Targeted rapid amplification of cDNA ends (T-RACE) - An improved RACE reaction through degradation of non-target sequences

Nucleic Acids Research

Volumn 38, Issue 21, 2010, Pages

  Bower, Neil I ^a   Johnston, Ian A ^a  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DOUBLE STRANDED DNA; URACIL DNA GLYCOSIDASE;

ARTICLE; CONTROLLED STUDY; DNA DEGRADATION; DNA PURIFICATION; DNA SEQUENCE; DNA SYNTHESIS; DNA TEMPLATE; DNA TRANSCRIPTION; NICK END LABELING; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TARGETED RAPID AMPLIFICATION OF CDNA ENDS;

ANIMALS; BASE SEQUENCE; DEOXYURACIL NUCLEOTIDES; DNA, COMPLEMENTARY; POLYMERASE CHAIN REACTION; SALMO SALAR; TEMPLATES, GENETIC; URACIL-DNA GLYCOSIDASE; ZEBRAFISH;

EID: 78649831851     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkq816     Document Type: Article

References (12)

1. Salehi-Ashtiani, K., Lin, C. W., Hao, T., Shen, Y., Szeto, D., Yang, X. P., Ghamsari, L., Lee, H., Fan, C. Y., Murray, R. R. et al. (2009) Large-scale RACE approach for proactive experimental definition of C. elegans ORFeome. Genome Res., 19, 2334-2342.
2. Frohman, M. A., Dush, M. K. and Martin, G. R. (1988) Rapid production of full-length cDNAs from rare transcripts- amplification using a single gene-specific oligonucleotide primer. Proc. Natl Acad. Sci. USA, 85, 8998-9002.
3. 0 termini of mRNAs. Nucleic Acids Res., 21, 1683-1684.
4. 0 CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs. Nucleic Acids Res., 27, e31.
5. 0-RACE approach. Nucleic Acids Res., 28, e96.
6. Schaefer, B. C. (1995) Revolutions in rapid amplification of cDNA ends- new strategies for polymerase chain-reaction cloning of full-length cDNA ends. Anal. Biochem., 227, 255-273.
7. Lipovich, L., Lynch, E. D., Lee, M. K. and King, M. C. (2001) A novel sodium bicarbonate cotransporter-like gene in an ancient duplicated region: SLC4A9 at 5q31. Genome Biol., 2, research0011. 1-0011. 13.
8. Aslanzadeh, J. (2004) Preventing PCR amplification carryover contamination in a clinical laboratory. Ann. Clin. Lab. Sci., 34, 389-396.
9. Longo, M. C., Berninger, M. S. and Hartley, J. L. (1990) Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain-reactions. Gene, 93, 125-128.
10. Buchman, G. W., Schuster, D. M. and Rashtchian, A. (1993) Selective Rna amplification- a novel method using dump-containing primers and uracil DNA glycosylase. PCR Methods Appl., 3, 28-31.
11. 0 end cDNA amplification using classic RACE. Nat. Protoc., 1, 2555-2562.
12. Olivarius, S., Plessy, C. and Carninci, P. (2009) High-throughput verification of transcriptional starting sites by Deep-RACE. BioTechniques, 46, 130-132.