Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1

Applied Microbiology and Biotechnology

Volumn 91, Issue 4, 2011, Pages 1049-1060

  Hesseler, Martin ^a,b   Bogdanović, Xenia ^a   Hidalgo, Aurelio ^b   Berenguer, Jose ^b   Palm, Gottfried J ^a   Hinrichs, Winfried ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Biochemical characterization; Haloalkane dehalogenase; Plesiocystis pacifica; Structure

Indexed keywords

ACTIVE SITE POCKET; ACTIVE SITE RESIDUES; BIOCHEMICAL CHARACTERIZATION; CATALYTIC RESIDUE; DEHALOGENASE; FUNCTIONAL EXPRESSION; MONOMERIC PROTEINS; PACIFICA; PH OPTIMA; RELATIVE POSITIONS; SEQUENCE COMPARISONS; SUBSTRATE SPECIFICITY; THREE DIMENSIONAL (3D) STRUCTURES; XANTHOBACTER AUTOTROPHICUS;

CLONING; ESCHERICHIA COLI; PARAFFINS; STRUCTURAL ANALYSIS; THREE DIMENSIONAL; X RAY CRYSTALLOGRAPHY;

ENZYMES;

1 BROMOBUTANE; ASPARTIC ACID; BACTERIAL ENZYME; HALIDE; HALOALKANE DEHALOGENASE; HISTIDINE; HYDROLASE; MONOMER; TRYPTOPHAN; UNCLASSIFIED DRUG;

BACTERIUM; BIOCHEMICAL COMPOSITION; CATALYSIS; CLONAL GROWTH; COLIFORM BACTERIUM; ENZYME ACTIVITY; MARINE ECOSYSTEM; PROTEIN; SUBSTRATE; THREE-DIMENSIONAL MODELING;

ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CLONING; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; MOLECULAR DOCKING; MOLECULAR WEIGHT; MYXOCOCCALES; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PLESIOCYSTIS PACIFICA; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; TEMPERATURE; X RAY CRYSTALLOGRAPHY; XANTHOBACTER AUTOTROPHICUS;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; ESCHERICHIA COLI; HYDROCARBONS, BROMINATED; HYDROGEN-ION CONCENTRATION; HYDROLASES; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MYXOCOCCALES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; PLESIOCYSTIS PACIFICA; XANTHOBACTER AUTOTROPHICUS;

EID: 80052642327     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3328-x     Document Type: Article

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