Identification of Δ12-fatty acid desaturase from arachidonic acid- producing Mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae

European Journal of Biochemistry

Volumn 261, Issue 3, 1999, Pages 812-820

  Sakuradani, Eiji ^c   Kobayashi, Michihiko ^b   Ashikari, Toshihiko ^a   Shimizu, Sakayu ^c  

^a SUNTORY LTD   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c NONE

Author keywords

12 desaturase; Cloning; Fungi; Gene expression

Indexed keywords

ACYL COENZYME A DESATURASE; ARACHIDONIC ACID; COMPLEMENTARY DNA; LINOLEIC ACID; LINOLENIC ACID; MICROSOME ENZYME; OLEIC ACID; DELTA 12 FATTY ACID DESATURASE; DELTA-12 FATTY ACID DESATURASE; PRIMER DNA;

AMINO ACID SEQUENCE; ARACHIDONIC ACID METABOLISM; ARTICLE; ASPERGILLUS ORYZAE; ENZYME ASSAY; EVOLUTION; FATTY ACID DESATURATION; LIPID COMPOSITION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SOYBEAN; YEAST; ZYGOMYCETES; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; MORTIERELLA; SOUTHERN BLOTTING;

ASPERGILLUS ORYZAE; BRASSICA NAPUS; CAENORHABDITIS ELEGANS; GLYCINE MAX; MORTIERELLA; MORTIERELLA ALPINA; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; ARACHIDONIC ACID; ASPERGILLUS ORYZAE; BASE SEQUENCE; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; FATTY ACID DESATURASES; MOLECULAR SEQUENCE DATA; MORTIERELLA; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0033125454     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00333.x     Document Type: Article

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