An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences

PLoS ONE

Volumn 6, Issue 9, 2011, Pages

  Pollier, Jacob ^a,b   González Guzmán, Miguel ^a,b,c   Ardiles Diaz, Wilson ^a,b   Geelen, Danny ^b   Goossens, Alain ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c Universitat Politècnica de València   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PLANT DNA;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ARTICLE; COLONY HYBRIDIZATION; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA LIBRARY; DNA SCREENING; DNA TRANSCRIPTION; GENETIC CODE; GENETIC IDENTIFICATION; GENETIC SELECTION; GINSENG; GLYCYRRHIZA GLABRA; MAESA LANCEOLATA; MEDICINAL PLANT; MOLECULAR CLONING; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE TAGGED SITE; AMINO ACID SEQUENCE; DNA SEQUENCE; EXPRESSED SEQUENCE TAG; GENE EXPRESSION PROFILING; GENE LIBRARY; GENETICS; GLYCYRRHIZA; HYBRIDIZATION; MOLECULAR GENETICS; NUCLEIC ACID HYBRIDIZATION; PANAX; PLANT GENE; PLANT GENOME; PRIMULACEAE;

AMINO ACID SEQUENCE; AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, PLANT; EXPRESSED SEQUENCE TAGS; GENE EXPRESSION PROFILING; GENE LIBRARY; GENES, PLANT; GENOME, PLANT; GLYCYRRHIZA; HYBRIDIZATION, GENETIC; MOLECULAR SEQUENCE DATA; MYRSINACEAE; NUCLEIC ACID HYBRIDIZATION; OPEN READING FRAMES; PANAX; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 80052854088     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0024978     Document Type: Article

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