Phenotypic characterization and functional analysis of human tumor immune infiltration after mechanical and enzymatic disaggregation

Journal of Immunological Methods

Volumn 372, Issue 1-2, 2011, Pages 119-126

  Grange, Cécile ^a   Létourneau, Jason ^a   Forget, Marie Andrée ^a   Godin Ethier, Jessica ^a   Martin, Jocelyne ^b   Liberman, Moishe ^b   Latour, Mathieu ^b   Widmer, Hugues ^b   Lattouf, Jean Baptiste ^a,b   Piccirillo, Ciriaco A ^c   Cailhier, Jean François ^a   Lapointe, Réjean ^a  

^a NOTRE DAME HOSPITAL   (Canada)

^b CENTRE HOSPITALIER DE L UNIVERSITÉ DE MONTRÉAL   (Canada)

^c RESEARCH INSTITUTE OF THE MCGILL UNIVERSITY HEALTH CENTRE   (Canada)

Author keywords

Collagenase; Homogenization; Medimachine ; Tumor infiltrating immune cells

Indexed keywords

CD14 ANTIGEN; CD4 ANTIGEN; CD45RA ANTIGEN; CD8 ANTIGEN; CELL SURFACE MARKER; COLLAGENASE; DEOXYRIBONUCLEASE; GELATINASE A;

ARTICLE; BREAST TUMOR; CANCER INVASION; CELL ISOLATION; CELL VIABILITY; CONTROLLED STUDY; CYTOLOGY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; KIDNEY TUMOR; LUNG TUMOR; LYMPHOCYTE PROLIFERATION; PHENOTYPE; PRIORITY JOURNAL; SOLID TUMOR; TISSUE DISAGGREGATION; TISSUE INJURY; TUMOR ASSOCIATED LEUKOCYTE;

BREAST NEOPLASMS; CELL PROLIFERATION; CELL SEPARATION; FEMALE; FLOW CYTOMETRY; HUMANS; KIDNEY NEOPLASMS; LUNG NEOPLASMS; LYMPHOCYTES, TUMOR-INFILTRATING;

EID: 80052490349     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2011.07.002     Document Type: Article

References (27)

