Purification and biochemical characterization of a moderately halotolerant NADPH dependent xylose reductase from Debaryomyces nepalensis NCYC 3413

Bioresource Technology

Volumn 102, Issue 20, 2011, Pages 9710-9717

  Kumar, Sawan ^a   Gummadi, Sathyanarayana N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

Author keywords

Debaryomyces nepalensis; Halotolerant; Purification; Xylose; Xylose reductase

Indexed keywords

ACTIVE SITE; BIOCHEMICAL CHARACTERIZATION; CATALYTIC EFFICIENCIES; COMMERCIAL PRODUCTIONS; DEBARYOMYCES NEPALENSIS; DIETHYLPYROCARBONATE; HALOTOLERANT; HISTIDINE RESIDUES; IN-SITU; MALDI-TOF/MS; PEPTIDE MASS FINGERPRINTING; SEQUENCE HOMOLOGY; XYLOSE REDUCTASE;

AMINO ACIDS; ENZYME ACTIVITY; PURIFICATION; SODIUM CHLORIDE; SUGAR SUBSTITUTES; YEAST;

ENZYME INHIBITION;

ARABINOSE; DIETHYL PYROCARBONATE; ENZYME; HEXOSE; HISTIDINE; MONOMER; PENTOSE; POTASSIUM CHLORIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SODIUM CHLORIDE; UNCLASSIFIED DRUG; XYLOSE; XYLOSE REDUCTASE;

ALCOHOL; BIOCHEMISTRY; CATALYST; EFFICIENCY MEASUREMENT; ENZYME ACTIVITY; HOMOGENEITY; INDUSTRIAL PRODUCTION; INHIBITION; POTASSIUM CHLORIDE; PURIFICATION; SALINITY TOLERANCE; SODIUM CHLORIDE; SUGAR; YEAST;

ARTICLE; DEBARYOMYCES; DEBARYOMYCES NEPALENSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; SALT TOLERANCE;

ALDEHYDE REDUCTASE; AMINO ACID SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; NADP; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; YEASTS;

DEBARYOMYCES NEPALENSIS;

EID: 80052693031     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2011.07.030     Document Type: Article

References (38)

