGGATCCATGCAGGTTTCTGATGTTCCG-3′ and 5′-TCGATCCTCGAGTTACTATTTACCTTTTTTACC-3′, from Genosys
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PCR primers: 5′-CTAGGATCCATGCAGGTTTCTGATGTTCCG-3′ and 5′-TCGATCCTCGAGTTACTATTTACCTTTTTTACC-3′, from Genosys
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17
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1442325998
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note
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Cloning was via the BamH1 and Xho1 restriction sites, at the 3′ end (C-terminus) of the cutinase gene; restriction enzymes were from New England Biolabs.
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18
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1442350409
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note
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600 = 0.5 before induction with 0.5 mM isopropyl β-D-thiogalactopyranoside at 25°C for 18 h. Cells were collected by centrifugation at 5000g for 15 min and resuspended in 20 mL of PBS. Expressed protein was then liberated via sonication, and the resulting solution was clarified by centrifugation.
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19
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1442276956
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note
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All SPR experiments were performed with a Biacore 1000 instrument; all experiments used a flow rate of 1 μL/mm at 25°C in PBS (pH = 7.4).
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20
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0032522286
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Effect of surface wettability on the adsorption of proteins and detergents
-
Sigal, G. B, M. M.; Whitesides, G. M. Effect of surface wettability on the adsorption of proteins and detergents. J. Am. Chem. Soc. 1998, 120, 3464-3473.
-
(1998)
J. Am. Chem. Soc.
, vol.120
, pp. 3464-3473
-
-
Sigal, G.B.1
M., M.2
Whitesides, G.M.3
-
21
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1442276952
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note
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[Albino] Swiss 3T3 fibrofalasts and IMR-90 human lung fibro-blasts (obtained from ATCC, Manassas, VA) were cultured in DMEM + 10% fetal bovine serum (FBS) + antibiotics. For all cell adhesion assays on SAMs, cells were harvested by trypsin/EDTA treatment, collected, and then washed and resuspended in their respective growth medium. Suspensions of 150 000 cells/mL were added to each substrate and incubated at 37°C for 1 h. Substrates were then washed 3′ with PBS, fixed in 3.7% paraformaldehyde at 37°C for 20 min, mounted with 1:1 glycerol/PBS, and visualized with a Zeiss Axiovert 135 microscope.
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22
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0035954424
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Chinese hamster ovary (CHO)-B2, -B2/v7, and -B2/α27 cells (a generous gift from Dr. Erkki Ruoslahti, Burnham Institute, La Jolla, CA) were cultured in α-MEM + 10% FBS + antibiotics. G418 (400 μg/ml,) was added to the medium of the B2/v7 and B2/α27 cells. CHO cells expressing GFP-labeled paxillin (a generous gift from Dr. Alan F. Horwitz, University of Virginia) were cultured as previously described: Laukaitis, C. M.; Webb, D. J.; Donais, K.; Horwitz, A. F. J. Cell. Biol. 2001, 153, 1427-1440.
-
(2001)
J. Cell. Biol.
, vol.153
, pp. 1427-1440
-
-
Laukaitis, C.M.1
Webb, D.J.2
Donais, K.3
Horwitz, A.F.4
-
23
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0024836021
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Isolation and characterization of Chinese hamster ovary cell variants deficient in the expression of fibronectin receptor
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Schreiner, C. L.; Bauer, J. S.; Danilov, Y. N.; Hussein, S.; Sczekan, M. M.; Juliano, R. L. Isolation and characterization of Chinese hamster ovary cell variants deficient in the expression of fibronectin receptor. J. Cell. Biol. 1989, 109, 3157-3167.
-
(1989)
J. Cell. Biol.
, vol.109
, pp. 3157-3167
-
-
Schreiner, C.L.1
Bauer, J.S.2
Danilov, Y.N.3
Hussein, S.4
Sczekan, M.M.5
Juliano, R.L.6
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24
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0027175838
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The alpha v beta 1 integrin functions as a fibronectin receptor but does not support fibronectin matrix assembly and cell migration on fibronectin
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Zhang, Z.; Morla, A. O.; Vuori, K.; Bauer, J. S.; Juliano, R. L.; Ruoslahti, E. The alpha v beta 1 integrin functions as a fibronectin receptor but does not support fibronectin matrix assembly and cell migration on fibronectin. J. Cell Biol. 1993, 122, 235-242.
-
(1993)
J. Cell Biol.
, vol.122
, pp. 235-242
-
-
Zhang, Z.1
Morla, A.O.2
Vuori, K.3
Bauer, J.S.4
Juliano, R.L.5
Ruoslahti, E.6
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25
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0027245866
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Functional role of the cytoplasmic domain of the integral alpha 5 subunit
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Bauer, J. S.; Varner, J.; Schreiner, C.; Kornberg, L.; Nicholas, R.; Juliano, R. L. Functional role of the cytoplasmic domain of the integral alpha 5 subunit. J. Cell Biol. 1993, 122, 209-221.
-
(1993)
J. Cell Biol.
, vol.122
, pp. 209-221
-
-
Bauer, J.S.1
Varner, J.2
Schreiner, C.3
Kornberg, L.4
Nicholas, R.5
Juliano, R.L.6
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26
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1442276953
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note
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The number of attached cells was quantified manually by counting the number of cells in four separate fields of view per sample, and four samples were analyzed per condition. Student's t-tests were used to identify statistically significant differences in attached cell number.
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27
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0025214681
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A novel fibronectin receptor with an unexpected subunit composition (alpha v beta 1)
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Vogel, B. E.; Tarone, G.; Giancotti, F. G.; Gailit, J.; Ruoslahti, E. A novel fibronectin receptor with an unexpected subunit composition (alpha v beta 1). J. Biol. Chem. 1990, 265, 5934-5937.
-
(1990)
J. Biol. Chem.
, vol.265
, pp. 5934-5937
-
-
Vogel, B.E.1
Tarone, G.2
Giancotti, F.G.3
Gailit, J.4
Ruoslahti, E.5
-
28
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0032911020
-
Bidirectional signaling between the cytoskeleton and integrins
-
Schoenwaelder, S. M.; Burridge, K. Bidirectional signaling between the cytoskeleton and integrins. Curr. Opin. Cell. Biol. 1999, 11, 274-286.
-
(1999)
Curr. Opin. Cell. Biol.
, vol.11
, pp. 274-286
-
-
Schoenwaelder, S.M.1
Burridge, K.2
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