1. Abuzakouk M., Feighery C., O'Farrelly C. Collagenase and dispase enzymes disrupt lymphocyte surface molecules. J. Immunol. Methods 1996, 194:211.
2. Arlettaz L., Barbey C., Dumont-Girard F., Helg C., Chapuis B., Roux E., Roosnek E. CD45 isoform phenotypes of human T cells: CD4(+)CD45RA(-)RO(+) memory T cells re-acquire CD45RA without losing CD45RO. Eur. J. Immunol. 1999, 29:3987.
3. Cerra R., Zarbo R.J., Crissman J.D. Dissociation of cells from solid tumors. Methods Cell. Biol. 1990, 33:1.
4. Cornacchiari A., Grigolato P.G., Facchetti F., Morassi M.L., Cadei M., Alpi F., Battocchio S., Chirico E. Usefulness of the scraping method for DNA flow cytometry in breast tumors. Cytometry 1995, 19:263.
5. Cunningham R.E. Tissue disaggregation. Methods Mol. Biol. 2010, 588:327.
6. de Visser K.E., Eichten A., Coussens L.M. Paradoxical roles of the immune system during cancer development. Nat. Rev. Cancer 2006, 6:24.
7. Engelholm S.A., Spang-Thomsen M., Brunner N., Nohr I., Vindelov L.L. Disaggregation of human solid tumours by combined mechanical and enzymatic methods. Br. J. Cancer 1985, 51:93.
8. Flynn L., Carton J., Byrne B., Kelehan P., O'Herlihy C., O'Farrelly C. Optimisation of a technique for isolating lymphocyte subsets from human endometrium. Immunol. Invest. 1999, 28:235.
9. Galon J., Costes A., Sanchez-Cabo F., Kirilovsky A., Mlecnik B., Lagorce-Pages C., Tosolini M., Camus M., Berger A., Wind P., Zinzindohoue F., Bruneval P., Cugnenc P.H., Trajanoski Z., Fridman W.H., Pages F. Type, density, and location of immune cells within human colorectal tumors predict clinical outcome. Science 2006, 313:1960.
10. Godin-Ethier J., Pelletier S., Hanafi L.A., Gannon P.O., Forget M.A., Routy J.P., Boulassel M.R., Krzemien U., Tanguay S., Lattouf J.B., Arbour N., Lapointe R. Human activated T lymphocytes modulate IDO expression in tumors through Th1/Th2 balance. J. Immunol. 2009, 183:7752.
11. Grivennikov S.I., Greten F.R., Karin M. Immunity, inflammation, and cancer. Cell 2010, 140:883.
12. Hamburger A.W., Salmon S.E. Primary bioassay of human tumor stem cells. Science 1977, 197:461.
13. Moloney M., Shreffler W.G. Basic science for the practicing physician: flow cytometry and cell sorting. Ann. Allergy Asthma Immunol. 2008, 101:544.
14. Mulder W.M., Koenen H., van de Muysenberg A.J., Bloemena E., Wagstaff J., Scheper R.J. Reduced expression of distinct T-cell CD molecules by collagenase/DNase treatment. Cancer Immunol. Immunother. 1994, 38:253.
15. Novelli M., Savoia P., Cambieri I., Ponti R., Comessatti A., Lisa F., Bernengo M.G. Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T-cell lymphomas. Clin. Exp. Dermatol. 2000, 25:423.
16. Ottesen G.L., Christensen I.J., Larsen J.K., Hansen B., Andersen J.A. Tissue disaggregation for flow cytometric DNA analysis: comparison of fine-needle aspiration and an automated mechanical procedure. Cytometry 1996, 26:65.
17. Pages F., Galon J., Dieu-Nosjean M.C., Tartour E., Sautes-Fridman C., Fridman W.H. Immune infiltration in human tumors: a prognostic factor that should not be ignored. Oncogene 2010, 29:1093.
18. Rasey J.S., Nelson N.J. Effect of tumor dissaggregation on results of in vitro cell survival assay after in vivo treatment of the EMT-6 tumor: X-rays, cyclophosphamide, and bleomycin. In Vitro 1980, 16:547.
19. Salmon S.E., Hamburger A.W., Soehnlen B., Durie B.G., Alberts D.S., Moon T.E. Quantitation of differential sensitivity of human-tumor stem cells to anticancer drugs. N. Engl. J. Med. 1978, 298:1321.
20. Singh N.P. A rapid method for the preparation of single-cell suspensions from solid tissues. Cytometry 1998, 31:229.
21. Stoeck M., Miescher S., Qiao L., Capasso P., Barras C., von Fliedner V. Stimulation of FACS-analysed CD4+ and CD8+ human tumour-infiltrating lymphocytes with ionomycin+phorbol-12,13-dibutyrate does not overcome their proliferative deficit. Clin. Exp. Immunol. 1990, 79:105.
22. Sutherland D.R., Abdullah K.M., Cyopick P., Mellors A. Cleavage of the cell-surface O-sialoglycoproteins CD34, CD43, CD44, and CD45 by a novel glycoprotease from Pasteurella haemolytica. J. Immunol. 1992, 148:1458.
23. Talmadge J.E., Donkor M., Scholar E. Inflammatory cell infiltration of tumors: Jekyll or Hyde. Cancer Metastasis Rev. 2007, 26:373.
24. Van Damme N., Baeten D., De Vos M., Demetter P., Elewaut D., Mielants H., Verbruggen G., Cuvelier C., Veys E.M., De Keyser F. Chemical agents and enzymes used for the extraction of gut lymphocytes influence flow cytometric detection of T cell surface markers. J. Immunol. Methods 2000, 236:27.
25. Van Landuyt K.B., Jones E.A., McGonagle D., Luyten F.P., Lories R.J. Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis. Arthritis Res. Ther. 2010, 12:R15.
26. Vos J.A., Simurdak J.H., Davis B.J., Myers J.B., Brissette M.D. Vortex disaggregation for flow cytometry allows direct histologic correlation: a novel approach for small biopsies and inaspirable bone marrows. Cytometry B Clin. Cytom. 2003, 52:20.
27. Yannelli J.R., Hyatt C., McConnell S., Hines K., Jacknin L., Parker L., Sanders M., Rosenberg S.A. Growth of tumor-infiltrating lymphocytes from human solid cancers: summary of a 5-year experience. Int. J. Cancer 1996, 65:413.