Bioluminescence imaging captures the expression and dynamics of endogenous p21 Promoter activity in living mice and intact cells

Molecular and Cellular Biology

Volumn 31, Issue 18, 2011, Pages 3759-3772

  Tinkum, Kelsey L ^a,b   Marpegan, Luciano ^a   White, Lynn S ^a,b   Sun, Jinwu ^a,b   Herzog, Erik D ^a   Piwnica Worms, David ^a,b   Piwnica Worms, Helen ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR 1; CYCLIN DEPENDENT KINASE INHIBITOR 1A; LUCIFERASE; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN P53;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOLUMINESCENCE; CELL CYCLE PHASE; CELL CYCLE PROGRESSION; CELLULAR STRESS RESPONSE; CONTROLLED STUDY; GENE ACTIVATION; GENE LOCUS; GENOTOXICITY; HOMOLOGOUS RECOMBINATION; IN VIVO STUDY; IONIZING RADIATION; LASER CAPTURE MICRODISSECTION; MOLECULAR DYNAMICS; MOLECULAR IMAGING; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REAL TIME POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL CYCLE; CELLS, CULTURED; CYCLIN-DEPENDENT KINASE INHIBITOR P21; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GENE EXPRESSION REGULATION; GENE KNOCK-IN TECHNIQUES; HEPATOCYTES; LUCIFERASES, FIREFLY; MICE; MICE, INBRED C57BL; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RADIATION, IONIZING; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSCRIPTIONAL ACTIVATION; TUMOR SUPPRESSOR PROTEIN P53;

ANIMALIA; MUS;

EID: 80052586742     PISSN: 02707306     EISSN: 10985549     Source Type: Journal    
DOI: 10.1128/MCB.05243-11     Document Type: Article

References (38)

1. Abbas, T., and A. Dutta. 2009. p21 in cancer: intricate networks and multiple activities. Nat. Rev. Cancer 9:400-414.
2. Abramoff, M. D., P. J. Magelhaes, and S. J. Ram. 2004. Image processing with ImageJ. Biophotonics Int. 11:36-42.
3. Abukhdeir, A. M., and B. H. Park. 2008. p21 and p27: roles in carcinogenesis and drug resistance. Expert Rev. Mol. Med. 10:e19.
4. al-Shawi, R., J. Kinnaird, J. Burke, and J. O. Bishop. 1990. Expression of a foreign gene in a line of transgenic mice is modulated by a chromosomal position effect. Mol. Cell. Biol. 10:1192-1198.
5. Besson, A., S. F. Dowdy, and J. M. Roberts. 2008. CDK inhibitors: cell cycle regulators and beyond. Dev. Cell 14:159-169.
6. Cip1 expression by growth factors and the extracellular matrix reveals a role for transient ERK activity in G1 phase. J. Cell Biol. 146:1255-1264.
7. Bouvard, V., et al. 2000. Tissue and cell-specific expression of the p53-target genes: bax, fas, mdm2 and waf1/p21, before and following ionising irradiation in mice. Oncogene 19:649-660.
8. CDKN1A participates in base excision repair by regulating the activity of poly(ADP-ribose) polymerase-1. DNA Repair (Amst.) 9:627-635.
9. Kip1CDK 'inhibitors' are essential activators of cyclin D-dependent kinases in murine fibroblasts. EMBO J. 18:1571-1583.
10. Dothager, R. S., et al. 2009. Advances in bioluminescence imaging of live animal models. Curr. Opin. Biotechnol. 20:45-53.
11. el-Deiry, W. S. 1998. Regulation of p53 downstream genes. Semin. Cancer Biol. 8:345-357.
12. Ferguson, A. M., L. S. White, P. J. Donovan, and H. Piwnica-Worms. 2005. Normal cell cycle and checkpoint responses in mice and cells lacking Cdc25B and Cdc25C protein phosphatases. Mol. Cell. Biol. 25:2853-2860.
13. Flentie, K. N., et al. 2008. Stably integrated luxCDABE for assessment of Salmonella invasion kinetics. Mol. Imaging 7:222-233.
14. WAFI/Cip1 gene. Exp. Cell. Res. 246:280-289.
15. Gross, S., U. Abraham, J. L. Prior, E. D. Herzog, and D. Piwnica-Worms. 2007. Continuous delivery of D-luciferin by implanted micro-osmotic pumps enables true real-time bioluminescence imaging of luciferase activity in vivo. Mol. Imaging 6:121-130.
16. Gross, S., and D. Piwnica-Worms. 2005. Real-time imaging of ligand-induced IKK activation in intact cells and in living mice. Nat. Methods 2:607-614.
17. Gross, S., and D. Piwnica-Worms. 2005. Spying on cancer: molecular imaging in vivo with genetically encoded reporters. Cancer Cell 7:5-15.
18. Humphries, M. J., et al. 2006. Suppression of apoptosis in the protein kinase C null mouse in vivo. J. Biol. Chem. 281:9728-9737.
19. Klaunig, J. E., et al. 1981. Mouse liver cell culture. I. Hepatocyte isolation. In Vitro 17:913-925.
20. Klaunig, J. E., P. J. Goldblatt, D. E. Hinton, M. M. Lipsky, and B. F. Trump. 1981. Mouse liver cell culture. II. Primary culture. In Vitro 17:926-934.
21. Klerk, C. P., et al. 2007. Validity of bioluminescence measurements for noninvasive in vivo imaging of tumor load in small animals. Biotechniques 43(1 Suppl.):7-13.
22. LaBaer, J., et al. 1997. New functional activities for the p21 family of CDK inhibitors. Genes Dev. 11:847-862.
23. Lee, S., and D. M. Helfman. 2004. Cytoplasmic p21Cip1 is involved in Rasinduced inhibition of the ROCK/LIMK/cofilin pathway. J. Biol. Chem. 279: 1885-1891.
24. Lee, W. J., et al. 2006. Organ-specific gene expressions in C57BL/6 mice after exposure to low-dose radiation. Radiat. Res. 165:562-569.
25. WAF1/CIP1 expression through mitogen-activated protein kinase signaling pathway. Cancer Res. 56:31-35.
26. CIP1. Nature 375:159-161.
27. Macleod, K. F., et al. 1995. p53-dependent and independent expression of p21 during cell growth, differentiation, and DNA damage. Genes Dev. 9:935-944.
28. National Research Council. 1996. Guide for the care and use of laboratory animals. National Academy Press, Washington, DC.
29. Waf1/Cip1cyclindependent kinase inhibitor expression in living animals. Proc. Natl. Acad. Sci. U. S. A. 104:15034-15039.
30. /Cip1 in muscle and other terminally differentiating cells. Science 267:1024-1027.
31. Sarbassov, D. D., D. A. Guertin, S. M. Ali, and D. M. Sabatini. 2005. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307:1098-1101.
32. Sato, T., et al. 2009. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262-265.
33. Sherr, C. J., and R. A. DePinho. 2000. Cellular senescence: mitotic clock or culture shock? Cell 102:407-410.
34. 1-phase progression. Genes Dev. 13:1501-1512.
35. 1 cyclindependent kinases. Genes Dev. 9:1149-1163.
36. Stewart, S. A., et al. 2003. Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 9:493-501.
37. Vasey, D. B., C. R. Wolf, T. MacArtney, K. Brown, and C. B. Whitelaw. 2008. p21-LacZ reporter mice reflect p53-dependent toxic insult. Toxicol. Appl. Pharmacol. 227:440-450.
38. WAF-1/CIP1 expression in the murine intestinal epithelium: apoptosis and cell cycle arrest. Am. J. Pathol. 153:899-909.