Molecular cloning of a Pinguiochrysis pyriformis oleate-specific microsomal Δ12-fatty acid desaturase and functional analysis in yeasts and thraustochytrids

Journal of Biochemistry

Volumn 150, Issue 4, 2011, Pages 375-383

  Matsuda, Takanori ^a   Sakaguchi, Keishi ^a   Kobayashi, Takumi ^a   Abe, Eriko ^a   Kurano, Norihide ^b   Sato, Akira ^b   Okita, Yuji ^c   Sugimoto, Shinichi ^c   Hama, Yoichiro ^d   Hayashi, Masahiro ^e   Okino, Nozomu ^a   Ito, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

^c NIPPON SUISAN KAISHA LTD   (Japan)

^d SAGA UNIVERSITY   (Japan)

^e UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

12 fatty acid desaturase; fatty acid; gene expression; PUFA; thraustochytrid

Indexed keywords

DELTA 12 FATTY ACID DESATURASE; FATTY ACID; ICOSAPENTAENOIC ACID; LINOLEIC ACID; OLEIC ACID; OXYGENASE; POLYUNSATURATED FATTY ACID; UBIQUITIN; UNCLASSIFIED DRUG;

ALGA; AMINO ACID SEQUENCE; ARTICLE; AURANTIOCHYTRIUM LIMACINUM; FATTY ACID SYNTHESIS; GENE EXPRESSION; GENE ISOLATION; LABYRINTHULIDA; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYLOGENETIC TREE; PINGUIOCHRYSIS PYRIFORMIS; PROMOTER REGION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING; YEAST; YEAST CELL;

CLONING, MOLECULAR; FATTY ACID DESATURASES; MICROALGAE; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; SACCHAROMYCES CEREVISIAE; STRAMENOPILES;

ALGAE; PINGUIOCHRYSIS PYRIFORMIS; THRAUSTOCHYTRIIDAE;

EID: 80053496680     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvr076     Document Type: Article

References (29)

