Highly expressed cholera toxin B subunit in the fruit of a transgenic tomato (Lycopersicon esculentum L.)

Biotechnology and Bioprocess Engineering

Volumn 16, Issue 3, 2011, Pages 576-580

  Loc, Nguyen Hoang ^a   Thinh, Le Thi ^a   Yang, Moon Sik ^b,c   Kim, Tae Geum ^b  

^a HUE UNIVERSITY   (Viet Nam)

^b Chonbuk National University   (South Korea)

^c Korea Basic Science Institute (KBSI)   (South Korea)

Author keywords

Cholera toxin B subunit; Codon optimization; Edible vaccine; Lycopersicon esculentum

Indexed keywords

A PLANTS; AGROBACTERIUM MEDIATED TRANSFORMATIONS; BIOLOGICAL PROPERTIES; CAMV 35S PROMOTER; CHOLERA TOXIN B SUBUNIT; CODON OPTIMIZATION; CODON USAGE; EDIBLE VACCINES; ENDOPLASMIC RETICULUM; EXPRESSION LEVELS; GENOMIC DNA; IMMUNE RESPONSE; IMMUNOBLOT ANALYSIS; LYCOPERSICON ESCULENTUM; NON-TOXIC MOLECULES; PCR AMPLIFICATION; PENTAMERS; TRANSGENIC PLANTS; TRANSGENIC TOMATO;

BIOSYNTHESIS; FRUITS; GENE ENCODING; GENE EXPRESSION; OPTIMIZATION; POLYMERASE CHAIN REACTION; PROTEINS; TOXIC MATERIALS;

PLANTS (BOTANY);

BACTERIAL PROTEIN; CHOLERA TOXIN B SUBUNIT; GANGLIOSIDE GM1; PLASMID VECTOR; VIRUS PROTEIN;

ARTICLE; BACTERIUM TRANSFORMATION; CODON; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; FRUIT; GENE EXPRESSION; NONHUMAN; PLANT GENOME; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN SYNTHESIS; SIGNAL TRANSDUCTION; TOMATO; TOXIN STRUCTURE; TOXIN SYNTHESIS;

LYCOPERSICON ESCULENTUM;

EID: 80052473343     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-010-0195-8     Document Type: Article

References (23)

1. Kaper, J. B., J. G. Morris, and M. Levine (1995) Cholera. Clin. Microbiol. Rev. 8: 48-86.
2. Field, M., M. C. Rao, and E. B. Chang (1989) Intestinal electrolyte transport and diarrheal disease. N. Engl. J. Med. 321: 800-806. (Pubitemid 19237428)
3. Elson, C. O. (1989) Cholera toxin and its subunits as potential oral adjuvants. Curr. Top. Microbiol. Immunol. 146: 29-33.
4. McGhee, J. R., J. Mestecky, M. T. Dertzbaugh, J. H. Eldridge, M. Hirasawa, and H. Kiyono (1992) The mucosal immune system: From fundamental concepts to vaccine development. Vaccine. 10: 75-88.
5. Jani, D., L. S. Meena, Q. M. Rizwan-ul-Haq, Y. Singh, A. K. Sharma, and A. K. Tyagi (2002) Expression of cholera toxin B subunit in transgenic tomato plants. Transgenic Res. 11: 47-54.
6. Richter, L. J., Y. Thanavala, C. J. Arntzen, and H. S. Mason (2000) Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nat. Biotechnol. 18: 1167-1171.
7. Mason, H. S., T. A. Haq, J. D. Clements, and C. J. Arntzen (1998) Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): Potatoes expressing a synthetic LTB gene. Vaccine 16: 1336-1343. (Pubitemid 28312443)
8. Tuboly, T., W. Yu, A. Bailey, S. Degrandis, S. Du, L. Erickson, and E. Nagy (2000) Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants. Vaccine 18: 2023-2028. (Pubitemid 30114346)
9. Streatfield, S. J., J. M. Jilka, E. E. Hood, D. D. Turner, M. P. Bailey, J. M.Mayor, S. L. Woodard, K. K. Beifuss, M. E. Horn, D. E. Delaney, I. R. Tizard, and J. A. Howard (2001) Plant-based vaccines: Unique advantages. Vaccine 19: 2742-2748. (Pubitemid 32234298)
10. Pelham, H. R. (1988) Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment. EMBO J. 7: 913-918.
11. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473-497.
12. Kang, T. J., N. H. Loc, M. O. Jang, and M. S. Yang (2004) Modification of the cholera toxin B subunit coding sequence to enhance expression in plants. Mol. Breeding 13: 143-153.
13. Van Haute, E., H. Joos, M. Maes, G. Warren, M. Van Montagu, and J. Schell (1983) Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: A novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J. 2: 411-417.
14. Kang, T. J. and M. W. Fawley (1997) Variable (CA/GT)n simple sequence repeat DNA in the alga Chlamydomonas. Plant Mol. Biol. 35: 943-948. (Pubitemid 28016951)
15. Bradford, M. M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72: 248-254.
16. Huy, N. X., Y. S. Kim, S. C. Jun, Z. Jin, S. M. Park, M. S. Yang, and T. G. Kim (2009) Production of a heat-labile enterotoxin B subunit-porcine epidemic diarrhea virus-neutralizing epiyope fusion protein intransgenic lettuce (Lactuca sativa). Biotechnol. Bioproc. Eng. 14: 731-737.
17. Jiang, X. J., Z. M. He, Z. Q. Peng, Y. Qi, Q. Chen, and S. Y. Yu (2007) Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice. Transgenic Res. 16: 169-175. (Pubitemid 46440609)
18. Arakawa, T., D. K. Chong, J. L. Merritt, and W. H. Langridge (1997) Expression of cholera toxin B subunit oligomers in transgenic potato plants. Transgenic Res. 6: 403-413. (Pubitemid 27526605)
19. Matoba, N., H. Kajiura, I. Cherni, J. D. Doran, M. Bomsel, K. Fujiyama, and T. S. Mor (2009) Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR. Plant Biotechnol. J. 7: 129-145.
20. Kim, Y. S., B. G. Kim, T. G. Kim, T. J. Kang, and M. S. Yang (2006) Expression of a cholera toxin B subunit in transgenic lettuce (Lactuca sativa L.) using Agrobacterium-mediated transformation system. Plant Cell Tiss. Org. Cult. 87: 203-210.
21. Oszvald, M., T. J. Kang, S. Tomoskozi, B. Jenes, T. G. Kim, Y. S. Cha, L. Tamas, and M. S. Yang (2008) Expression of cholera toxin B subunit in transgenic rice endosperm. Mol. Biotechnol. 40: 261-268.
22. Kang, T. J., B. G. Kim, J. Y. Yang, and M. S. Yang (2007) Expression of a synthetic cholera toxin B subunit in tobacco using ubiquitin promoter and bar gene as a selectable marker. Mol. Biotechnol. 32: 93-100.
23. Lim, J. G. and H. S. Jin (2008) Heterologous expression of cholera toxin B subunit in Saccharomyces cerevisiae. Biotechnol. Bioproc. Eng. 13: 598-605.