Full-length cDNA cloning and determination of mRNA 5' and 3' ends by amplication of adaptor-ligated cDNA

BioTechniques

Volumn 21, Issue 3, 1996, Pages 526-534

  Chenchik, A ^a   Diachenko, L ^a   Moqadam, F ^a   Tarabykin, Y ^b   Lukyanov, S ^b   Siebert, P D ^a  

^a CLONTECH Laboratories Inc   (United States)

^b SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; COMPLEMENTARY DNA; DOUBLE STRANDED DNA; MESSENGER RNA; POLYADENYLATED RNA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; SOMATOMEDIN B; TRANSFERRIN RECEPTOR;

GENE AMPLIFICATION; HUMAN; MOLECULAR CLONING; PLACENTA; POLYMERASE CHAIN REACTION; REVIEW;

EID: 0029812093     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213pf02     Document Type: Review

References (29)

1. Adams, M.D., M.B. Soares, A.R. Kerlavage, C. Field and J.C. Venter. 1993. Rapid cDNA sequencing (expressed sequencing tags) from a directionally cloned human infant brain cDNA library. Nature Genet. 4:373-380.
2. Arnold, C. and I.J. Hogson. 1991. Vectorette PCR: a novel approach to genome walking. PCR Methods Appl. 1:39-42.
3. Barnes, W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage template. Proc. Natl. Acad. Sci. USA 91:2216-2220.
4. Bertling, W.M., F. Beier and E. Reichenberger. 1993. Determination of 5′ ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Appl. 3:95-99.
5. Bertrand, E., M. Fromont-Racine, R. Pictet and T. Grande. 1993. Visualization of the interaction of a regulatory protein with RNA in vivo. Proc. Natl. Acad. Sci. USA 90:3496-3500.
6. Borson, N.D., W.L. Sato and L.R. Drewes. 1992. A lock-docking oligo(dT) primer for 5′ and 3′ RACE PCR. PCR Methods Appl. 2:144-148.
7. Brenman, C.A., A.E. Manthey and R.I. Gumport. 1983. Using T4 RNA ligase with DNA substrates, p. 38-52. In R. Wu (Ed.), Methods in Enzymology, Vol. 100. Academic Press, San Diego.
8. D'Alessio, J.M. and G.F. Gerard. 1988. Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase H: the fate of information at the 5′ terminus and the effect of E. coli DNA ligase. Nucleic Acids Res. 16:1999-2014.
9. Dumas, J.B., M. Edwards, J. Delort and J. Mallet. 1991. Oligonucleotide ligation to ss cDNA: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by in vitro amplification. Nucleic Acids Res. 19:5227-5232.
10. Erba, H.P., P. Gunning and L. Kedes. 1986. Nucleotide sequence of the human γ cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes. Nucleic Acids Res. 14:5275-5294.
11. Frohman, M.A. 1994. On beyond RACE. PCR Methods Appl. 4:S40-S58.
12. Frohman, M.A., M.K. Dush and G.R. Martin. 1988. Rapid production of full-length cDNA from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85:8998-9002.
13. Frohman, M.A. and G.R. Martin. 1989. Rapid amplification of cDNA ends using nested primers. Technique 1:165-170.
14. Gubler, U. and B.J. Hoffman. 1983. A simple and very efficient method for generating complimentary DNA libraries. Gene 25:263-269.
15. Hofmann, M.A. and D.A. Brian. 1991. A PCR enhanced method for determining the 5′ end sequence of the mRNAs. PCR Methods Appl. 1:43-45.
16. Kellogg, D.E., I. Rybalkin, S. Chen, N. Mukhamedova, T. Vlasik, P. Siebert and A. Chenchik. 1994. TaqStart Antibody™: "Hotstart" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques 16:1134-1137.
17. Liang, P. and A. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-970.
18. Lukianov, S., N.K. Gurskaya, K.A.. Lukianov, V.S. Tarabukin and E.D. Sverdlov. 1994. Highly efficient subtractive hybridization of cDNA. Bioorg. Chem. (Russia) 20:701-704.
19. Maruyama, K. and S. Sugano. 1994. Oligo-capping: a simple method to replace the cap structure of eukariotic mRNAs with oligoribonucleotides. Gene 138:171-174.
20. Maunders, M. 1993. DNA and RNA ligases, p. 213-230. In M.M. Burrell (Ed.), Methods of Molecular Biology. Enzymes of Molecular Biology, Vol. 16. Humana Press, Totowa, NJ.
21. Miwa, T., Y. Manabe, K. Kurokawa, S. Kamada, N. Kanda, G. Bruns, H. Ueyama and T. Kakunaga. 1991. Structure, chromosome location, and expression of the human smooth muscle (enteric type) γ-actin gene: evolution of six human actin genes. Mol. Cell. Biol. 11:3296-3306.
22. Morgam, D.O., J.C. Edman, D.N. Standring, V.A. Fried, M.C. Smith, R.A. Roth and W.J. Rutter. 1987. Insulin-like growth factor II receptor as a multifunctional binding protein. Nature 329:301-307.
23. Nakano, Y., T. Nishihara, S. Sasayama, T. Miwa, S. Kamada and T. Kakunaga. 1991. Transcriptional regulatory elements in the 5′ upstream and first intron regions of the human smooth muscle (aortic type) α-actin encoding gene. Gene 99:285-289.
24. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Siebert, P.D., A. Chenchik, D.E. Kellogg, K.A. Lukyanov and S.A. Lukyanov. 1995. An improved method for walking in uncloned genomic DNA. Nucleic Acids Res. 23:1087-1088.
26. Schneider, M.J., M.J. Owen, D. Banville and J.G. Williams. 1984. Primary structure of human transferrin receptor deduced from the mRNA sequence. Nature 311:675-678.
27. Volloch, V., B. Schweitzer and S. Rits. 1994. Ligation-mediated amplification of RNA from murine erythroid cells reveals a novel class of β-globin mRNA with an extended 5′-untranslated region. Nucleic Acids Res. 22:2507-2511.
28. Welsh, J. and M. McClelland. 1991. Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers. Nucleic Acids Res. 19:5275-5279.
29. Welsh, J. and A. McClelland. 1992. Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res. 20:4965-4970.