Biochemical and mutational studies of the Bacillus cereus CECT 5050T formamidase support the existence of a C-E-E-K tetrad in several members of the nitrilase superfamily

Applied and Environmental Microbiology

Volumn 77, Issue 16, 2011, Pages 5761-5769

  Soriano Maldonado, Pablo ^a   Martínez Gómez, Ana Isabel ^a   Andujar Sánchez, Montserrat ^a   Neira, José L ^b,c   Clemente Jiménez, Josefa María ^a   Heras Vázquez, Francisco Javier Las ^a   Rodríguez Vico, Felipe ^a   Martínez Rodríguez, Sergio ^a  

^a UNIVERSITY OF ALMERÍA   (Spain)

^b MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

^c Physics of Complex Systems   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMATIC ACTIVITIES; HELICOBACTER PYLORI; HUMAN PATHOGENS; METABOLIC PATHWAYS; NITRILASE; SEQUENCE ANALYSIS;

BACILLI; BACTERIOLOGY; CLONING;

BACILLUS CEREUS;

AMIDASE; BACTERIAL PROTEIN; FORMAMIDASE; GLUTAMIC ACID; HYDROLASE; NITRILASE; RECOMBINANT PROTEIN;

ALIPHATIC HYDROCARBON; AMMONIA; BIOCHEMICAL COMPOSITION; ENZYME ACTIVITY; HOMOLOGY; IDENTIFICATION METHOD; KNOWLEDGE; METABOLISM; MUTATION; NITROGEN; NUMERICAL MODEL; PATHOGENICITY; PROTEIN; PURIFICATION; RECOMBINATION;

ARTICLE; BACILLUS CEREUS; CATALYSIS; CHEMISTRY; CIRCULAR DICHROISM; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PHYLOGENY; PROTEIN SECONDARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMIDOHYDROLASES; AMINOHYDROLASES; BACILLUS CEREUS; BACTERIAL PROTEINS; BASE SEQUENCE; CATALYSIS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; CLONING, MOLECULAR; ENZYME ACTIVATION; ENZYME ASSAYS; ESCHERICHIA COLI; GLUTAMIC ACID; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHYLOGENY; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

BACILLUS CEREUS; HELICOBACTER PYLORI;

EID: 80052639748     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00312-11     Document Type: Article

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