1. Azuma M., Ikeuchi T., Kiritani R., Kato J., Ooshima H. Increase in xylitol production by Candida tropicalis upon addition of salt. Biomass. Bioenerg. 2000, 19:129-135.
2. Bicho P.A., Runnals P.L., Cunningham J.D., Lee H. Induction of xylose reductase and xylitol dehydrogenase activities in Pachysolen tannophilus and Pichia stipitis on mixed sugars. Appl. Environ. Microbiol. 1988, 54:50-54.
3. Cardoso L.A., Ferreira S.T., Hermes-Lima M. Reductive inactivation of yeast glutathione reductase by Fe(II) and NADPH. Comp. Biochem. Physiol. 2008, 151:313-321.
4. Cecconi I., Moroni M., Vilardo P.G., Dal Monte M., et al. Oxidative modification of aldose reductase induced by copper ion. Factors and conditions affecting the process. Biochemistry 1998, 37:14140-14167.
5. Dennis P.P., Shimmin L.C. Evolutionary divergence and salinity mediated selection in halophilic archaea. Microbiol. Mol. Biol. Rev. 1997, 61:90-104.
6. Dieters W. Xylitol production from d-xylose. Swiss Patent 1975, 560:175.
7. Ditzelmuller G., Kubicek C.P., Wohrer W., Rohr M. Xylose metabolism in Pachysolen tannophilus purification and properties of xylose reductase. Can. J. Microbiol. 1984, 30:1330-1336.
8. Gírio F.M., Fonseca C., Carvalheiro F., Duarte L.C., Marques S., Bogel-Łukasik R. Hemicelluloses for fuel ethanol, A review. Bioresour. Technol. 2010, 101:4775-4800.
9. Gummadi S.N., Kumar D.S. Pectin lyase and pectate lyase from Debaryomyces nepalensis isolated from rotten apple. Res. J. Microbiol. 2006, 1:52-159.
10. Gummadi S.N., Kumar S. Effect of temperature and salts on growth of halotolerant yeast, Debaryomyces nepalensis NCYC 3413. Am. J. Food Technol. 2008, 3:354-360.
11. Gummadi S.N., Kumar S., Aneesh C.N.A. Effect of salt on growth and pectinase production by halotolerant yeast, Debaryomyces nepalensis NCYC 3413. Curr. Microbiol. 2007, 54:472-476.
12. He X.Y., Zhuang Y.H., Zhang X.G., Li G.L. Comparative proteome analysis of culture supernatant proteins of Mycobacterium tuberculosis H37Rv and H37Ra. Microbes Infect. 2003, 5:851-856.
13. Kavanagh K.L., Klimacek M., Nidetzky B., Wilson D.K. The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis. Biochemistry 2002, 41:8785-8795.
14. Kim S.Y., Kim J.H., Oh D.K. Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis. J. Ferment. Bioeng. 1997, 83:267-270.
15. Kratzer R., Wilson D.K., Nidetzky B. Catalytic mechanism and substrate selectivity of aldo-keto reductases, insights from structure-function studies of Candida tenuis xylose reductase. IUBMB Life 2006, 58:499-507.
16. Kuhn A., van Zyl C., van Tonder A., Prior B.A. Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae. Appl. Environ. Microbiol. 1995, 61:1580-1585.
17. Kumar S., Lal P., Gummadi S.N. Growth of food spoiling yeast Debaryomyces nepalensis NCYC 3413 under the influence of pH and salt. Curr. Microbiol. 2008, 57:598-602.
18. Kumar S., Gummadi S.N. Osmotic adaptation in halotolerant yeast, Debaryomyces nepalensis NCYC 3413, role of osmolytes and cation transport. Extremophile 2009, 13:793-805.
19. Kumar S., Gummadi S.N. Metabolism of glucose and xylose as single and mixed feed in Debaryomyces nepalensis NCYC 3413, production of industrially important metabolites. Appl. Microbiol. Biotechnol. 2010, 89:1405-1415.
20. Lee J.K., Koo B.S., Kim S.Y. Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis. Appl. Environ. Microbiol. 2003, 69:6179-6188.
21. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.G. Measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
22. Mayr P., Petschacher B., Nidetzky B. Xylose reductase from the basidiomycete fungus Cryptococcus flavus, purification, steady-state kinetic characterization, and detailed analysis of the substrate binding pocket using structure-activity relationships. J. Biochem. 2003, 133:553-562.
23. Naidu G.S.N., Panda T. Studies on pH and thermal deactivation of pectolytic enzymes from Aspergillus niger. Biochem. Eng. J. 2003, 16:57-67.
24. Neuhauser W., Haltrich D., Kulbe K.D., Nidetzky B. NAD(P)H dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Biochem. J. 1997, 326:683-692.
25. Ostergaard S., Olsson L., Nielsen J. Metabolic engineering of Saccharomyces cerevisiae. Appl. Environ. Microbiol. 2000, 66:34-50.
26. Panagiotou G., Christakopoulos P. NADPH-dependent d-aldose reductases and xylose fermentation in Fusarium oxysporum. J. Biosci. Bioeng. 2004, 97:299-304.
27. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning, A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. second ed.
28. Sampaio F.C., Mantovani H.C., Vieira Passos F.J., et al. Bioconversion of d-xylose to xylitol by Debaryomyces hansenii UFV-170, product formation versus growth. Process Biochem. 2005, 40:3600-3606.
29. Sanchez S., Bravo V., Castro E., Moya A.J., Camacho F. The fermentation of mixtures of d-glucose and d-xylose by Candida shehatae, Pichia stipitis or Pachysolen tannophilus to produce ethanol. J. Chem. Technol. Biotechnol. 2002, 77:641-648.
30. Shevchenko A., Tomas H., Havlis J., Olsen J.V., Mann M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 2006, 1:2856-2860.
31. Su Y., Li W., Zhu W., Yu R., et al. Characterization of xylose reductase from Candida tropicalis immobilized on chitosan bead. Afr. J. Biotechnol. 2010, 9:4954-4965.
32. Verduyn C., van Kleef R., Frank J., Schreuder H., van Dijken J.P., Scheffers W.A. Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis. Biochem. J. 1985, 226:669-677.
33. Veronica V.P., Barber M.J. Kinetic and mechanistic properties of biotin sulfoxide reductase. Biochemistry 2001, 40:1430-1440.
34. Wang X., Fang B., Luo J., Li W., Zhang L. Heterologous expression, purification, and characterization of xylose reductase from Candida shehatae. Biotechnol. Lett. 2007, 29:1409-1412.
35. Woodyer R., Simurdiak M., van der Donk W.A., Zhao H. Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa. Appl. Microbiol. Biotechnol. 2005, 71:1642-1647.
36. Yablochkova E.N., Bolotnikova O.I., Mikhailova N.P., Nemova N.N., Ginak A. The activity of xylose reductase and xylitol dehydrogenase in yeasts. Microbiology 2003, 72:414-417.
37. Yokoyama S., Suzuki T., Kawai K., Horitsu H., Takamizawa K. Purification, characterization and structure analysis of NADPH dependent d-Xylose reductases from Candida tropicalis. J. Ferment. Bioeng. 1995, 79:217-223.
38. Zhang F., Qiao D., Xu H., Liao C., Li S., Yi C. Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis. J. Microbiol. 2009, 47:351-357.