Basic science for the practicing physician: Flow cytometry and cell sorting

Annals of Allergy, Asthma and Immunology

Volumn 101, Issue 5, 2008, Pages 544-549

  Moloney, Marla ^a   Shreffler, Wayne G ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; CYTOKINE; FLUORESCEIN ISOTHIOCYANATE; FLUOROCHROME;

ALLERGIC DISEASE; BASOPHIL; CELL ACTIVATION; CELL COUNT; CELL PROLIFERATION; CELL SELECTION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DIAGNOSTIC VALUE; EOSINOPHILIA; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MEMBRANE POTENTIAL; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REVIEW; SIGNAL TRANSDUCTION; STAINING;

EID: 55849107317     PISSN: 10811206     EISSN: 15344436     Source Type: Journal    
DOI: 10.1016/S1081-1206(10)60295-5     Document Type: Article

References (15)

1. Shapiro HM. Practical Flow Cytometry. 4th ed. Hoboken, NJ: John Wiley & Sons Inc; 2003.
2. De Rosa SC, Herzenberg LA, Roederer M. 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med. 2001;7:245-248.
3. Knol EF, Mul FP, Jansen H, Calafat J, Roos D. Monitoring human basophil activation via CD63 monoclonal antibody 435. J Allergy Clin Immunol. 1991;88:328-338.
4. Lopez-Cabrera M, Santis AG, Fernandez-Ruiz E, et al. Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors. J Exp Med. 1993;178:537-547.
5. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J Immunol Methods. 1994;171:131-137.
6. Wallace PK, Palmer LD, Perry-Lalley D, et al. Mechanisms of adoptive immunotherapy: improved methods for in vivo tracking of tumor-infiltrating lymphocytes and lymphokine-activated killer cells. Cancer Res. 1993;53:2358-2367.
7. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995;188:117-128.
8. Brosterhus H, Brings S, Leyendeckers H, et al. Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur J Immunol. 1999;29:4053-4059.
9. Akdis M, Verhagen J, Taylor A, et al. Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J Exp Med. 2004;199:1567-1575.
10. Brunkow ME, Jeffery EW, Hjerrild KA, et al. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat Genet. 2001;27:68-73.
11. Perez OD, Nolan GP. Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry. Nat Biotechnol. 2002;20:155-162.
12. Ebo DG, Dombrecht EJ, Bridts CH, et al. Combine d analysis of intracellular signalling and immunophenotype of human peripheral blood basophils by flow cytometry: a proof of concept. Clin Exp Allergy. 2007;37:1668-1675.
13. Shapiro HM, Natale PJ, Kamentsky LA. Estimation of membrane potentials of individual lymphocytes by flow cytometry. Proc Natl Acad Sci U S A. 1979;76:5728-5730.
14. Earley MC, Vogt RF Jr, Shapiro HM, et al. Report from a workshop on multianalyte microsphere assays. Cytometry. 2002;50:239-242.
15. Harnett MM. Laser scanning cytometry: understanding the immune system in situ. Nat Rev Immunol. 2007;7:897-904.