1. Vrabilk, M., Prusikova, M., Snejdrlova, M., and Zlattohlavek, L. (2009) Omega-3 fatty acids and cardiovascular disease risk: Do we understand the relationship? Physiol. Res. 58 (Suppl 1), S19-S26
2. von Schacky, C. and Harris, W.S. (2007) Cardiovascular benefits of omega-3 fatty acids. Cardiovasc. Res. 73, 310-3153.
3. Innis, S.M. (2008) Dietary omega 3 fatty acids and the developing brain. Brain Res. 1237, 35-43
4. Lee, J.H., OKeefe, J.H., Lavie, C.J., and Harris, W.S. (2009) Omega-3 fatty acids: cardiovascular benefits, sources and sustainability. Nat. Rev. Cardiol. 6, 753-758
5. Sayanova, O.V. and Napier, J.A. (2004) Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry. 65, 147-158 (Pubitemid 38147352)
6. Raghukumar, S. (2008) Thraustochytrid marine protists: production of PUFAs and other emerging technologies. Mar. Biotechnol. 10, 631-640
7. Kawachi, M., Inouye, I., Honda, D., OKelly, C.J., Bailey, J.C., Bidigare, R.R., and Andersen, R.A. (2002) The pinguiophyceae classis nova, a new class of photosynthetic stramenopiles whose members produce large amounts of omega-3 fatty acids. Phycological Res. 50, 31-47 (Pubitemid 34572953)
8. Taoka, Y., Nagano, N., Okita, Y., Izumida, H., Sugimoto, S., and Hayashi, M. (2009) Influences of culture temperature on the growth, lipid content and fatty acid composition of A. limacinum strain mh0186. Mar. Biotechnol. 11, 368-374
9. Watanabe, M.M., Kawachi, M., Hiroki, M., and Kasai, F. (2000) NIES-collection List of Strains, Microalgae and Protozoa. 6th edn, NIES, Japan
10. Bijli, K.M., Singh, B.P., Sridhara, S., and Arora, N. (2001) Isolation of total RNA from pollens. Prep. Biochem. Biotechnol. 31, 155-162 (Pubitemid 32533332)
11. Chen, D., Yang, B., and Kuo, T. (1992) One-step transformation of yeast in stationary phase. Curr. Genet. 21, 83-84
12. Nakamura, Y., Gojobori, T., and Ikemura, T. (2000) Codon usage tabulated from international DNA sequence databases: Status for the year 2000. Nucleic Acids Res. 28, 292 (Pubitemid 30047783)
13. Qiu, X., Hong, H., and Mackenzie, S.L. (2001) Identification of a delta 4 fatty acid desaturase from Thraustochytrium. sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea. J. Biol. Chem. 276, 31561-31566
14. Abe, E., Hayashi, Y., Hama, Y., Hayashi, M., Inagaki, M., and Ito, M. (2006) A novel phosphatidylcholine which contains pentadecanoic acid at sn-1 and docosahexaenoic acid at sn-2 in Schizochytrium sp. F26-b. J. Biochem. 140, 247-253 (Pubitemid 44618911)
15. Dubois, N., Barthomeuf, C., and Berge, J.P. (2006) Convenient preparation of picolinyl derivatives from fatty acid esters. Eur. J. Lipid Sci. Technol. 108, 28-32 (Pubitemid 43198332)
16. Sakuradani, E., Kobayashi, M., Ashikari, T., and Shimizu, S. (1999) Identification of delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae. Eur. J. Biochem. 261, 812-820 (Pubitemid 29226080)
17. Passorn, S., Laoteng, K., Rachadawong, S., Tanticharoen, M., and Cheevadhanarak, S. (1999) Heterologous expression of Mucor rouxii delta(12)-desaturase gene in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 263, 47-51 (Pubitemid 29443637)
18. Wei, D., Li, M., Zhang, X., Ren, Y., and Xing, L. (2004) Identification and characterization of a novel delta12-fatty acid desaturase gene from Rhizopus arrhizus. FEBS Lett. 573, 45-50 (Pubitemid 39141033)
19. Pereira, S.L., Huang, Y.S., Bobic, E.G., Kinney, A.J., Stecca, K.L., Packer, J.C., and Mukerji, P. (2004) A novel omega 3-fatty acid desaturase involved in the biosynthesis of eicosapentaenoic acid. Biochem. J. 378, 665-671 (Pubitemid 38367257)
20. Petrini, G.A., Altabe, S.G., and Uttaro, A.D. (2004) Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor. Eur. J. Biochem. 271, 1079-1086 (Pubitemid 38373041)
21. Shanklin, J., Whittle, E., and Fox, B.G. (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry. 33, 12787-12794 (Pubitemid 24352868)
22. Felsenstein, J. (1996) Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods. Methods Enzymol. 266, 418-427
23. Orikasa, Y., Yamada, A., Yu, R., Itoh, Y., Nishida, T., Yumoto, I., Watanabe, K., and Okuyama, H. (2004) Characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738. Cell Mol. Biol. 50, 625-630
24. Metz, J.G., Roessler, P., Facciotti, D., Levering, C., Dittrich, F., Lassner, M., Valentine, R., Lardizabal, K., Domergue, F., Yamada, A., Yazawa, K., Knauf, V., and Browse, J. (2001) Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293, 290-293 (Pubitemid 32694743)
25. Napier, A.J. and Michaelson, L.V. (2001) Genomic and functional characterization of polyunsaturated fatty acid biosynthesis in Caenorhabditis elegans. Lipids 36, 761-766 (Pubitemid 32924102)
26. Nakamura, M.T. and Nara, T.Y. (2003) Essential fatty acid synthesis and its regulation in mammals. Prostaglandins Leukot. Essent. Fatty Acids 68, 145-150 (Pubitemid 36216478)
27. Lippmeier, J.C., Crawford, K.C., Owen, C.B., Rivas, A.A., Metz, J.G., and Apt, K.E. (2009) Characterization of both polyunsaturated fatty acid biosynthetic pathways in Schizochytrium sp. Lipids 44, 621-630
28. Jae-Cheol, L., Periasamy, A., Won-Ho, K., Myung-ju, N., Su-Jin, L., Jeong-Woo, S., and Byung-Ki, H. (2008) Identification of -9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris. Biotechnol. Bioprocess Engineer 13, 524-532
29. Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T.J., Higgins, D.J., and Thompson, J.D. (2003) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 31, 3497-3500 (Pubitemid 37442184)