A culture model for neurite regeneration of human spinal cord neurons

Journal of Neuroscience Methods

Volumn 201, Issue 2, 2011, Pages 346-354

  Koechling, T ^a,b,c   Khalique, H ^a   Sundstrom E ^d   Avila J ^b,c   Lim, F ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOA   (Spain)

^c CENTRO DE INVESTIGACIÓN BIOMÉDICA EN RED SOBRE ENFERMEDADES NEURODEGENERATIVAS CIBERNED   (Spain)

^d KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Brain derived neurotrophic factor; Human neural precursors; Human neurite regeneration in culture; Postmitotic human neurons

Indexed keywords

BRAIN DERIVED NEUROTROPHIC FACTOR; GROWTH HORMONE; SOMATOMEDIN C;

ARTICLE; CELL DENSITY; CELL VIABILITY; CONTROLLED STUDY; FETUS; HUMAN; HUMAN CELL; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE REGENERATION; NEURITE; PRIORITY JOURNAL; SPINAL CORD INJURY; SPINAL CORD NERVE CELL;

CELL CULTURE TECHNIQUES; DRUG EVALUATION, PRECLINICAL; FETUS; HUMANS; NERVE GROWTH FACTORS; NERVE REGENERATION; NEURAL STEM CELLS; NEURITES; SPHEROIDS, CELLULAR; SPINAL CORD;

EID: 80053215115     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.08.024     Document Type: Article

References (44)

