Repositioning the catalytic triad aspartic acid of Haloalkane dehalogenase: Effects on stability, kinetics, and structure

Biochemistry

Volumn 36, Issue 31, 1997, Pages 9571-9580

  Krooshof, Geja H ^a   Kwant, Edwin M ^a   Damborský, Jiří ^b   Koča, Jaroslav ^b   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b MASARYK UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DEHALOGENATION; ENZYME CONFORMATION; ENZYME STABILITY; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; XANTHOBACTER;

AMINO ACID SEQUENCE; BINDING SITES; BROMIDES; CATALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; ETHYLENE DIBROMIDE; GRAM-NEGATIVE AEROBIC BACTERIA; HYDROLASES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); FELIS CATUS; XANTHOBACTER;

EID: 0030806487     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi971014t     Document Type: Article

References (34)

1. Cheah, E., Austin, C., Ashley, G. W., & Ollis, D. L. (1993) Protein Eng. 6, 575-583.
2. Chen, Y. W., Fersht, A. R., & Henrick, K. (1993) J. Mol. Biol. 234, 1158-1170.
3. Damborský, J., Kutý, M., Němec, M., & Koča, J. (1997) in QSAR in Environmental Sciences VII (Chen, F., & Schuurmann, G., Eds.) SETAC Press, Pensacola, FL.
4. Fersht, A. R. (1985) in Enzyme Structure and Mechanism, W. H. Freeman and Co., New York.
5. Huang, C. Y. (1979) Methods Enzymol. 63, 54-84.
6. Janssen, D. B., Pries, F., van der Ploeg, J., Kazemier, B., Terpstra, P., & Witholt, B. (1989) J. Bacteriol. 171, 6791-6799.
7. Kawasaki, H., Tsuda, K., Matsushita, I., & Tonomura, K. (1992) J. Gen. Microbiol. 138, 1317-1323.
8. Keuning, S., Janssen, D. B., & Witholt, B. (1985) J. Bacteriol. 163, 635-639.
9. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.
10. Kulakova, A. N., Larkin, M. J., & Kulakov, L. A. (1997) Microbiology 143, 109-115.
11. Kumar, A., Sekharudu, C., Ramakrishnan, B., Dupureur, C. M., Zhu, H., Tsai, M.-D., & Sundaralingam, M. (1994) Protein Sci. 3, 2082-2088.
12. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 488-492.
13. Leatherbarrow, R. J. (1987) ENZFITTER: A non-linear regression data analysis program for the IBM PC, Elsevier Science Publishers BV, Amsterdam.
14. Li, Y., & Tsai, M.-D. (1993) J. Am. Chem. Soc. 115, 8523-8526.
15. Mendes, P. (1993) Comput. Appl. Biosci. 9, 563-571.
16. Nagata, Y., Nariya, T., Ohtomo, R., Fukuda, M., Yano, K., & Takagi, M. (1993) J. Bacteriol. 175, 6403-6410.
17. Nobbs, T. J., Cortes, A., Gelpi, J. L., Holbrook, J. J., Atkinson, T., Scawen, M. D., & Nicholls, D. J. (1994) Biochem. J. 300, 491-499.
18. Ollis, D. L., Cheah, E., Cygler, M., Dijkstra, B. W., Frolow, F., Franken, S. M., Haral, M., Remington, S. J., Silman, I., Schrag, J., Sussman, J. L., Verschueren, K. H. G., & Goldman, A. (1992) Protein Eng. 5, 197-211.
19. Ordentlich, A., Kronman, C., Barak, D., Stein, D., Ariel, N., Marcus, D., Velan, B., & Shafferman, A. (1993) FEBS Lett. 334, 215-220.
20. Pries, F., Kingma, J., Pentenga, M., van Pouderoyen, G., Jeronimus-Stratingh, C. M., Bruins, A. P., & Janssen, D. B. (1994) Biochemistry 33, 1242-1247.
21. Pries, F., Kingma, J., Krooshof, G. H., Jeronimus-Stratingh, C. M., Bruins, A. P., & Janssen, D. B. (1995) J. Biol. Chem. 270, 10405-10411.
22. Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989) in Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Sanger, F., Nicklen, S., & Coulsen, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467.
24. Schanstra, J. P., & Janssen, D. B. (1996) Biochemistry 35, 5624-5632.
25. Schanstra, J. P., Rink, R., Pries, F., & Janssen, D. B. (1993) Protein Expression Purif. 4, 479-489.
26. Schanstra, J. P., Kingma, J., & Janssen, D. B. (1996) J. Biol. Chem. 271, 14747-14753.
27. Schmid, F. M. (1990) in Protein Structure - A Practical Approach (Creighton, T. E., Ed.) pp 276-285, IRL Press at Oxford University Press, Oxford, England.
28. Schrag, J. D., Winkler, F. K., & Cygler, M. (1992) J. Biol. Chem. 267, 4300-4303.
29. Schrag, J. D., Vernet, T., Laramée, L., Thomas, D. Y., Recktenwald, A., Okoniewska, M., Ziomek, E., & Cygler, M. (1994) Protein Eng. 8, 835-842.
30. Sprang, S., Standing, T., Fletterick, R. J., Stroud, R. M., Finer-Moore, J., Xuong, N.-H., Hamlin, R., Rutter, W. J., & Craik, C. S. (1987) Science 237, 905-913.
31. Studier, F. W., Rosenberg, A. H., Dunn, J. J., & Dubendorff, J. W. (1990) Methods Enzymol. 185, 60-89.
32. Verschueren, K. H. G., Franken, S. M., Rozeboom, H. J., Kalk, K. H., & Dijkstra, B. W. (1993a) J. Mol. Biol. 232, 856-872.
33. Verschueren, K. H. G., Seljée, F., Rozeboom, H. J., Kalk, K. H., & Dijkstra, B. W. (1993b) Nature 363, 693-698.
34. Verschueren, K. H. G., Kingma, J., Rozeboom, H. J., Kalk, K. H., Janssen, D. B., & Dijkstra, B. W. (1993c) Biochemistry 32, 9031-9037.