The "Bringer" strategy: A very fast and highly efficient method for construction of mutant libraries by error-prone polymerase chain reaction of ring-closed plasmids

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 117, Issue 2, 2004, Pages 115-122

  Bichet, Andreas ^a   Bureik, Matthias ^a   Lenz, Natalie ^a   Bernhardt, Rita ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

peptide; Bringer strategy; Error prone polymerase chain reaction; Escherichia coli; Ring closed plasmids

Indexed keywords

BETA LACTAMASE; DNA FRAGMENT;

ANALYTIC METHOD; ARTICLE; BACTERIAL GENETICS; BIOCATALYST; CONTROLLED STUDY; DNA LIBRARY; ENZYME BINDING; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CONSTRUCT; GENE EXPRESSION; GENE MUTATION; GENETIC CODE; GENETIC SCREENING; GENETIC TRANSFORMATION; MOLECULAR CLONING; MOLECULAR EVOLUTION; MUTAGENESIS; MUTANT; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; REACTION ANALYSIS;

ANIMALS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE LIBRARY; MUTAGENESIS; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI; VECTORS;

EID: 2942741205     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1385/ABAB:117:2:115     Document Type: Article

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