1. Abramoff M.D., Magelhaes P.J., Ram S.J. Image processing with image. J Biophot Int 2004, 11:36-42.
2. Åkesson E., Piao J.H., Samuelsson E.B., Holmberg L., Kjaeldgaard A., Falci S., Sundström E., Seiger A. Long-term culture and neuronal survival after intraspinal transplantation of human spinal cord-derived neurospheres. Physiol Behav 2007, 92:60-66.
3. Akhtar A.Z., Pippin J.J., Sandusky C.B. Animal models in spinal cord injury: a review. Rev Neurosci 2008, 19:47-60.
4. Bradbury E.J., King V.R., Simmons L.J., Priestley J.V., McMahon S.B. NT-3, but not BDNF, prevents atrophy and death of axotomized spinal cord projection neurons. Eur J Neurosci 1998, 10:3058-3068.
5. Chen J.K., Taipale J., Young K.E., Maiti T., Beachy P.A. Small molecule modulation of smoothened activity. Proc Natl Acad Sci USA 2002, 99:14071-14076.
6. Devesa J., Casteleiro N., Rodicio C., Lopez N., Reimunde P. Growth hormone deficiency and cerebral palsy. Ther Clin Risk Manag 2010, 6:413-418.
7. Devesa J., Reimunde P., Devesa A., Souto S., Lopez-Amado M., Devesa P., Arce V.M. Recovery from neurological sequelae secondary to oncological brain surgery in an adult growth hormone-deficient patient after growth hormone treatment. J Rehabil Med 2009, 41:775-777.
8. Dill J., Wang H., Zhou F., Li S. Inactivation of glycogen synthase kinase 3 promotes axonal growth and recovery in the CNS. J Neurosci 2008, 28:8914-8928.
9. Fryer R.H., Kaplan D.R., Kromer L.F. Truncated trkB receptors on nonneuronal cells inhibit BDNF-induced neurite outgrowth in vitro. Exp Neurol 1997, 148:616-627.
10. Goldberg J.L., Espinosa J.S., Xu Y., Davidson N., Kovacs G.T., Barres B.A. Retinal ganglion cells do not extend axons by default: promotion by neurotrophic signaling and electrical activity. Neuron 2002, 33:689-702.
11. Homma K., Koriyama Y., Mawatari K., Higuchi Y., Kosaka J., Kato S. Early downregulation of IGF-I decides the fate of rat retinal ganglion cells after optic nerve injury. Neurochem Int 2007, 50:741-748.
12. Hopkins J.M., Bunge R.P. Regeneration of axons from adult human retina in vitro. Exp Neurol 1991, 112:243-251.
13. Jensen J.B., Parmar M. Strengths and limitations of the neurosphere culture system. Mol Neurobiol 2006, 34:153-161.
14. Jiao J., Huang X., Feit-Leithman R.A., Neve R.L., Snider W., Dartt D.A., Chen D.F. Bcl-2 enhances Ca(2+) signaling to support the intrinsic regenerative capacity of CNS axons. EMBO J 2005, 24:1068-1078.
15. Kato A.C., Touzeau G., Bertrand D., Bader C.R. Human spinal cord neurons in dissociated monolayer cultures: morphological, biochemical, and electrophysiological properties. J Neurosci 1985, 5:2750-2761.
16. Leinninger G.M., Feldman E.L. Insulin-like growth factors in the treatment of neurological disease. Endocr Dev 2005, 9:135-159.
17. Liebl D.J., Huang W., Young W., Parada L.F. Regulation of Trk receptors following contusion of the rat spinal cord. Exp Neurol 2001, 167:15-26.
18. Lilley S., Robbins J. The rat retinal ganglion cell in culture: an accessible CNS neurone. J Pharmacol Toxicol Methods 2005, 51:209-220.
19. Meijering E., Jacob M., Sarria J-CF, Steiner P., Hirling H., Unser M. Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images. Cytometry A 2004, 58:167-176.
20. Mitchell P.J., Hanson J.C., Quets-Nguyen A.T., Bergeron M., Smith R.C. A quantitative method for analysis of in vitro neurite outgrowth. J Neurosci Methods 2007, 164:350-362.
21. Neumann S., Woolf C.J. Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron 1999, 23:83-91.
22. Park K.K., Liu K., Hu Y., Smith P.D., Wang C., Cai B., Xu B., Connolly L., Kramvis I., Sahin M., He Z. Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science 2008, 322:963-966.
23. Piao J.H., Odeberg J., Samuelsson E.B., Kjaeldgaard A., Falci S., Seiger A., Sundström E., Åkesson E. Cellular composition of long-term human spinal cord- and forebrain-derived neurosphere cultures. J Neurosci Res 2006, 84:471-482.
24. Quinn S.M., Walters W.M., Vescovi A.L., Whittemore S.R. Lineage restriction of neuroepithelial precursor cells from fetal human spinal cord. J Neurosci Res 1999, 57:590-602.
25. Reynolds B.A., Rietze R.L. Neural stem cells and neurospheres-re-evaluating the relationship. Nat Methods 2005, 2:333-336.
26. Sánchez Martin C., Díaz-Nido J., Avila J. Regulation of a site-specific phosphorylation of the microtubule-associated protein 2 during the development of cultured neurons. Neuroscience 1998, 87:861-870.
27. Schwab M.E. Repairing the injured spinal cord. Science (New York, NY) 2002, 295:1029-1031.
28. Sebert M.E., Shooter E.M. Expression of mRNA for neurotrophic factors and their receptors in the rat dorsal root ganglion and sciatic nerve following nerve injury. J Neurosci Res 1993, 36:357-367.
29. Smith R.A., Melmed S., Sherman B., Frane J., Munsat T.L., Festoff B.W. Recombinant growth hormone treatment of amyotrophic lateral sclerosis. Muscle Nerve 1993, 16:624-633.
30. Song X.Y., Li F., Zhang F.H., Zhong J.H., Zhou X.F. Peripherally-derived BDNF promotes regeneration of ascending sensory neurons after spinal cord injury. PloS One 2008, 3:e1707.
31. Steeves J.D., Tetzlaff W. Engines, accelerators, and brakes on functional spinal cord repair. Ann NY Acad Sci 1998, 860:412-424.
32. Sternberger L.A., Sternberger N.H. Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ. Proc Natl Acad Sci USA 1983, 80:6126-6130.
33. Sun Y., Pollard S., Conti L., Toselli M., Biella G., Parkin G., Willatt L., Falk A., Cattaneo E., Smith A. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Mol Cell Neurosci 2008, 38:245-258.
34. Takano M., Horie H., Iijima Y., Dezawa M., Sawada H., Ishikawa Y. Brain-derived neurotrophic factor enhances neurite regeneration from retinal ganglion cells in aged human retina in vitro. Exp Eye Res 2002, 74:319-323.
35. Tonges L., Ostendorf T., Lamballe F., Genestine M., Dono R., Koch J.C., Bahr M., Maina F., Lingor P. Hepatocyte growth factor protects retinal ganglion cells by increasing neuronal survival and axonal regeneration in vitro and in vivo. J Neurochem 2011, 117:892-903.
36. Tonra J.R., Curtis R., Wong V., Cliffer K.D., Park J.S., Timmes A., Nguyen T., Lindsay R.M., Acheson A., DiStefano P.S. Axotomy upregulates the anterograde transport and expression of brain-derived neurotrophic factor by sensory neurons. J Neurosci 1998, 18:4374-4383.
37. von Bartheld C.S. Axonal transport and neuronal transcytosis of trophic factors, tracers, and pathogens. J Neurobiol 2004, 58:295-314.
38. Widenfalk J., Lundstromer K., Jubran M., Brene S., Olson L. Neurotrophic factors and receptors in the immature and adult spinal cord after mechanical injury or kainic acid. J Neurosci 2001, 21:3457-3475.
39. Woolf C.J. No Nogo: now where to go?. Neuron 2003, 38:153-156.
40. Yiu G., He Z. Glial inhibition of CNS axon regeneration. Nat Rev Neurosci 2006, 7:617-627.
41. Zhang S.C., Wernig M., Duncan I.D., Brustle O., Thomson J.A. In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 2001, 19:1129-1133.
42. Zhang X.Q., Zhang S.C. Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells. Methods Mol Biol 2010, 584:355-366.
43. Zhou X.F., Rush R.A. Endogenous brain-derived neurotrophic factor is anterogradely transported in primary sensory neurons. Neuroscience 1996, 74:945-953.
44. Ziegler L., Segal-Ruder Y., Coppola G., Reis A., Geschwind D., Fainzilber M., Goldstein R.S. A human neuron injury model for molecular studies of axonal regeneration. Exp Neurol 2010, 223:119-127.