Marker-Free Chromosomal Expression of Foreign and Native Genes in Escherichia coli

Methods in Molecular Biology

Volumn 765, Issue , 2011, Pages 113-123

  Chiang, Chung Jen ^a   Chen, Po Ting ^b   Chen, Shan Yu ^c   Chao, Yun Peng ^d  

^a CHINA MEDICAL UNIVERSITY   (Taiwan)

^b SOUTHERN TAIWAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

^c YUAN ZE UNIVERSITY   (Taiwan)

^d FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Flippase; Genomic engineering; Lycopene; Markerless; Recombinase

Indexed keywords

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENOME; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METHODOLOGY;

CHROMOSOMES, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; GENES; GENES, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; GENOME, BACTERIAL; MUTAGENESIS, INSERTIONAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 80052932992     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-197-0_8     Document Type: Chapter

References (14)

1. Jones K. L., Kim S. W., and Keasling J. D. (2000) Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria. Metab. Eng. 2, 328–38.
2. Peredelchuk M. Y. and Bennett G. N. (1997) A method for construction of E. coli strains with multiple DNA insertions in the chromosome. Gene 187, 231–238.
3. Julian A., Hanak J. and Cranenburgh R. M. (2001) Antibiotic-free plasmid selection and maintenance in bacteria, In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: A Comparative View on Host Physiology (Merten, O.-W., Mattanovich, D., Lang, C., Larsson, G., Neubauer, P., Porro, D., Postma, P., Teixeira de Mattos, J., and Cole, J. A. ed.). Kluwer Academic, Dordrecht, Netherlands, pp 121–134.
4. Haldimann A. and Wanner B. L. (2001) Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183, 6384–6393.
5. Chiang C. J., Cheng P. T. and Chao Y. P. (2008) Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli. Biotechnol. Bioeng. 101, 985–995.
6. Tabor S. and Richardson C. C. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA. 82, 1074–1078.
7. Studier F. W. and Moffatt B. A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113–130.
8. Imburgio D., Rong M., Ma K. and McAllister W. T. (2000) Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochem. 39, 10419–10430.
9. Alper H., Fischer C., Nevoigt E. And Stephanopoulos G. (2005) Tuning genetic control through promoter engineering. Proc. Natl. Acad. Sci. USA. 102, 12678–12683.
10. Meynial-Salles I., Cervin M. A. and Soucaille P. (2005) New tool for metabolic pathway engineering in Escherichia coli: one-step method to modulate expression of chromosomal genes. Appl. Environ. Microbiol. 71, 2140–2144.
11. Datsenko K. A. and Wanner B. L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA. 97, 6640–6645.
12. Wang C. W., Oh M. K. and Liao J. C. (1999) Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. Biotechnol Bioeng. 62, 235–241.
13. Perry K. L., Simonitch T. A., Harrison-Lavoie K. J. and Liu S. T. (1986) Cloning and regulation of Erwinia herbicola pigment genes. J. Bacteriol. 168, 607–612.
14. Wang Z. W., Law W. S. and Chao Y. P. (2004) Improvement of the thermoregulated T7 expression system by using the heat-sensitive lacI. Biotechnol Prog. 20, 1352–1358.